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ID   PUCT7      preliminary; circular DNA; SYN; 2714 BP.
XX
AC   IG9927;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pUCT7 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUCSP from pUC12 & pSP64, Sp6 promoter
RC   pUCT7 from pUC12 & pT7-2, T7 promoter
RC   Lorus from Loric & pUCSP
RC   Lordel from Lorus
RC   LordelO from Lordel
RC   LordelB from LordelO
RC   LdBp12 from LordelB & pUC12
RC   Loris from LdBp12
RC   pUCT7Bdelta from pUCT7
RC   LorspT7 from Loris & pUCT7Bdelta
RC   LoristB from LorspT7
RA   Cross S.H., Little P.F.;
RT   "A cosmid vector for systematic chromosome walking";
RL   Gene 49:9-22(1986).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUCT7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC12)(pT7-2)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove HindIII-HaeII 168bp 276..444,
FT                   \ MCS/2512bp
FT                   2. pT7-2 HindIII-HaeII 202bp 239..441, T7 promoter
FT                   -> pUCT7 2714bp"
FT   -               1..275
FT                   /note="pUC12 1..275 275bp
FT                   HindIII = A^AGCT T"
FT   -               276..477
FT                   /note="pT7-2 239..440 202bp
FT                   HaeII = RGCGC^Y"
FT   -               478..2714
FT                   /note="pUC12 444..2680 2237bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2714 BP; 663 A; 683 C; 696 G; 672 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     cgcccgggga tcctctagag tcgacctgca gcccacattc gccattcagg ctacgcaact
     gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaggggggat
     gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     cgacggccag tgaattcgag ctcgcccggg gatcctctag agtcgacctg cagcccactg
     atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     gaggaagcgg aaga
//