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ID   PUC18      preliminary; circular DNA; SYN; 2686 BP.
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AC   L08752; A02710; VB0025; ATCC37253;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUC18 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
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RN   [2]
RP   1-2686
RC   pUC18
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   Expression Vector For Adjustable Expression Of Exogenous
CC   Genes In Prokaryotes; Patent number WO8809373-A/8 (01-DEC-1988).
CC   This patent sequence is not up-to-date.
CC
CC   Expression vector with lacZ' as an insertional detection marker.
CC   Medium is 1227 LB plus ampicillin.
CC   GenBank entry #L08752 is not current with commercial entries.
CC   NM (pUC18)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)(BRL)(CLONTECH)(IBI)(Pharmacia)(Sigma)
CC   SP (USB)(Worthington)(ATCC)
CC   HO (E.coli JM103)(E.coli DH5alpha)(E.coli DH10B)(E.coli TB1)
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)(E.coli NM522)
CC   HO (E.coli K-12)(E.coli)
CC   CP (high)
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pBR322)(M13mp18)(pUC12)
CC   BR (pUC19)
CC   OF (pGEM-3)(pTZ18R)(pIBI24)(pIBI30)(pSP18)(pT7T3-18)(pT7T3alpha-18)
CC   OF (pES1)(pES2 from pES1)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove EcoRI-HindIII 45bp 231..276,
FT                   \ MCS/2635bp
FT                   2. M13mp18 EcoRI-HindIII 51bp 6231..6282, MCS
FT                   -> pUC18 2686bp"
FT   misc_feature    1..2616
FT                   /note="PvuII-EcoRI fragment of pBR322"
FT   misc_feature    1..2616
FT                   /note="HaeII fragment of M13mp18"
FT   misc_feature    1..1
FT                   /note="base 1 is base 2074 of pBR322"
FT   misc_binding    51..51
FT                   /note="SIT unique Esp3I"
FT   misc_binding    91..91
FT                   /note="SIT double DrdI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoO109I"
FT   misc_binding    181..181
FT                   /note="SIT unique HgiEII"
FT   misc_binding    183..183
FT                   /note="SIT unique NdeI"
FT   misc_binding    235..235
FT                   /note="SIT unique NarI"
FT   misc_binding    235..235
FT                   /note="SIT triple HaeII (lac fragment)"
FT   CDS             complement(0..0)
FT                   /note="PRO E. coli lacZ gene"
FT   CDS             complement(236..469)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     364..386
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     379..395
FT                   /note="PRI bacteriophage M13 forward"
FT   misc_binding    399..399
FT                   /note="SIT unique HindIII"
FT   misc_binding    399..450
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   BspMI-PstI-SphI-HindIII"
FT   misc_binding    450..450
FT                   /note="SIT unique EcoRI"
FT   primer_bind     461..476
FT                   /note="PRI bacteriophage T7T3alpha"
FT   CDS             complement(470..591)
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    680..680
FT                   /note="SIT triple HaeII (lac fragment)"
FT   misc_binding    806..806
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(861..861)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   misc_binding    908..908
FT                   /note="SIT double DrdI"
FT   misc_binding    1217..1217
FT                   /note="SIT unique AlwNI"
FT   misc_binding    1387..1387
FT                   /note="SIT unique HgiEI"
FT   CDS             complement(1626..2486)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1779..1779
FT                   /note="SIT unique Cfr101"
FT   misc_binding    1784..1784
FT                   /note="SIT unique GsuI"
FT   misc_binding    2177..2177
FT                   /note="SIT unique ScaI"
FT   misc_binding    2215..2215
FT                   /note="SIT unique BcgI"
FT   misc_binding    2294..2294
FT                   /note="SIT unique XmnI"
FT   misc_binding    2501..2501
FT                   /note="SIT unique SspI"
FT   misc_binding    2531..2531
FT                   /note="SIT unique BsmAI"
FT   misc_binding    2617..2617
FT                   /note="SIT unique AatII"
FT   misc_binding    2674..2674
FT                   /note="SIT unique DraII"
XX
SQ   Sequence 2686 BP; 668 A; 677 C; 683 G; 658 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aaagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//