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ID PUC92 preliminary; circular DNA; SYN; 2695 BP.
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AC L09141; VB0048;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pUC9-2 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-2695
RC pUC9-2
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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RN [2]
RC pUC8-1, pUC8-2 from pUC8
RC pUC9-1, pUC9-2 from pUC9
RA Hanna Z., Fregeau C., Prefontaine G., Brousseau R.;
RT "Construction of a family of universal expression plasmid vectors";
RL Gene 30:247-250(1984).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled from pUC9 and pUV91a by F. Pfeiffer, MPI, Martinsried
CC The pUC vectors contain the beta-galactosidase gene under the
CC control of the lac-promoter and lac-operator. Thus they are
CC usable as inducible expression vectors. To allow insertion of
CC genes in any reading frame, the polylinker region was
CC redesigned to contain the same restriction sites in all three
CC reading frames:
CC Trio '8' is pUC8, pUC8-1 and pUC8-2.
CC Trio '9' is pUC9, pUC9-1 and pUC9-2.
CC NM (pUC9-2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli JM83)(E.coli JM105)(E.coli JM109)(E.coli NM522)
CC CP ()
CC FN (cloning)(transcription)(mutagenesis)(sequencing)
CC SE (color blue/white)
CC PA (pUC9)(pUC92b)
CC BR (pUC8-1)(pUC8-2)(pUC9-1)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC9 2665bp
FT -> pUC9-2 2695bp"
FT CDS 1..234
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_binding 230..265
FT /note="MCS unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 961..1749
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 2695 BP; 661 A; 687 C; 679 G; 668 T; 0 other;
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctgca
agcttgcagc ttggctgcag gtcgacggat ccccgggaat tgcggaattc cgcaattcac
tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//