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ID   PUC8CM     preliminary; circular DNA; SYN; 3438 BP.
XX
AC   IG9930;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pUC8CM - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC8CM from pUC8 & cat gene
RC   pKJB1 from pUC8CM
RC   pKJB231.1 from pKJB1
RA   Buckley K.;
RT   ;
RL   Unpublished (1984).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUC8CM)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pUC8)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 2665bp, TaqI = 248 998 2442
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pUC8CM 3438bp"
FT   -               1..247
FT                   /note="pUC8 1..247 247bp
FT                   TaqI = T^CGA"
FT   -               248..1020
FT                   /note="Tn9 215..987 773bp
FT                   TaqI = T^CGA"
FT   -               1021..3438
FT                   /note="pUC8 248..2665 2418bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3438 BP; 865 A; 842 C; 850 G; 881 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     cgacctgcag ccaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     gagcgaggaa gcggaaga
//