back Return to this vector's summary.
ID   PUC4CM     preliminary; circular DNA; SYN; 4240 BP.
XX
AC   IG9850;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pUC4CM - complete, MCS.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC4CM from pUC4K & pBR325, E. coli cat gene
RC   M13mp18CML, M13mp18CMR from M13mp18 & pUC4CM, E. coli cat gene
RC   pUC18CML from pUC18 & M13mp18CML, E. coli cat gene
RC   pUC18CMR from pUC18 & M13mp18CMR, E. coli cat gene
RA   Schweizer H.P.;
RT   "The pUC18CM plasmids: a chloramphenicol resistance gene cassette
RT   for site-directed insertion and deletion mutagenesis in
RT   Escherichia coli";
RL   Biotechniques 8:612-616(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUC4CM)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli XL-1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR325)(pUC4K)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC4K HincII-HincII 2662bp 1666..3914..414
FT                   2. pBR325 HincII-BclI 1574bp 3910..5484, E. coli cat
FT                   :Klenow
FT                   -> pUC4CM 4236bp [no HincII sites]"
FT   -               1..2662
FT                   /note="pUC4K 1666..3914..413 2662bp
FT                   HindII = HincII = GTY^RAC"
FT   -               2663..4240
FT                   /note="pBR325 3910..5487 1578bp complement
FT                   BclI =         T^GATC A
FT                   HindII = HincII = GTY^RAC"
FT   misc_binding    0..0
FT                   /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SmaI-KpnI-SstI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4240 BP; 1099 A; 1026 C; 1015 G; 1100 T; 0 other;
     gacggatccg gggaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     cagtgaattc cccggatccg tcgatcatat cgtcaattat tacctccacg gggagagcct
     gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc
     gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
     cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     ggtatttatt cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
//