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ID   PUCHSNEO   preliminary; circular DNA; INV; 5073 BP.
XX
AC   X01803;
XX
DT   07-NOV-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Drosophila/E.coli plasmid vector pUChsneo - complete.
XX
KW   cloning vector.
XX
OS   Drosophila melanogaster (fruit fly)
OC   Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
OC   Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
XX
RN   [1]
RP   1-5073
RC   cosP from p6.1 & pUC8 & pcos4EMBL & Carnegie 1
RC   Ca4hsneo from Carnegie 4 & hsp70-neo
RC   cosPneo from cosP & Ca4hsneo
RC   pUChsneo from pUC8 & hsp70-neo
RA   Steller H., Pirrotta V.;
RT   "A transposable P vector that confers selectable G418 resistance
RT   to Drosophila larvae";
RL   EMBO J. 4:167-171(1985).
XX
RN   [2]
RC   pUChsneo from pUC8 & hsp70-neo
RA   Pirrotta V.;
RT   ;
RL   Unpublished (1986).
XX
RN   [3]
RC   ppi25wc from ppi25
RA   Rubin G.;
RT   ;
RL   Unpublished (1985).
XX
RN   [4]
RC   pG13 from pBR322 & Drosophila hsp70 gene
RC   from pBH1
RC   from pB8
RA   Ingolia T.D., Craig E.A., McCarthy B.J.;
RT   "Sequence of three copies of the gene for the major Drosophila
RT   heat shock induced protein and their flanking regions";
RL   Cell 21:669-679(1980).
XX
CC   NM (pUChsneo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (D.melanogaster larvae)(Drosophila)
CC   CP ()
CC   FN (transposable element)
CC   SE ()
CC   PA (pUC8)(hsp70-neo)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Drosophila
FT                   -> p6.1
FT                   1. pUC8 NarI 2665bp 426..426
FT                   2. p6.1 SalI-XmnI 538bp, +538 5' P element
FT                   BAL31 nuclease:
FT                   -> plasmid 3200bp
FT                   1. Tn5 BglII-BamHI 1541bp 1516..3057,
FT                   \ neo gene coding region only
FT                   2. hsp70-neo gene XbaI-XmnI 456bp, promoter/leader
FT                   BamHI linker 6bp ggatcc:
FT                   -> fragment 1630
FT                   1. fragment BamHI-SmaI 175bp, 3' end neo
FT                   \ pSV2-neo 1599..1774/complement
FT                   -> fragment2 175bp
FT                   1. pUC8 SalI 2665bp 247..247
FT                   2. p6.1 XhoI-SalI, 3' P element
FT                   :BAL31 nuclease
FT                   -> plasmid2
FT                   1. Carnegie 1 3616bp
FT                   2. plasmid
FT                   -> plasmid3
FT                   1. pBR322 PvuII 4361bp 2069..2069
FT                   2. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   3. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   -> pcos4EMBL 6300bp [2 tandem cos with PvuII between]
FT                   1. plasmid2 BamHI-SalI, 3' P element
FT                   2. pcos4EMBL PvuI-SalI 4700bp, lambda cos/ori/5' amp
FT                   3. plasmid3 PvuI-BamHI 1500bp, amp/5' P/MCS
FT                   -> pUChsneo 5073bp"
FT   transposon      1..542
FT                   /note="TRN Drosophila P element fragment"
FT   repeat_unit     1..31
FT                   /note="RPT inverted repeat"
FT   misc_feature    543..3208
FT                   /note="from pUC8"
FT   misc_binding    705..740
FT                   /note="MCS"
FT   promoter        3221..3672
FT                   /note="PRO Drosophila heat shock protein 70 (hsp70)
FT                   gene"
FT   CDS             3676..4678
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   transposon      4685..5073
FT                   /note="TRN Drosophila P element fragment"
FT   repeat_unit     5044..5073
FT                   /note="RPT inverted repeat"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5073 BP; 1317 A; 1222 C; 1287 G; 1247 T; 0 other;
     catgatgaaa taacataagg tggtcccgtc gaaagccgaa gcttaccgaa gtatacactt
     aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
     aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
     ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
     agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
     attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
     cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
     ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
     ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
     aacgccattc gccattcagg ctacgcaact gttgggaagg gcgatcggtg cgggcctctt
     cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt tgggtaacgc
     cagggttttc ccagtcagta cgttgtaaaa cgacggccag tgccaagctt ggctgcaggt
     cgacggatcc ccgggaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     aacctgtcgt gccagctgga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttag
     ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     gcgtaaggag aaaataccgc atcaggcgaa ttaattcccc tagaatccca aaacaaactg
     gttattgtgg taggtcattt gtttggcaga agaaaactcg agaaatttct ctggccgtta
     ttcgttattc tctcttttct ttttgggtct ccctctctgc actaatgctc tctcactctg
     tcacacagta aacggcatac tgctctcgtt ggttcgagag agcgcgcctc gaatgttcgc
     gaaaagagcg ccggagtata aatagagcgc ttcgtctacg gagcgacaat tcaattcaaa
     caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     gaactctgaa taggggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     tccgtcgagc gtataaccat ctgtacaaaa aaggatttcc tttgcccagt cgtacgactt
     tgtacagatg gttatcagat gtggacataa aaagaggatg tttggatgtg gtcatagacc
     taatggacag tgatggagtt gatgacgccg acaagctttg cgtactcgct caaattatta
     aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac
     ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac
     tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt
     gccgacggga ccaccttatg ttatttcatc atg
//