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ID   PUC4       preliminary; circular DNA; SYN; 2605 BP.
XX
AC   IG0112;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUC4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC81 from pUC7
RC   pUC91 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
XX
CC   old Pharmacia vector.
CC   NM (pUC4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM83)(E.coli JM103)
CC   CP ()
CC   FN (cloning)(expression)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUR1)
CC   BR (pUC3)(pUC5)(pUC7)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC3 HaeII 2223bp 216..216, may be 586..586
FT                   2. M13mp7 HaeII-HaeII 434bp 5572..6006, lac
FT                   -> pUC4 2605bp
FT                   1. pUR1 2223bp
FT                   2. M13mp2, MCS
FT                   -> pUR2 2300bp
FT                   1. pUR2 PstI 2300bp 1476..1476, amp gene
FT                   mutagenesis
FT                   EcoRI 2300bp, M13mp2 MCS
FT                   2. M13mp7 EcoRI-HincII/HindII 17bp 6232..6249, MCS
FT                   -> pUC4 2605bp"
FT   CDS             complement(236..457)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   promoter        complement(458..579)
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      849..849
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   CDS             complement(1614..2475)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2605 BP; 649 A; 654 C; 662 G; 640 T; 0 other;
     gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     acgacgttgt aaaacgacgg ccagtgaatt ccccggatcc gtcgacctgc aggtcgacgg
     atccggggaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     tagactaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     ctacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtc
//