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ID   PUC18LUC   preliminary; DNA; INV; 5411 BP.
XX
AC   U03687;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Photinus pyralis pUC18-luciferase - complete.
XX
KW   cloning vector.
XX
OS   Photinus pyralis
OC   Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
OC   Pterygota; Neoptera; Holometabola; Coleoptera; Polyphaga;
OC   Cantharoidea; Lampyridae.
XX
RN   [1]
RP   1-5411
RC   pUC18-luciferase from pUC18 & firefly luciferase gene
RA   Bonin A.L., Gossen M., Bujard H.;
RT   "Photinus pyralis luciferase: vectors that contain a modified
RT   coding sequence allowing convenient transfer into other systems";
RL   Unpublished (1995).
XX
RN   [2]
RP   1-5411
RC   pUC18-luciferase from pUC18 & firefly luciferase gene
RA   Bonin A.L.;
RT   ;
RL   Submitted (23-NOV-1993) to the EMBL/GenBank/DDBJ database by:
RL   Bonin A.L., Zentrum fuer Molekulare Biologie, Universit Heidelberg,
RL   Im Neuenheimer Feld 282, Heidelberg, 69120, GERMANY.
XX
CC   NM (pUC18-luciferase)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP (high)
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 789bp 1..789
FT                   2. Photinus pyralis 1700bp 790..2490, luciferase gene
FT                   3. SV40 768bp 2496..3264
FT                   4. pUC18 2920bp 2491..5411
FT                   -> pUC18-luciferase 5411bp"
FT   promoter        14..760
FT                   /note="PRO human CMV immediate-early region (IE)"
FT   intron          2496..3264
FT                   /note="from SV40 small t"
FT   polyA_signal    3265..3271
FT                   /note="PLA SV40"
FT   CDS             790..2442
FT                   /note="conservative nucleotide exchanges in CDS"
XX
SQ   Sequence 5411 BP; 1492 A; 1225 C; 1227 G; 1467 T; 0 other;
     gaattcctcg aggagcttgg cccattgcat acgttgtatc catatcataa tatgtacatt
     tatattggct catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa
     tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     tatttacgct aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgag
     ctcggtacca tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgcta
     gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
     gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
     aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
     taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaac
     tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
     agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
     caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
     agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
     cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
     caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
     atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
     ttgaagtctt taattaaata caaaggatac caggtggccc ccgctgaatt ggagtcgata
     ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
     ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     atcgtggatt acgtcgccag tcaagtaaca accgccaaaa agttgcgcgg aggagttgtg
     tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac tgtattcagc
     gatgacgaaa ttcttagcta ttgtaatact ctagaggatc tttgtgaagg aaccttactt
     ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     taaaggggtt aataaggaat atttgatgta tagtgccttg atcataatca gccataccac
     atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     tttgtccaaa ctcatcaatg tatcttatca tgtctgcctc tagagctgca ttaatgaatc
     ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtcttca a
//