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ID PUCHSNEO preliminary; circular DNA; INV; 5073 BP.
XX
AC X01803;
XX
DT 07-NOV-1985 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Drosophila/E.coli plasmid vector pUChsneo - complete.
XX
KW cloning vector.
XX
OS Drosophila melanogaster (fruit fly)
OC Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
OC Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
OC Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
XX
RN [1]
RP 1-5073
RC cosP from p6.1 & pUC8 & pcos4EMBL & Carnegie 1
RC Ca4hsneo from Carnegie 4 & hsp70-neo
RC cosPneo from cosP & Ca4hsneo
RC pUChsneo from pUC8 & hsp70-neo
RA Steller H., Pirrotta V.;
RT "A transposable P vector that confers selectable G418 resistance
RT to Drosophila larvae";
RL EMBO J. 4:167-171(1985).
XX
RN [2]
RC pUChsneo from pUC8 & hsp70-neo
RA Pirrotta V.;
RT ;
RL Unpublished (1986).
XX
RN [3]
RC ppi25wc from ppi25
RA Rubin G.;
RT ;
RL Unpublished (1985).
XX
RN [4]
RC pG13 from pBR322 & Drosophila hsp70 gene
RC from pBH1
RC from pB8
RA Ingolia T.D., Craig E.A., McCarthy B.J.;
RT "Sequence of three copies of the gene for the major Drosophila
RT heat shock induced protein and their flanking regions";
RL Cell 21:669-679(1980).
XX
CC NM (pUChsneo)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (D.melanogaster larvae)(Drosophila)
CC CP ()
CC FN (transposable element)
CC SE ()
CC PA (pUC8)(hsp70-neo)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Drosophila
FT -> p6.1
FT 1. pUC8 NarI 2665bp 426..426
FT 2. p6.1 SalI-XmnI 538bp, +538 5' P element
FT BAL31 nuclease:
FT -> plasmid 3200bp
FT 1. Tn5 BglII-BamHI 1541bp 1516..3057,
FT \ neo gene coding region only
FT 2. hsp70-neo gene XbaI-XmnI 456bp, promoter/leader
FT BamHI linker 6bp ggatcc:
FT -> fragment 1630
FT 1. fragment BamHI-SmaI 175bp, 3' end neo
FT \ pSV2-neo 1599..1774/complement
FT -> fragment2 175bp
FT 1. pUC8 SalI 2665bp 247..247
FT 2. p6.1 XhoI-SalI, 3' P element
FT :BAL31 nuclease
FT -> plasmid2
FT 1. Carnegie 1 3616bp
FT 2. plasmid
FT -> plasmid3
FT 1. pBR322 PvuII 4361bp 2069..2069
FT 2. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT 3. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT -> pcos4EMBL 6300bp [2 tandem cos with PvuII between]
FT 1. plasmid2 BamHI-SalI, 3' P element
FT 2. pcos4EMBL PvuI-SalI 4700bp, lambda cos/ori/5' amp
FT 3. plasmid3 PvuI-BamHI 1500bp, amp/5' P/MCS
FT -> pUChsneo 5073bp"
FT transposon 1..542
FT /note="TRN Drosophila P element fragment"
FT repeat_unit 1..31
FT /note="RPT inverted repeat"
FT misc_feature 543..3208
FT /note="from pUC8"
FT misc_binding 705..740
FT /note="MCS"
FT promoter 3221..3672
FT /note="PRO Drosophila heat shock protein 70 (hsp70)
FT gene"
FT CDS 3676..4678
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)"
FT transposon 4685..5073
FT /note="TRN Drosophila P element fragment"
FT repeat_unit 5044..5073
FT /note="RPT inverted repeat"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 5073 BP; 1317 A; 1222 C; 1287 G; 1247 T; 0 other;
catgatgaaa taacataagg tggtcccgtc gaaagccgaa gcttaccgaa gtatacactt
aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
aacgccattc gccattcagg ctacgcaact gttgggaagg gcgatcggtg cgggcctctt
cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt tgggtaacgc
cagggttttc ccagtcagta cgttgtaaaa cgacggccag tgccaagctt ggctgcaggt
cgacggatcc ccgggaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
aacctgtcgt gccagctgga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttag
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
gcgtaaggag aaaataccgc atcaggcgaa ttaattcccc tagaatccca aaacaaactg
gttattgtgg taggtcattt gtttggcaga agaaaactcg agaaatttct ctggccgtta
ttcgttattc tctcttttct ttttgggtct ccctctctgc actaatgctc tctcactctg
tcacacagta aacggcatac tgctctcgtt ggttcgagag agcgcgcctc gaatgttcgc
gaaaagagcg ccggagtata aatagagcgc ttcgtctacg gagcgacaat tcaattcaaa
caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
gaactctgaa taggggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
tccgtcgagc gtataaccat ctgtacaaaa aaggatttcc tttgcccagt cgtacgactt
tgtacagatg gttatcagat gtggacataa aaagaggatg tttggatgtg gtcatagacc
taatggacag tgatggagtt gatgacgccg acaagctttg cgtactcgct caaattatta
aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac
ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac
tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt
gccgacggga ccaccttatg ttatttcatc atg
//