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ID   PUCF1      preliminary; circular DNA; SYN; 3200 BP.
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AC   L09144; VB0072;
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DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUC-f1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3200
RC   pUC-f1 from pUC series & f1 or phage PF1
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pUC-f1
RA   Schwarz B.;
RT   ;
RL   Submitted (00-XXX-1991) by:
RL   Schwarz B., MPI Biochemie, Martinsried.
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Citation sequence information from PL-Pharmacia. copyright.
CC   Revised 16-DEC-1986 by F. Pfeiffer:
CC   1576/7 'AT' to 'TA' to match the revised sequence of pBR322
CC   The beta-galactosidase mRNA sequence including the multiple
CC   cloning site of M13mp19 is on the strand complementary to that
CC   shown.
CC   old Pharmacia vector.
CC   NM (pUC-f1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(PF1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp, ori/amp gene
FT                   2. f1 500bp, ori
FT                   -> pUC-f1 3200bp"
FT   misc_feature    complement(1..414)
FT                   /note="from pUC19"
FT   misc_binding    complement(196..414)
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI
FT                   M13mp19/pUC19-Polylinker"
FT   misc_feature    complement(415..928)
FT                   /note="from bacteriophage f1"
FT   misc_binding    complement(931..966)
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI
FT                   M13mp19/pUC19-Polylinker"
FT   misc_feature    complement(929..1198)
FT                   /note="from pUC19"
FT   misc_feature    complement(1199..3200)
FT                   /note="from pUC19"
FT   CDS             complement(2143..2931)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3200 BP; 829 A; 786 C; 817 G; 768 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccaccccg
     gttgataatc agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta
     aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggat gccccgattt
     agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     gcgcttaatg cgccgctaca gggcgcgtgg ggatcctcta gagtcgacct gcaggcatgc
     aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     tatcacgagg ccctttcgtc
//