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ID   PUCP20     preliminary; circular DNA; SYN; 3898 BP.
XX
AC   U07165;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCP20 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   187-1463
RC   pUCP18 from pUC18
RC   pUCP20 from pUC18
RC   pUCP22 from pUC18
RC   pUCP24 from pUC18
RC   pUCP26 from pUC18
RA   West S.E., Schweizer H.P., Dall C., Sample A.K., Runyen-Janecky L.J.;
RT   "Construction of improved Escherichia-Pseudomonas shuttle vectors
RT   derived from pUC18/19 and sequence of the region required for
RT   their replication in Pseudomonas aeruginosa";
RL   Gene 148:81-86(1994).
XX
RN   [2]
RC   pUCP20
RA   Schweizer H.P.;
RT   ;
RL   Submitted (24-FEB-1994) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Dr. N.W., Calgary, Alberta, T2N 4N1, Canada.
XX
CC   NCBI gi: 460937
CC   NM (pUCP20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pUCP18)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp, ori/lacZ
FT                   2. pRO1614 1200bp, pRO1601 ori
FT                   -> pUCP20 3898bp"
FT   misc_feature    1..186
FT                   /note="from pUC18 DNA [3]"
FT   misc_feature    187..1463
FT                   /note="pRO1614 DNA [4]"
FT   RBS             583..588
FT                   /note="E. coli rep"
FT   CDS             597..1430
FT                   /note="GEN E. coli replication protein"
FT   CDS             complement(1427..1681)
FT                   /note="GEN E. coli lacZ alpha"
FT   misc_feature    1464..3898
FT                   /note="from pUC18 DNA [3]"
FT   misc_binding    1611..1667
FT                   /note="MCS"
FT   RBS             1689..1692
FT                   /note="E. coli lacZ alpha"
FT   CDS             complement(2838..3698)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   RBS             3704..3707
FT                   /note="E. coli bla"
XX
SQ   Sequence 3898 BP; 943 A; 1004 C; 1047 G; 904 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
     ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
     ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
     cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
     ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
     ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
     ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
     cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
     ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
     ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
     tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
     ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
     tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
     tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
     ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
     tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
     atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
     agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
     tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
     gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
     gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
     caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgta atcatggtca
     tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//