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ID   PUCBM21    preliminary; circular DNA; SYN; 2725 BP.
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AC   IG0316; VB0021;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUCbm21 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
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CC   NM (pUCbm21)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli)
CC   CP (high)
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR (pUCbm20)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 remove EcoRI-HindIII 51bp 397..448,
FT                   \ MCS/2635bp
FT                   2. oligo EcoRI-HindIII 90bp, MCS
FT                   -> pUCbm21 2725bp"
FT   CDS             >1..<728
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    207..333
FT                   /note="MCS unique HindIII-NcoI-EcoRV-SphI-PstI-SacI-
FT                   XbaI-SalI-ApaI-KpnI-KspI-NotI-EclXI-BfrI-MluI-BamHI-
FT                   SmaI-EcoRI"
FT   misc_binding    482..482
FT                   /note="SIT unique NarI"
FT   misc_binding    485..485
FT                   /note="SIT unique BbeI"
FT   misc_binding    534..534
FT                   /note="SIT unique NdeI"
FT   misc_binding    728..728
FT                   /note="SIT unique DraII"
FT   misc_binding    789..789
FT                   /note="SIT unique AatII"
FT   misc_binding    903..903
FT                   /note="SIT unique SspI"
FT   CDS             988..1776
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1108..1108
FT                   /note="SIT unique Asp700I"
FT   misc_binding    1227..1227
FT                   /note="SIT unique ScaI"
FT   misc_binding    1623..1623
FT                   /note="SIT unique Cfr101I"
FT   rep_origin      complement(>2000..<2700)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2725 BP; 666 A; 699 C; 687 G; 673 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttcc
     atgggatatc gcatgcctgc agagctctag agtcgacggg cccggtaccg cggccgcact
     taagttacgc gtggatcccc gggaattcac tggccgtcgt tttacaacgt cgtgactggg
     aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     ctggagcccg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     agtcagtgag cgaggaagcg gaaga
//