back Return to this vector's summary.
ID   PUCGM      preliminary; circular DNA; SYN; 3563 BP.
XX
AC   U04610;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCGM - incomplete.
XX
KW   cloning vector; beta-lactamase; gentamycin acetyl transferase;
KW   gentamycin-resistance; antibiotic resistance.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3563
RC   pJQ113 from pBluescript series & pPH1JI
RC   pUC1918 from pUC19
RC   pUCGM from pJQ113 & pUC1918
RC   pGMomega1 from pUCGM & pHP45omega
RA   Schweizer H.P.;
RT   "Small broad-host-range gentamycin resistance gene cassettes for
RT   site-specific insertion and deletion mutagenesis";
RL   Biotechniques 15:831-834(1993).
XX
RN   [2]
RP   455-1273
RC   from Tn1696; [similar to Tn21]
RC   RIP135 from Enterobacter IncL/M plasmids
RC   R1033 from Pseudomonas aeruginosa IncP1 plasmids, Tn1696 genta gene
RA   Wohlleben W., Arnold W., Bissonette L., Palletier A., Tanguay A.,
RA   Roy P.H., Gamboa G.C., Barry G.F., Aubert E., Davies J.,
RA   Kagan S.A.;
RT   "On the evolution of Tn21-like multiresistance transposons: sequence
RT   analysis of the gene (aacC(3)-1), another member of the Tn21-based
RT   expression cassette";
RL   Mol. Gen. Genet. 217:202-208(1989).
XX
RN   [3]
RP   1-3563
RC   pUCGM
RA   Schweizer H.P.;
RT   ;
RL   Submitted (17-DEC-1993) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Dr. N.W., Calgary, Alberta T2N 4N1, CANADA.
XX
RN   [4]
RC   R1033 from Pseudomonas aeruginosa IncP1 plasmids, Tn1696 genta gene
RC   Tn5-GmSpSm from R1033 & Tn5
RC   pXS1 from pAT153 & R1033
RC   pXS2 from pUC9 & R1033
RC   pVW1 from pXS1 & Tn5-GmSpSm
RC   pVW2 from pVW1 & pXS1
RC   pXS102 from Tn5-GmSpSm & pXS1
RA   Hirsch P.R., Wang C.L., Woodward M.J.;
RT   "Construction of a Tn5 derivative determining resistance to
RT   gentamycin and spectinomycin using a fragment cloned form R1033";
RL   Gene 48:203-209(1986).
XX
RN   [5]
RC   [Tn21, Tn2410, Tn2603, Tn4000, Tn1696, Tn2424 from amp transposons]
RA   Ouellette M., Roy P.H.;
RT   "Homology of ORFs from Tn2603 and from R46 to site-specific
RT   recombinases";
RL   Nucleic Acids Res. 15:10055-10055(1987).
XX
RN   [6]
RC   pBWH100 from Citrobacter diversus, aacA1 gene/amp gene/sulf gene
RC   pFTC1063 from pBR322 & pBWH100
RC   pFTC1163 from pFTC1063
RC   pFTC1164 from pFTC1063
RC   pFTC1165 from pFTC1063
RC   pFTC1266 from pFTC1063
RA   Tenover F.C., Filpula D., Phillips K.L., Plorde J.J.;
RT   "Cloning and sequencing of a gene encoding an aminoglycoside
RT   6'-N-acetyltransferase from an R factor of Citrobacter diversus";
RL   J. Bacteriol. 170:471-473(1988).
XX
RN   [7]
RC   pDGO117 from pDGO101
RC   pDGO119 from pDGO117
RA   Cameron F.H., Groot Obbink D.J., Ackerman V.P., Hall R.M.;
RT   "Nucleotide sequence of the AAD(2') aminoglycoside
RT   adenylyl-transferase determinant aadB. Evolutionary relationship
RT   of this region with those surrounding aadA in R538-1 and dhfrII in
RT   R388";
RL   Nucleic Acids Res. 14:8625-8635(1986).
XX
RN   [8]
RC   R46 or pKM101 from E.coli incN plasmid group
RC   R538-1 from Tn21
RC   pSa, R388 from E.coli incW plasmid group
RA   Hall R.M., Vockler C.;
RT   "The region of the IncN plasmid R46 coding for resistance to
RT   beta-lactam antibiotics, streptomycin/spectinomycin and
RT   sulfonamides is closely related to antibiotic resistanc segments
RT   found in IncW plasmids and in Tn21-like transposons";
RL   Nucleic Acids Res. 15:7491-7501(1987).
