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ID   PUC91      preliminary; circular DNA; SYN; 2695 BP.
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AC   L09140; VB0047;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUC9-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2695
RC   pUC9-1
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pUC8-1, pUC8-2 from pUC8
RC   pUC9-1, pUC9-2 from pUC9
RA   Hanna Z., Fregeau C., Prefontaine G., Brousseau R.;
RT   "Construction of a family of universal expression plasmid vectors";
RL   Gene 30:247-250(1984).
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RN   [3]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC8-1 from pUC7
RC   pUC9-1 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC9 and pUV91a by F. Pfeiffer, MPI, Martinsried
CC   The pUC vectors contain the beta-galactosidase gene under the
CC   control of the lac-promoter and lac-operator. Thus they are
CC   usable as inducible expression vectors. To allow insertion of
CC   genes in any reading frame, the polylinker region was
CC   redesigned to contain the same restriction sites in all three
CC   reading frames:
CC   Trio '8' is pUC8, pUC8-1 and pUC8-2.
CC   Trio '9' is pUC9, pUC9-1 and pUC9-2.
CC   NM (pUC9-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)(E.coli NM522)
CC   CP ()
CC   FN (cloning)(transcription)(mutagenesis)(sequencing)
CC   SE (color blue/white)
CC   PA (pUC9)(pUC91a)
CC   BR (pUC8-1)(pUC8-2)(pUC9-2)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   linker
FT                   -> M13mLL2 7200bp
FT                   1. pUC4K BamHI-BamHI 1270bp 406..1670, kan gene
FT                   2. M13mLL2 BamHI 7200bp 2221..2221
FT                   -> M13mLL2K 8500bp
FT                   1. M13mLL2K EcoRI-EcoRI 1270bp 2221..3491, kan gene
FT                   \ pUC4K 406..1670
FT                   2. pUC7 EcoRI 2674bp 231..231, may be 273..273
FT                   -> pUC71K 3944bp
FT                   1. pUC71K remove EcoRI-EcoRI 42bp 231..273, 3902bp
FT                   S1 nuclease
FT                   HindIII linker 10bp ccaagcttgg:PstI linker 9bp
FT                   \ gctgccagc
FT                   remove PstI-PstI 1200bp, kan gene/redundant sites
FT                   -> pUC9-1 2695bp [HindIII site added on PstI site"
FT   CDS             1..234
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    230..265
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             961..1749
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2695 BP; 661 A; 687 C; 679 G; 668 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctccc
     aagcttggga gcttggctgc aggtcgacgg atccccggga attgcgaatt cgcaattcac
     tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//