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ID   PUCURA3    preliminary; circular DNA; SYN; 3858 BP.
XX
AC   IG9913;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pUC-URA3 - complete, fusion.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pG6 from yeast 2 micron ori & pBR322
RC   pG63 from pG6 & URA3 gene
RC   pG63.11 from pG63
RC   pHCG3 from pG63.11 & pHC79
RA   Gerbaud C., Elmerich C., Tandeau De Marsac N.T., Chocat P.,
RA   Charpin N., Guerineau M., Aubert J.P.;
RT   "Construction of new yeast vectors and cloning of the Nif (nitrogen
RT   fixation) gene cluster of Klebsiella pneumoniae in yeast";
RL   Curr. Genet. 3:173-180(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUC-URA3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 NarI 2686bp 238..238
FT                   2. YEp24 HindIII-HindIII 1170bp 2273..3442
FT                   -> pUC-URA3 3858bp"
FT   -               1..239
FT                   /note="pUC19 1..239 239bp
FT                   NarI = GG^CG CC = KasI = G^GCGCC
FT                   HindIII =  A^AGCTT"
FT   -               240..1409
FT                   /note="YEp24 2273..3442 1170bp
FT                   HindIII = A^AGCT T
FT                   NarI =        GG^CGCC = KasI = G^GCGCC"
FT   -               1410..3858
FT                   /note="pUC19 238..2686 2449bp"
FT   misc_feature    1..33
FT                   /note="MCS SacI-KpnI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3858 BP; 1049 A; 863 C; 958 G; 988 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgca
     gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga
     aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
     tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
     tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ttataatgac
     gaaaaaaaaa aaattggaaa gaaaaagctg ccattcgcca ttcaggctgc gcaactgttg
     ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
     gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtc
//