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ID   PUC13CAT   preliminary; circular DNA; SYN; 4255 BP.
XX
AC   IG9863;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pUC13CAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plambdaTF15 from lambda & Xenopus TFIIIA promoter
RC   pUC13CAT from pUC13 & cat gene
RC   pCATF9, pCATF15, pCATF16, pCATF30 from pUC13CAT & plambdaTF15
RC   pCATF35 from pCATF9 & pCATF16
RC   pCATF34 from pCATF9 & pCATF15
RC   pCATF36 from pCATF9 & pCATF30
RA   Hall R.K., Taylor W.L.;
RT   "Transcription factor IIIA gene expression in Xenopus oocytes
RT   utilizes a transcription factor similar to the major late
RT   transcription factor";
RL   Mol. Cell. Biol. 9:5003-5011(1989).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUC13CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC13)(pSV2-cat)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC13 SalI 2680bp 249..249
FT                   fill in:fill in
FT                   2. pSV2-cat RsaI-RsaI 1571bp 2106..3677,
FT                   \ SV40 promoter/cat gene
FT                   -> pUC13CAT 4251bp"
FT   -               1..252
FT                   /note="pUC13 1..252 252bp
FT                   SalI = G^TCGA C
FT                   RsaI =     GT^AC"
FT   -               253..1823
FT                   /note="pSV2-cat 2106..3676 1571bp
FT                   RsaI = GT^AC
FT                   SalI =  G^TCGAC"
FT   -               1824..4255
FT                   /note="pUC13 249..2680 2432bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4255 BP; 1141 A; 987 C; 1020 G; 1107 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     gctgcaggtc gaactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc ctttgcctaa
     tttaaatgag gacttaacct gtggaaatat tttgatgtgg gaagctgtta ctgttaaaac
     tgaggttatt ggggtaactg ctatgttaaa cttgcattca gggacacaaa aaactcatga
     aaatggtgct ggaaaaccca ttcaagggtc aaattttcat ttttttgctg ttggtgggga
     acctttggag ctgcagggtg tgttagcaaa ctacaggacc aaatatcctg ctcaaactgt
     aaccccaaaa aatgctacag ttgacagtca gcagatgaac actgaccaca aggctgtttt
     ggataaggat aatgcttatc cagtggagtg ctgggttcct gatccaagta aaaatgaaaa
     cactagatat tttggaacct acacaggtgg ggaaaatgtg cctcctgttt tgcacattac
     taacacagca accacagtgc ttcttgatga gcagggtgtt gggcccttgt gcaaagctga
     cagcttgtat gtttctgctg ttgacatttg tgggctgttt accaacactt ctggaacaca
     gcagtggaag ggacttccca gatattttaa aattaccctt agaaagcggt ctgtgaaaaa
     cccctaccca atttcctttt tgttaagtga cctaattaac aggaggacac agagggtgga
     tgggcagcct atgattggaa tgtcctctca agtagaggag gttagggttt atgaggacac
     agaggagctt cctggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt attaacccct
     ttacaaatta aaaagctaaa ggttcgactc tagaggatcc ccgggcgagc tcgaattcac
     tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//