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ID   PUC8NOT    preliminary; circular DNA; SYN; 2664 BP.
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AC   M18133; ATCC37572;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUC8-Not - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRK419lac from pRK419 & pAD3
RC   pcos4EMBLlac from pcos4EMBL & pAD3
RC   pcos1EMBL from pcos4EMBLlac & pRK419lac
RC   cos793 from pcos1EMBL
RC   cos798 from cos793
RC   cosU800 from pcos1EMBL & pUC9
RC   cos2801 from cos798 & cosU800
RC   pcos2EMBL from cos2801
RC   cos786 from pcos1EMBL & pGD3
RC   cos790 from cos786
RC   cos806 from cos790
RC   cos807 from cos806
RC   cos808 from cos807
RC   cos809 from cos808 & pcos2EMBL
RC   cos810K from cos809
RC   pcos3EMBL from cos810K
RC   cos851 from pcos3EMBL & pUC2
RC   cos852 from cos851
RC   pUC7deltaSal from pUC7
RC   cos854 from cos852 & pUC7deltaSal
RC   pcos5EMBL from cos854
RC   pM1380 from pir41 gene
RC   cos860 from pcos5EMBL & pM1380, pir41 gene
RC   pcos6EMBL from cos860
RC   pEMBL18-Not from R6K
RA   Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT   "A family of cosmid vectors with the multi-copy R6K replication
RT   origin";
RL   Gene 57:229-237(1987).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Polylinker of pUC8 modified.
CC   General purpose vector modified to include a NotI site to facilitate
CC   library construction and chromosome walking.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7; BamHI--uncut; EcoRI--2.7. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUC8-Not)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 remove EcoRI-SalI 16bp 231..247,
FT                   \ MCS/2649bp
FT                   :fill in
FT                   2. NotI linker 15bp aattcccgcggccgc
FT                   -> pUC8-Not 2664bp"
FT   -               1..230
FT                   /note="pUC8 1..230 230bp
FT                   EcoRI = G^AATTC
FT                   \         aattcccgcggccgc"
FT   -               231..245
FT                   /note="aattcccgcggccgc 15bp
FT                   \ aattcccgcggccgc
FT                   SalI =          G^TCGAC"
FT   -               246..2664
FT                   /note="pUC8 247..2665 2419bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-NotI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-NotI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
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SQ   Sequence 2664 BP; 653 A; 682 C; 669 G; 660 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccgcg
     gccgctcgac ctgcagccaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     gtcagtgagc gaggaagcgg aaga
//