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ID   PUC9TET    preliminary; circular DNA; SYN; 4007 BP.
XX
AC   IG0118;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUC9tet - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC3 from pUR1
RC   pUC4 from M13mp7 & pUC3
RC   pUC4K from pUC4 & pML21, kan gene
RC   [pUC4K from pUC7 & Tn903, kan gene]
RC   M13mLL2K from pUC4K & M13mLL2
RC   pUC5 from pUC3
RC   pUC6 from pUC5
RC   pUC7 from pUC6 & M13mp7
RC   pUC71K from pUC7 & M13mLL2K
RC   pUC8 from pUC7
RC   pUC9 from pUC7
RC   pUC81 from pUC7
RC   pUC91 from pUC7
RA   Vieira J., Messing J.;
RT   "The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
RT   and sequencing with synthetic universal primers";
RL   Gene 19:259-268(1982).
XX
CC   old Pharmacia vector.
CC   NM (pUC9tet)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(promoter analysis)
CC   SE (color blue/white)
CC   PA (pUC9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC9 2665bp
FT                   2. pBR322 1342bp, tet gene
FT                   -> pUC9tet 4007bp"
FT   CDS             467..1657
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(1633..1854)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(1855..1975)
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      2244..2244
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   CDS             complement(3009..3879)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4007 BP; 896 A; 1096 C; 1064 G; 951 T; 0 other;
     gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     acgacgttgt aaaacgacgg ccagggccag tgaattccca agctttaatg cggtagttta
     tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     ggatccgtcg acctgcagcc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     ggtttgcgta ttggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaa
//