XX
RN   [9]
RC   pBWH100 from Citrobacter diversus, aacA1 gene/amp gene/sulf gene
RA   Mayer K.H., Hopkins J.D., Gilleece E.S., Chao L., O'Brien T.F.;
RT   "Computer assisted correlations between antibiotypes of clinical
RT   isolates and the endonuclease restriction fragment types of their
RT   plasmids";
RL   Antibiotic Resistance Transposition and Other Mech 0:41-62(1980).
RL   Mitsuhashi S., Rosival L., Krcmery V., Springer-Verlag, Berlin.
XX
RN   [10]
RC   pDGO100 from pBR322 & E.coli aadB gene
RC   pDGO101 from pBR322 & E.coli aadB gene
RC   pDGO103 from pBR322 & E.coli aadB gene
RC   pDGO109 from pBR322 & E.coli aadB gene
RA   Groot Obbink D.J., Ritchie L.J., Cameron F.H., Mattick J.S.,
RA   Ackerman V.P.;
RT   "Construction of a gentamicin resistance gene probe for
RT   epidemiological studies";
RL   Antimicrob. Agents Chemother. 28:96-102(1985).
XX
RN   [11]
RC   pPH1JI from R751 & R1033, Tn1696
RA   Hirsch P.R.;
RT   ;
RL   Thesis [University of East Anglia] 0:0-0(1978).
XX
CC   NCBI gi: 440199
CC   NM (pUCGM)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC1918 from pUC19)(pJQ113 from pBluescript & pPH1JI, from R1033)
CC   BR ()
CC   OF (pGMomega1 also from pHP45omega)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Pseudomonas IncP1 plasmids, genta gene
FT                   -> R1033
FT                   1. R1033
FT                   -> pPH1JI
FT                   1. pPH1JI BamHI-HindIII 2400bp, genta gene
FT                   2. pBluescript remove BamHI-HindIII 30bp 445..475,
FT                   \ MCS/2716bp
FT                   -> pJQ113 5100bp
FT                   1. pUC18 NdeI-EcoRI 266bp 185..451,
FT                   \ MCS/lacZ [264bp]
FT                   2. pUC19 remove NdeI-EcoRI 212bp 185..397,
FT                   \ MCS/lacZ/2474bp
FT                   -> pUC1918 2740bp
FT                   1. pJQ113 HindIII-NotI 819bp, genta gene
FT                   blunt end
FT                   2. pUC1918 EcoRI 5932bp 451..451
FT                   blunt end
FT                   -> pUCGM 3563bp"
FT   misc_feature    1..454
FT                   /note="from pUC1918"
FT   misc_binding    399..449
FT                   /note="MCS, from pUC1918"
FT   -35_signal      453..458
FT                   /note="S35 E. coli aacC1 gene, from Tn1696"
FT   misc_feature    455..1273
FT                   /note="from Tn1696"
FT   -10_signal      476..481
FT                   /note="S10 E. coli aacC1 gene, from Tn1696"
FT   RBS             658..661
FT                   /note="RBS E. coli aacC1 gene, from Tn1696"
FT   CDS             670..1203
FT                   /note="ANT E. coli gentamycin acetyltransferase-3-1
FT                   gene (aacC1); gentamycin resistance gene (gen);
FT                   NCBI gi: 440200, from Tn1696"
FT   misc_feature    1274..3563
FT                   /note="from pUC1918"
FT   misc_binding    1279..1329
FT                   /note="MCS, from pUC1918"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2503..3363)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp);
FT                   NCBI gi: 440201, from pUC1918"
FT   RBS             3369..3372
FT                   /note="RBS E. coli bla gene, from pUC1918"
XX
SQ   Sequence 3563 BP; 858 A; 914 C; 925 G; 866 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccccgggt accgagctcg aattgacata agcctgttcg gttcgtaaac
     tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta
     tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc
     aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac
     tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc
     gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
     aggtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac
     cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
     ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
     acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
     taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca
     caacgccgcg gccaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     gcgtatcacg aggccctttc gtc
//