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ID   PUCP26     preliminary; circular DNA; SYN; 4977 BP.
XX
AC   U07168;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pUCP26 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-1277
RC   pUCP18 from pUC18
RC   pUCP20 from pUC18
RC   pUCP22 from pUC18
RC   pUCP24 from pUC18
RC   pUCP26 from pUC18
RA   West S.E., Schweizer H.P., Dall C., Sample A.K., Runyen-Janecky L.J.;
RT   "Construction of improved Escherichia-Pseudomonas shuttle vectors
RT   derived from pUC18/19 and sequence of the region required for
RT   their replication in Pseudomonas aeruginosa";
RL   Gene 148:81-86(1994).
XX
RN   [2]
RP   3206-4977
RC   pBR322ZF from pBR322 & pGEM-3Zf+
RC   pBR322F1 from pBR322 & pGEM-3Zf+
RC   plasmid from pBR322F1 & oligo
RC   pSELECT-1 from pBR322ZF & plasmid
RA   Lewis M.K., Thompson D.V.;
RT   "Efficient site-directed in vitro mutagenesis using ampicillin
RT   selection";
RL   Nucleic Acids Res. 18:3439-3443(1990).
XX
RN   [3]
RP   1-4977
RC   pUCP26
RA   Schweizer H.P.;
RT   ;
RL   Submitted (24-FEB-1994) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Dr. N.W., Calgary, Alberta, T2N 4N1, Canada.
XX
CC   NCBI gi: 460950
CC   NM (pUCP26)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pUCP18)(pUCP20)(pUCP22)(pUCP24)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. P.aeruginosa strain PAO
FT                   -> RP1
FT                   1. RP1
FT                   mutation
FT                   -> pRO1600
FT                   1. pRO1600, carb gene
FT                   2. Tn1
FT                   -> pRO1601
FT                   1. pRO1601, carb gene
FT                   -> pRO1613
FT                   1. pRO1613 PstI-PstI 1800bp, amp/pRO1601 ori
FT                   2. pBR322 PstI 4361bp 3612..3612
FT                   -> pRO1614 6160bp
FT                   1. pUC18 2686bp, ori/lacZ
FT                   2. pRO1614 1200bp, pRO1601 ori
FT                   3. pUCGM 800bp, pRO1601 ori
FT                   -> pUCP26 4977bp"
FT   misc_feature    1..1277
FT                   /note="pRO1614 DNA [3]"
FT   RBS             398..402
FT                   /note="E. coli rep"
FT   CDS             411..1244
FT                   /note="GEN E. coli replication protein"
FT   CDS             complement(1241..1495)
FT                   /note="GEN E. coli lacZ alpha"
FT   misc_feature    1278..3205
FT                   /note="from pUC18 DNA [2]"
FT   misc_binding    1425..1481
FT                   /note="MCS"
FT   RBS             1503..1506
FT                   /note="E. coli lacZ alpha"
FT   misc_feature    3206..4036
FT                   /note="from pUCGM DNA [5]"
FT   CDS             complement(3303..4493)
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   RBS             4500..4504
FT                   /gene="RBS E. coli tet gene"
FT   misc_feature    3206..4977
FT                   /note="from pSELECT-1 DNA [4]"
XX
SQ   Sequence 4977 BP; 1164 A; 1340 C; 1403 G; 1070 T; 0 other;
     cctctcaggc gccgctggtg ccgctggttg gacgccaagg gtgaatccgc ctcgataccc
     tgattactcg cttcctgcgc cctctcaggc ggcgataggg gactggtaaa acggggattg
     cccagacgcc tcccccgccc cttcaggggc acaaatgcgg ccccaacggg gccacgtagt
     ggtgcgtttt ttgcgtttcc acccttttct tccttttccc ttttaaacct tttaggacgt
     ctacaggcca cgtaatccgt ggcctgtaga gtttaaaaag ggacggattt gttgccatta
     agggacggat ttgttgttaa gaagggacgg atttgttgtt gtaaagggac ggatttgttg
     tattgtggga cgcagataca gtgtcccctt atacacaagg aatgtcgaac gtggcctcac
     ccccaatggt ttacaaaagc aatgccctgg tcgaggccgc gtatcgcctc agtgttcagg
     aacagcggat cgttctggcc tgtattagcc aggtgaagag gagcgagcct gtcaccgatg
     aagtgatgta ttcagtgacg gcggaggaca tagcgacgat ggcgggtgtc cctatcgaat
     cttcctacaa ccagctcaaa gaagcggccc tgcgcctgaa acggcgggaa gtccggttaa
     cccaagagcc caatggcaag gggaaaagac cgagtgtgat gattaccggc tgggtgcaaa
     caatcatcta ccgggagggt gagggccgtg tagaactcag gttcaccaaa gacatgctgc
     cgtacctgac ggaactcacc aaacagttca ccaaatacgc cttggctgac gtggccaaga
     tggacagcac ccacgcgatc aggctttacg agctgctcat gcaatgggac agcatcggcc
     agcgcgaaat agaaattgac cagctgcgaa agtggtttca actggaaggc cggtatccct
     cgatcaagga cttcaagttg cgagtgcttg atccagccgt gacgcagatc aacgagcaca
     gcccgctaca ggtggagtgg gcgcagcgaa agaccgggcg caaggtcaca catctgttgt
     tcagttttgg accgaagaag cccgccaagg cggtgggtaa ggccccagcg aagcgcaagg
     ccgggaagat ttcagatgct gagatcgcga aacaggctcg ccctggtgag acatgggaag
     cggcccgcgc tcgactaacc cagatgccgc tggatctggc ctagaggccg tggccaccac
     ggcccggcct gcctttcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
     aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg
     tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtcaagg gttggtttgc gcattcacag ttctccgcaa
     gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
     attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
     tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
     atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat
     ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
     tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     ttgaaggctc tcaagggcat cggtcggcgc tctcccttat gcgactcctg cattaggaag
     cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgtaag
     gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagaga
     atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta atcgatgata
     agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg attctgtgga
     taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagct
//