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ID   PUCP18     preliminary; circular DNA; SYN; 4557 BP.
XX
AC   U07164;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCP18 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   239-2122
RC   pUCP18 from pUC18
RC   pUCP20 from pUC18
RC   pUCP22 from pUC18
RC   pUCP24 from pUC18
RC   pUCP26 from pUC18
RA   West S.E., Schweizer H.P., Dall C., Sample A.K., Runyen-Janecky L.J.;
RT   "Construction of improved Escherichia-Pseudomonas shuttle vectors
RT   derived from pUC18/19 and sequence of the region required for
RT   their replication in Pseudomonas aeruginosa";
RL   Gene 148:81-86(1994).
XX
RN   [2]
RP   1-4557
RC   pUCP19 from pUC19 & pUC181.8
RC   pUCP18 from pUCP19 & pUC18
RA   Schweizer H.;
RT   "Escherichia-Pseudomonas shuttle vectors derived from pUC18/19";
RL   Gene 97:109-112(1991).
XX
RN   [3]
RP   239-2122
RC   pRO1600, pRO1601 from P.aeruginosa strain PAO plasmid RP1
RC   pRO1613 from pRO1601 & pBR322
RC   pRO1614 from pRO1613 & pBR322
RA   Olsen R.H., DeBusscher G., McCombie W.R.;
RT   "Development of broad-host-range vectors and gene banks: self-cloning
RT   of the Pseudomonas aeruginosa PAO chromosome";
RL   J. Bacteriol. 150:60-69(1982).
XX
RN   [4]
RC   pUCP18
RA   Schweizer H.P.;
RT   ;
RL   Submitted (24-FEB-1994) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Dr. N.W., Calgary, Alberta, T2N 4N1, Canada.
XX
RN   [5]
RC   pUC181.8 from pUC18 & pRO1614
RA   Frank D.W., Storey D.G., Hindahl M.S., Iglewski B.H.;
RT   "Differential regulation by iron of regA and toxA transcript
RT   accumulation in Pseudomonas aeruginosa";
RL   J. Bacteriol. 171:5304-5313(1989).
XX
CC   NCBI gi: 460933
CC   NM (pUCP18)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pUCP20)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. P.aeruginosa strain PAO
FT                   -> RP1
FT                   1. RP1
FT                   mutation
FT                   -> pRO1600
FT                   1. pRO1600, carb gene
FT                   2. Tn1
FT                   -> pRO1601
FT                   1. pRO1601, carb gene
FT                   -> pRO1613
FT                   1. pRO1613 PstI-PstI 1800bp, amp/pRO1601 ori
FT                   2. pBR322 PstI 4361bp 3612..3612
FT                   -> pRO1614 6160bp
FT                   1. pUC18 PstI 2686bp 416..416, MCS
FT                   2. pRO1614 PstI-PstI 1800bp, pRO1601 ori
FT                   -> pUC181.8 4490bp
FT                   1. pUC19 NarI 2686bp 237..237
FT                   T4 DNA polymerase
FT                   2. pUC181.8 PstI-PstI 1800bp, pRO1601 ori
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pUCP19 4490bp
FT                   1. pUCP19 PvuI-PvuI 2750bp, pRO1601 ori
FT                   2. pUC18 PvuI-PvuI 1790bp 280..2070
FT                   -> pUCP18 4557bp"
FT   misc_feature    1..238
FT                   /note="from pUC18 DNA [3]"
FT   misc_feature    239..2122
FT                   /note="pRO1614 DNA [4]"
FT   RBS             1242..1247
FT                   /note="E. coli rep"
FT   CDS             1256..2089
FT                   /note="GEN E. coli replication protein"
FT   CDS             complement(2086..2340)
FT                   /note="GEN E. coli lacZ alpha"
FT   misc_feature    2123..4557
FT                   /note="from pUC18 DNA [3]"
FT   misc_binding    2270..2326
FT                   /note="MCS"
FT   RBS             2348..2351
FT                   /note="E. coli lacZ alpha"
FT   CDS             complement(3497..4357)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   RBS             4363..4366
FT                   /note="E. coli bla"
XX
SQ   Sequence 4557 BP; 1113 A; 1155 C; 1220 G; 1069 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggat ctcgcggtat
     cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     cccttaacgt gagttttcgt tccactgagc gtcagacccc aattacacgc cactggctgt
     gcttgctggg gtgacggtgg caacggtggc ggccttgctg ggctatcgcg ttggaaagaa
     acgagggaaa ggggactgat aaaccggtct tagcccctcc ccttggtgtc caaccgctct
     gtaggcctct caggcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga
     taccctgatt actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg
     gattgcccag acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac
     gtagtggtgc gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag
     gacgtctaca ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc
     cattaaggga cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt
     tgttgtattg tgggacgcag atacagtgtc cccttataca caaggaatgt cgaacgtggc
     ctcaccccca atggtttaca aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt
     tcaggaacag cggatcgttc tggcctgtat tagccaggtg aagaggagcg agcctgtcac
     cgatgaagtg atgtattcag tgacggcgga ggacatagcg acgatggcgg gtgtccctat
     cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg
     gttaacccaa gagcccaatg gcaaggggaa aagaccgagt gtgatgatta ccggctgggt
     gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat
     gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc
     caagatggac agcacccacg cgatcaggct ttacgagctg ctcatgcaat gggacagcat
     cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg tttcaactgg aaggccggta
     tccctcgatc aaggacttca agttgcgagt gcttgatcca gccgtgacgc agatcaacga
     gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc gggcgcaagg tcacacatct
     gttgttcagt tttggaccga agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg
     caaggccggg aagatttcag atgctgagat cgcgaaacag gctcgccctg gtgagacatg
     ggaagcggcc cgcgctcgac taacccagat gccgctggat ctggcctaga ggccgtggcc
     accacggccc ggcctgcctt tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
     cctgcaggtc gactctagag gatccccggg taccgagctc gaattcgtaa tcatggtcat
     agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//