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ID   PUCTAG     preliminary; circular DNA; SYN; 5869 BP.
XX
AC   ATCC37460;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pUCTag - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSVXB-p1 from SV40 ori/Tantigen & pUC18 & pBR327
RC   pSVXBenh from pSVXB-p1 & pbetaGXSV512, SV40 enhancer
RC   pSVXB1504, pSVXB912, pSVXB490, pSVXB276 from pSVXB-p1 & HBV
RC   pUC18 HBV912 from pUC18 & HBV
RC   pHB23, pHB31 from pSVXB-p1 & pUC18 HBV912
RC   [pUCTag from pUC18 & pSVXB-p1]
RA   Tognoni A., Cattaneo R., Serfling E., Schaffner W.;
RT   "A novel expression selection approach allows precise mapping of
RT   the hepatitis B virus enhancer";
RL   Nucleic Acids Res. 13:7457-7472(1985).
XX
RN   [2]
RC   p24beta from SV40 & rabbit beta-globin gene
RC   from p24beta & M23
RC   pbetaGX from SV40 enhancer
RC   pbetaGXSV512 from SV40 enhancer
RC   [pbetaX14 from pbetaGX & linker]
RC   pDept from SV40 enhancer
RC   pTAR7 from Ig enhancer
RA   Banerji J., Olson L., Schaffner W.;
RT   "A lymphocyte-specific cellular enhancer is located downstream of
RT   the joining region in immunoglobulin heavy chain genes";
RL   Cell 33:729-740(1983).
XX
RN   [3]
RC   M23 from mouse Ig heavy chain gene
RA   Marcu K.B., Banerji J., Penncavage N.A., Lang R., Arnheim N.;
RT   "5' flanking region of immunoglobulin heavy chain constant region
RT   genes displays length heterogeneity in germlines of inbred mouse
RT   strains";
RL   Cell 22:187-196(1980).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   The SV40 T-antigen gene and origin of replication from pSVXB-p1 was
CC   cloned as an AvaI/XbaI fragment into XbaI/HincII cut pUC18, after
CC   filling in the AvaI site.
CC   The SV40 T antigen gene was cloned into pUC18.
CC   A shuttle vector for cloning sequences which function in mammalian
CC   cells as enhancers.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUCTag)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (SV40)(pBR327)(pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. SV40 BamHI-EcoRI 4492bp 2534..5243..1783,
FT                   \ ori/T ant
FT                   fill in:
FT                   2. pBR327 AvaI-EcoRI 1847bp 1425..3272, ori/amp gene
FT                   fill in:
FT                   -> pSVXB-p1 6339bp [AvaI, no BamHI]
FT                   1. pUC18 remove HincII-XbaI 4bp 420..424, MCS/2682bp
FT                   :fill in
FT                   2. pSVXB-p1 AvaI-HincII 3187bp (2531)..2534..5243..473
FT                   \ ori/Tantigen
FT                   fill in:
FT                   -> pUCTag 5869bp"
FT   -               1..419
FT                   /note="pUC18 1..419 419bp
FT                   HindII = HincII = GTY^RAC"
FT   -               420..3602
FT                   /note="SV40 2534..5243..473 3183bp complement
FT                   BamHI = G^GATC C
FT                   AvaI =       C^YCGRG
FT                   \              tcgg"
FT   -               3603..3606
FT                   /note="tcgg 4bp [pBR327]
FT                   \   tcgg
FT                   XbaI = T^CTAGA"
FT   -               3607..5869
FT                   /note="pUC18 424..2686 2263bp"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-BamHI-XmaI-SmaI-KpnI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-BamHI-XmaI-SmaI-KpnI-SacI-
FT                   EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5869 BP; 1676 A; 1228 C; 1392 G; 1573 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc
     aacagtatct tccccttcac aaaattgcag caaaagctct aaaacaaaca caaaaaggcg
     ttgagctgtt tttttacttt cagtccatga cctacgaacc ttaacggagg cctggcgtga
     cagccggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
     tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     ctaggtcttg aaaggagtgc ctgggggaat attcctctga tgagaaaggc atatttaaaa
     aaatgcaagg agtttcatcc tgataaagga ggagatgaag aaaaaatgaa gaaaatgaat
     actctgtaca agaaaatgga agatggagta aaatatgctc atcaacctga ctttggaggc
     ttctgggatg caactgaggt atttgcttct tccttaaatc ctggtgttga tgcaatgtac
     tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg cttgctgtgc
     ttactgagga tgaagcatga aaatagaaaa ttatacagga aagatccact tgtgtgggtt
     gattgctact gcttcgattg ctttagaatg tggtttggac ttgatctttg tgaaggaacc
     ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatccatt
     ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta atccagaaga
     agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa
     atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca gttttgaaat
     gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg aaaagcatta
     tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt
     tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag aacaaatgtt
     aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt ctacaggctc
     tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt tgcccaaaat
     ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta aaaaaagata
     ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg ctttgcttga
     attatgtggg gggaaagctt taaatgttaa tttgcccttg gacaggctga actttgagct
     aggagtagct attgaccagt ttttagtagt ttttgaggat gtaaagggca ctggagggga
     gtccagagat ttgccttcag gtcagggaat taataacctg gacaatttaa gggattattt
     ggatggcagt gttaaggtaa acttagaaaa gaaacaccta aataaaagaa ctcaaatatt
     tccccctgga atagtcacca tgaatgagta cagtgtgcct aaaacactgc aggccagatt
     tgtaaaacaa atagatttta ggcccaaaga ttatttaaag cattgcctgg aacgcagtga
     gtttttgtta gaaaagagaa taattcaaag tggcattgct ttgcttctta tgttaatttg
     gtacagacct gtggctgagt ttgctcaaag tattcagagc agaattgtgg agtggaaaga
     gagattggac aaagagttta gtttgtcagt gtatcaaaaa atgaagttta atgtggctat
     gggaattgga gttttagatt ggctaagaaa cagtgatgat gatgatgaag acagccagga
     aaatgctgat aaaaatgaag atggtgggga gaagaacatg gaagactcag ggcatgaaac
     aggcattgat tcacagtccc aaggctcatt tcaggcccct cagtcctcac agtctgttca
     tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     tctcggctag aggatccccg ggtaccgagc tcgaattcgt aatcatggtc atagctgttt
     cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaaagctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//