ID   PUCBM20    preliminary; circular DNA; SYN; 2722 BP.
XX
AC   IG0315; VB0021;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCbm20 or pUCPZ2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
XX
CC   NM (pUCbm20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli)
CC   CP (high)
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pUCbm21)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 remove HindIII-EcoRI 51bp 400..451,
FT                   \ MCS/2635bp
FT                   2. oligo EcoRI-HindIII 87bp, MCS
FT                   -> pUCbm20 2722bp"
FT   CDS             >1..<728
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    207..333
FT                   /note="MCS unique EcoRI-SmaI-BamHI-MluI-BfrI-NotI-
FT                   EclXI-KspI-KpnI-ApaI-SalI-XbaI-SacI-PstI-SphI-EcoRV-
FT                   NcoI-HindIII"
FT   misc_binding    482..482
FT                   /note="SIT unique NarI"
FT   misc_binding    485..485
FT                   /note="SIT unique BbeI"
FT   misc_binding    534..534
FT                   /note="SIT unique NdeI"
FT   misc_binding    728..728
FT                   /note="SIT unique DraII"
FT   misc_binding    789..789
FT                   /note="SIT unique AatII"
FT   misc_binding    903..903
FT                   /note="SIT unique SspI"
FT   CDS             988..1776
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1108..1108
FT                   /note="SIT unique Asp700I"
FT   misc_binding    1227..1227
FT                   /note="SIT unique ScaI"
FT   misc_binding    1623..1623
FT                   /note="SIT unique Cfr101I"
FT   rep_origin      complement(>2000..<2700)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2722 BP; 664 A; 698 C; 688 G; 672 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     gatccacgcg tcttaaggcg gccgcggtac cgggcccgtc gactctagag ctctgcaggc
     atgcgatatc ccatggaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     gagcccgtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     cagtgagcga ggaagcggaa ga
//