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ID   PUC1813    preliminary; circular DNA; SYN; 2734 BP.
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AC   X02514; X02513; ATCC37523;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUC1813 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC1813 from pUC18 & pUC13
RC   pUC1318 from pUC18 & pUC13
RA   Kay R., McPherson J.;
RT   "Hybrid pUC vectors for addition of new restriction enzyme sites
RT   to the ends of DNA fragments";
RL   Nucleic Acids Res. 15:2778-2778(1987).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   A pUC type plasmid vector with polylinker designed for addition of new
CC   restriction sites to the ends of DNA fragments.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--2.66; HindIII--2.54; KpnI--2.66. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUC1813)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM105)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)(pUC13)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 XmnI-EcoRI 838bp 2299..2686..451,
FT                   \ [837bp]
FT                   2. pUC13 XmnI-EcoRI 1892bp 1067..2680..279, [1890bp]
FT                   -> pUC1813 2730bp"
FT   -               1..1892
FT                   /note="pUC13 1067..2680..278 1892bp
FT                   EcoRI = G^AATTC"
FT   -               1893..2734
FT                   /note="pUC18 2299..2686..454 842bp complement
FT                   XmnI = GAANN^NNTTC"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-HincII-XbaI-BamHI-SmaI-SacI-
FT                   EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-HincII-PstI-SphI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique HincII-XbaI-BamHI-SmaI-SacI-EcoRI-
FT                   KpnI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2734 BP; 667 A; 699 C; 694 G; 674 T; 0 other;
     ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca
     ggtcgactct agaggatccc cgggcgagct cgaattcgag ctcggtaccc ggggatcctc
     tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact
     gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     gtaagatcct tgagagtttt cgccccgaag aacg
//