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ID   PUC7       preliminary; circular DNA; SYN; 2674 BP.
XX
AC   L08958; VB0020; M10291;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUC7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC81 from pUC7
RC   pUC91 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
XX
RN   [2]
RP   1-2674
RC   pUC7
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC19 and M13mp7 by F. Pfeiffer, MPI, Martinsried
CC   NM (pUC7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM103)
CC   CP ()
CC   FN (cloning)(expression)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (M13mp7)(pBR322)
CC   BR (pUC3)(pUC4)(pUC5)
CC   OF (pUC8)(pUC9)(pUR222)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC5 AccI 2223bp 110..110
FT                   BAL31 nuclease
FT                   -> pUC6 2220bp [no AccI]
FT                   1. pUC6 HaeII 2220bp 216..216, may be 586..586
FT                   2. M13mp7 HaeII-HaeII 433bp 6006..6439, lac
FT                   -> pUC7 2674bp"
FT   CDS             1..229
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    230..277
FT                   /note="MCS EcoRI-BamHI-SalI-PstI-SalI-BamHI-EcoRI
FT                   polylinker of M13mp7"
FT   CDS             78..435
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   CDS             940..1728
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2674 BP; 656 A; 682 C; 673 G; 663 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattccccgg
     atccgtcgac ctgcaggtcg acggatccgg ggaattcact ggccgtcgtt ttacaacgtc
     gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     agcgcagcga gtcagtgagc gaggaagcgg aaga
//