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ID   PUCSP    preliminary; circular DNA; SYN; 2909 BP.
XX
AC   IG9924;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pUCSP - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUCSP from pUC12 & pSP64, Sp6 promoter
RC   pUCT7 from pUC12 & pT7-2, T7 promoter
RC   Lorus from Loric & pUCSP
RC   Lordel from Lorus
RC   LordelO from Lordel
RC   LordelB from LordelO
RC   LdBp12 from LordelB & pUC12
RC   Loris from LdBp12
RC   pUCT7Bdelta from pUCT7
RC   LorspT7 from Loris & pUCT7Bdelta
RC   LoristB from LorspT7
RA   Cross S.H., Little P.F.;
RT   "A cosmid vector for systematic chromosome walking";
RL   Gene 49:9-22(1986).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUCSP)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC12)(pSP64)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT                   \ MCS/2653bp
FT                   2. pSP64 Sau3AI/MboI-HindIII 256bp 2751..2999..8,
FT                   \ Sp6 promoter
FT                   -> pUCSP 2909bp"
FT   -               1..248
FT                   /note="pUC12 1..248 248bp
FT                   BamHI =        G^GATCC
FT                   MboI = Sau3AI = ^GATC"
FT   -               249..504
FT                   /note="pSP64 2751..2999..7 256bp
FT                   HindIII = A^AGCTT"
FT   -               505..2909
FT                   /note="pUC12 276..2680 2405bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2909 BP; 725 A; 732 C; 728 G; 724 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     cgcccgggga tctggctagc gatgaccctg ctgattggtt cgctgaccat ttccggggtg
     cggaacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt
     ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta
     catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     atttaggtga cactatagaa tacaagcttg gcactggccg tcgttttaca acgtcgtgac
     tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     agcgagtcag tgagcgagga agcggaaga
//