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ID   PUC9       preliminary; circular DNA; SYN; 2665 BP.
XX
AC   L09128; VB0022; M77827; M77690; M10293; ATCC37252;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUC9 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC81 from pUC7
RC   pUC91 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
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RN   [2]
RC   from pUC8
RA   Helfman D.M., Feramisco J.R., Fiddes J.C., Thomas G.P., Hughes S.H.;
RT   "Identification of clones that encode chicken tropomyosin by direct
RT   immunological screening of a cDNA expression library";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:31-35(1983).
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RN   [3]
RC   from pUC9
RA   Heidecker G., Messing J.;
RT   "Sequence analysis of zein cDNAs obtained by an efficient mRNA
RT   cloning method";
RL   Nucleic Acids Res. 11:4891-4906(1983).
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RN   [4]
RP   1-2695
RC   pUC9
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC19 and M13MP9 by F. Pfeiffer, MPI, Martinsried
CC   old Pharmacia vector.
CC   Expression vector with lacZ as an insertion detection marker.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUC9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (NEB)(Sigma)(ATCC)
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)(E.coli NM522)(E.coli)
CC   CP ()
CC   FN (cloning)(transcription)(mutagenesis)(sequencing)
CC   SE (color blue/white)
CC   PA (pUC7)(M13mp9)
CC   BR (pUC8)
CC   OF (pUC13)(pEMBL9-)(pEMBL9+)(pUC931)(pOM1)(pOM3)(pOM9)(pUC9-1)(pUC9-2)
CC   OF (pIC19H)(pIC19R)(pICEM19H-)(pICEM19H+)(pICEM19R-)(pICEM19R+)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   linker
FT                   -> M13mLL2 7200bp
FT                   1. pUC4K BamHI-BamHI 1270bp 406..1670, kan gene
FT                   2. M13mLL2 BamHI 7200bp 2221..2221
FT                   -> M13mLL2K 8500bp
FT                   1. M13mLL2K EcoRI-EcoRI 1270bp 2221..3491, kan gene
FT                   \ pUC4K 406..1670
FT                   2. pUC7 EcoRI 2674bp 231..231, may be 273..273
FT                   -> pUC71K 3944bp
FT                   1. pUC71K remove EcoRI-EcoRI 42bp 231..273, 3902bp
FT                   S1 nuclease
FT                   HindIII linker 10bp ccaagcttgg:PstI linker 9bp
FT                   \ gctgccagc
FT                   remove PstI-PstI 1200bp, kan gene/redundant sites
FT                   -> pUC9 2665bp [HindIII site added on PstI site"
FT   misc_binding    0..0
FT                   /note="SIT unique Esp3I"
FT   misc_binding    0..0
FT                   /note="SIT double DrdI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoO109I"
FT   misc_binding    0..0
FT                   /note="SIT unique HgiEII"
FT   misc_binding    0..0
FT                   /note="SIT unique NdeI"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        1..230
FT                   /note="GEN E. coli lacZPO promoter"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 forward"
FT   misc_binding    233..268
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   polylinker of M13mp9"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage T7T3alpha"
FT   CDS             269..426
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT double DrdI"
FT   misc_binding    0..0
FT                   /note="SIT unique AlwNI"
FT   misc_binding    0..0
FT                   /note="SIT unique HgiEI"
FT   CDS             931..1719
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique Cfr101"
FT   misc_binding    0..0
FT                   /note="SIT unique GsuI"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BcgI"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SspI"
FT   misc_binding    0..0
FT                   /note="SIT unique BsmAI"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   misc_binding    0..0
FT                   /note="SIT unique DraII"
XX
SQ   Sequence 2665 BP; 654 A; 679 C; 671 G; 661 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatcccc gggaattcac tggccgtcgt tttacaacgt cgtgactggg
     aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     agtcagtgag cgaggaagcg gaaga
//