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ID   PUCP22     preliminary; circular DNA; SYN; 4729 BP.
XX
AC   U07166;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCP22 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1018-2294
RC   pUCP18 from pUC18
RC   pUCP20 from pUC18
RC   pUCP22 from pUC18
RC   pUCP24 from pUC18
RC   pUCP26 from pUC18
RA   West S.E., Schweizer H.P., Dall C., Sample A.K., Runyen-Janecky L.J.;
RT   "Construction of improved Escherichia-Pseudomonas shuttle vectors
RT   derived from pUC18/19 and sequence of the region required for
RT   their replication in Pseudomonas aeruginosa";
RL   Gene 148:81-86(1994).
XX
RN   [2]
RP   1-4729
RC   pUCP22
RA   Schweizer H.P.;
RT   ;
RL   Submitted (24-FEB-1994) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Dr. N.W., Calgary, Alberta, T2N 4N1, Canada.
XX
CC   NCBI gi: 460941
CC   NM (pUCP22)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pUCP18)(pUCP20)(pUCP24)(pUCP26)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. P.aeruginosa strain PAO
FT                   -> RP1
FT                   1. RP1
FT                   mutation
FT                   -> pRO1600
FT                   1. pRO1600, carb gene
FT                   2. Tn1
FT                   -> pRO1601
FT                   1. pRO1601, carb gene
FT                   -> pRO1613
FT                   1. pRO1613 PstI-PstI 1800bp, amp/pRO1601 ori
FT                   2. pBR322 PstI 4361bp 3612..3612
FT                   -> pRO1614 6160bp
FT                   1. pUC18 2686bp, ori/lacZ
FT                   2. pRO1614 1200bp, pRO1601 ori
FT                   3. pUCGM 800bp, pRO1601 ori
FT                   -> pUCP22 4729bp"
FT   misc_feature    1..186
FT                   /note="from pUC18 DNA [2]"
FT   misc_feature    187..1017
FT                   /note="from pUCGM DNA [5]"
FT   CDS             complement(263..796)
FT                   /note="ANT E. coli gentamycin acetyltransferase-3-1
FT                   gene aacC1 [5]"
FT   misc_feature    1018..2294
FT                   /note="pRO1614 DNA [3]"
FT   RBS             1414..1419
FT                   /note="E. coli rep"
FT   CDS             1428..2261
FT                   /note="GEN E. coli replication protein"
FT   CDS             complement(2258..2512)
FT                   /note="GEN E. coli lacZ alpha"
FT   misc_feature    2295..4729
FT                   /note="from pUC18 DNA [2]"
FT   misc_binding    2442..2498
FT                   /note="MCS"
FT   RBS             2520..2523
FT                   /note="E. coli lacZ alpha"
FT   CDS             complement(3669..4529)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   RBS             4535..4538
FT                   /note="E. coli bla"
XX
SQ   Sequence 4729 BP; 1142 A; 1229 C; 1270 G; 1088 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatacgaa ttggccgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc
     cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
     cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
     cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga
     gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga
     tctcactacg cggctgctca aacctgggca gaacgtaagc cgcgagagcg ccaacaaccg
     cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
     aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
     gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
     tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
     tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caattcgcct
     ctcaggcgcc gctggtgccg ctggttggac gccaagggtg aatccgcctc gataccctga
     ttactcgctt cctgcgccct ctcaggcggc gataggggac tggtaaaacg gggattgccc
     agacgcctcc cccgcccctt caggggcaca aatgcggccc caacggggcc acgtagtggt
     gcgttttttg cgtttccacc cttttcttcc ttttcccttt taaacctttt aggacgtcta
     caggccacgt aatccgtggc ctgtagagtt taaaaaggga cggatttgtt gccattaagg
     gacggatttg ttgttaagaa gggacggatt tgttgttgta aagggacgga tttgttgtat
     tgtgggacgc agatacagtg tccccttata cacaaggaat gtcgaacgtg gcctcacccc
     caatggttta caaaagcaat gccctggtcg aggccgcgta tcgcctcagt gttcaggaac
     agcggatcgt tctggcctgt attagccagg tgaagaggag cgagcctgtc accgatgaag
     tgatgtattc agtgacggcg gaggacatag cgacgatggc gggtgtccct atcgaatctt
     cctacaacca gctcaaagaa gcggccctgc gcctgaaacg gcgggaagtc cggttaaccc
     aagagcccaa tggcaagggg aaaagaccga gtgtgatgat taccggctgg gtgcaaacaa
     tcatctaccg ggagggtgag ggccgtgtag aactcaggtt caccaaagac atgctgccgt
     acctgacgga actcaccaaa cagttcacca aatacgcctt ggctgacgtg gccaagatgg
     acagcaccca cgcgatcagg ctttacgagc tgctcatgca atgggacagc atcggccagc
     gcgaaataga aattgaccag ctgcgaaagt ggtttcaact ggaaggccgg tatccctcga
     tcaaggactt caagttgcga gtgcttgatc cagccgtgac gcagatcaac gagcacagcc
     cgctacaggt ggagtgggcg cagcgaaaga ccgggcgcaa ggtcacacat ctgttgttca
     gttttggacc gaagaagccc gccaaggcgg tgggtaaggc cccagcgaag cgcaaggccg
     ggaagatttc agatgctgag atcgcgaaac aggctcgccc tggtgagaca tgggaagcgg
     cccgcgctcg actaacccag atgccgctgg atctggccta gaggccgtgg ccaccacggc
     ccggcctgcc tttcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg
     tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatggtc atagctgttt
     cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//