back Return to this vector's summary.
ID   PUC830     preliminary; circular DNA; SYN; 2710 BP.
XX
AC   L09142; VB0056; ATCC77218;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUC830 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC830 from pUC8 & M13tg131
RC   pUC931 from pUC9 & M13tg131
RC   pOM1 from pUC8 & M13tg131
RC   pOM2 from pUC9 & M13tg131
RC   pOM3 from pUC8 & M13tg131
RC   pOM4 from pUC9 & M13tg131
RC   pOM8 from pUC8 & M13tg131
RC   pOM9 from pUC9 & M13tg131
RA   Oberbaumer I.;
RT   "New pUC-derived expression vectors for rapid construction of cDNA
RT   libraries";
RL   Gene 49:81-91(1986).
XX
RN   [2]
RP   1-2710
RC   pUC830
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Expression vector useful for construction of cDNA libraries and
CC   containing priming sites useful for sequencing.  Permits visual
CC   detection of recombinants by beta-galactosidase alpha peptide
CC   complementation. [1]
CC   pUC830 (ATCC 77218) and pUC931 (ATCC 77219) differ in the orientation
CC   of the polylinker. [1]
CC   E.coli transformed with this plasmid produce larger colonies
CC   when an insert is present in the polylinker than when there is no
CC   insert.
CC   (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7; HindIII--2.7; EcoRI--2.7; NheI--2.7. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUC830)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)(M13tg130)(bGal130)
CC   BR (pUC931)
CC   OF (pOM2)(pOM4)(pOM8)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HindIII 2665bp 261..261
FT                   Klenow
FT                   remove PstI-EcoRI 26bp 231..257, MCS/2639bp
FT                   2. M13tg131 PstI-EcoRI 59bp 6242..6301, MCS
FT                   -> pUC830 2710bp"
FT   misc_binding    230..298
FT                   /note="MCS EcoRI-SmaI-SacI-EcoRV-SphI-KpnI-XbaI-
FT                   HindIII-BamHI-SalI-PstI; M13tg130-Polylinker"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             978..1766
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2710 BP; 666 A; 690 C; 683 G; 671 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     agagctcgat atcgcatgcg gtacctctag aagaagcttg ggatccgtcg acctgcagcc
     aagctagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     aagcggaaga
//