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ID   PUC4K      preliminary; circular DNA; SYN; 3914 BP.
XX
AC   X06404; IG0113;
XX
DT   02-APR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUC4K - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3914
RC   pUC4K
RA   Taylor L.A.;
RT   ;
RL   Submitted (15-DEC-1987) on tape to the EMBL Data Library by:
RL   Taylor L.A., Calgene Inc., 1920 Fifth St., Davis, CA 05616, USA.
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RN   [2]
RP   389-1690
RC   pUC4K from pUC4 & Tn903, kan gene
RA   Taylor L.A., Rose R.E.;
RT   "A Correction in the Nucleotide Sequence of the Tn903 Kanamycin
RT   Resistance Determinant in pUC4K";
RL   Nucleic Acids Res. 16:358-358(1988).
XX
RN   [3]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC81 from pUC7
RC   pUC91 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
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RN   [4]
RC   Tn903 or Tn601 from E. coli, kan gene [in R6K]
RC   Tn601 or Tn55 from E. coli, kan gene [in R6K]
RC   pAO2 from ColE1
RC   pAO43 from Tn903 & pAO2
RA   Oka A., Sugisaki H., Takanami M.;
RT   "Nucleotide sequence of the kanamycin resistance transposon Tn903";
RL   J. Mol. Biol. 147:217-226(1981).
XX
RN   [5]
RC   pAO3 from ColE1
RC   pAO43 from pAO2 & R6-5, Tn903 kan gene
RC   pAO48 from pAO2 & R6-5, Tn903 kan gene
RC   pAO65 from pAO3 & R6-5, Tn903 kan gene
RA   Oka A., Nomura N., Sugimoto K., Sugisaki H., Takanami M.;
RT   "Nucleotide sequence at the insertion sites of a kanamycin
RT   transposon";
RL   Nature 276:845-847(1978).
XX
RN   [6]
RC   pAO2 from ColE1
RA   Oka A.;
RT   "Fine cleavage map of a small colicin E1 plasmid carrying genes
RT   responsible for replication and colicin E1 immunity";
RL   J. Bacteriol. 133:916-924(1978).
XX
RN   [7]
RC   pUC4K from pUC4 & Tn903, kan gene
RC   pUC4-KISS from pUC4 & Tn903, kan gene
RA   Barany F.;
RT   "New plasmids for selection of TAB linker mutations";
RL   Analects [Pharmacia] 13/14:5-6(1985).
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CC   NM (pUC4K)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (vector construction)(codon insertions)
CC   SE (color blue/white)
CC   PA (pUC7)(Tn903)(pUC4)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pUC7 PstI 2674bp 256..256
FT                   dCG tailing
FT                   2. pML21 HaeII-HaeII 1398bp 1371..2769,
FT                   \ Tn903 kan/aph I gene
FT                   \ pVH51 HaeII 1012 1371 2688 2769
FT                   dCG tailing
FT                   -> pUC4K 3914bp"
FT   CDS             complement(236..1697)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    396..396
FT                   /note="SIT double EcoRI"
FT   misc_binding    405..405
FT                   /note="SIT double BamHI"
FT   misc_binding    411..411
FT                   /note="SIT double SalI"
FT   misc_binding    417..417
FT                   /note="SIT double PstI"
FT   misc_feature    complement(433..1645)
FT                   /note="HaeII-HaeII fragment of Tn903 (835..2265)
FT                   inserted into PstI site of pUC4"
FT   CDS             complement(720..1535)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan),
FT                   aminoglycoside 3'-phosphotransferase gene (APH),
FT                   from Tn903 PstI-PstI fragment"
FT   misc_binding    1657..1657
FT                   /note="SIT double PstI"
FT   misc_binding    1663..1663
FT                   /note="SIT double SalI"
FT   misc_binding    1669..1669
FT                   /note="SIT double BamHI"
FT   misc_binding    1678..1678
FT                   /note="SIT double EcoRI"
FT   promoter        complement(1698..1819)
FT                   /note="PRO E. coli lac operator"
FT   CDS             complement(2854..3714)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3914 BP; 1011 A; 968 C; 949 G; 986 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccccggatcc gtcgacctgc
     aggggggggg gggcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
     ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt
     gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg
     ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat
     tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     agattttgag acacaacgtg gctttccccc ccccccctgc aggtcgacgg atccggggaa
     ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtc
//