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ID   PUEX2      preliminary; circular DNA; SYN; 6728 BP.
XX
AC   X06291;
XX
DT   29-NOV-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pUEX2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6728
RC   pUEX2
RA   Stanley K.K.;
RT   ;
RL   Submitted (23-NOV-1987) on tape to the EMBL Data Library by:
RL   Stanley K.K., EMBL, Meyerhofstr. 1, Heidelberg, 6900, Germany.
XX
RN   [2]
RC   plasmid from M13mp9 & pOU110
RC   plasmid2 from plasmid & pLK915
RC   pUEX2 from plasmid2 & lacZ gene
RC   pUEX1 from pUEX2 & pEX1
RC   pUEX3 from pUEX2 & pEX3
RA   Bressan G.M., Stanley K.K.;
RT   "pUEX, a bacterial expression vector related to pEX with universal
RT   host specifity";
RL   Nucleic Acids Res. 15:10056-10056(1987).
XX
CC   NM (pUEX2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Amersham)
CC   HO (E.coli)(Gram-positive bacteria)
CC   CP ()
CC   FN (expression)
CC   SE (color blue/white)
CC   PA (pEX2)(pOU110)(M13mp9)(pLK915)
CC   BR (pUEX1)(pUEX3)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cI857 gene/pR/cro gene
FT                   -> pOU110
FT                   1. M13mp9 remove PstI-BglII 668bp 6617..7285, 6931bp
FT                   2. pOU110 PstI-BglII, cI857 gene/pR/cro gene
FT                   -> plasmid
FT                   1. pBR322
FT                   -> pLK915
FT                   1. plasmid remove 36bp, cro gene
FT                   mutagenesis
FT                   PstI-BglII, cI857 gene/pR/cro gene
FT                   2. pLK915 remove small PstI-BamHI
FT                   -> plasmid2
FT                   1. plasmid2 BamHI
FT                   2. E. coli BamHI-BamHI, lacZ gene
FT                   -> plasmid3
FT                   1. plasmid3 BamHI
FT                   fill in
FT                   -> pUEX2 6728bp"
FT   misc_feature    935..935
FT                   /note="lambda cI857 gene amino terminus"
FT   misc_feature    935..935
FT                   /note="lambda PR/PM transcription start site"
FT   misc_feature    946..962
FT                   /note="lambda region O(R)3"
FT   misc_feature    969..985
FT                   /note="lambda region O(R)2"
FT   misc_feature    993..1009
FT                   /note="lambda region O(R)1"
FT   misc_feature    1018..1018
FT                   /note="lambda PR transcription start site"
FT   misc_feature    1036..1036
FT                   /note="lambda cro gene amino terminus"
FT   CDS             1081..1194
FT                   /note="REP E. coli lacI repressor gene; fragment"
FT   CDS             1195..4140
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   fragment"
FT   misc_binding    4141..4176
FT                   /note="MCS"
FT   variation       4142..4145
FT                   /note="ADP AATT deleted in pUEX1"
FT   variation       4145..4146
FT                   /note="ADP AATT inserted in pUEX3"
FT   misc_feature    4179..4189
FT                   /note="TER stop codon region"
FT   terminator      4214..4316
FT                   /note="TER bacteriophage fd transcription
FT                   terminator 1"
FT   terminator      4323..4425
FT                   /note="TER bacteriophage fd transcription
FT                   terminator 2"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6728 BP; 1629 A; 1735 C; 1727 G; 1637 T; 0 other;
     ggtgatgatt atcagccagc agagaattaa ggaaaacaga caggtttatt gagcgcttat
     ctttcccttt atttttgctg cggtaagtcg cataaaaacc attcttcata attcaatcca
     tttactatgt tatgttctga ggggagtgaa aattccccta attcgatgaa gattcttgct
     caattgttat cagctatgcg ccgaccagaa caccttgccg atcagccaaa cgtctcttca
     ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca tgggatcatt
     gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat cagtttcttg
     aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc aacagcctgc
     tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc tgttggtgcg
     gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt tttggttgtg
     cttacccatc tctccgcatc acctttggta aaggttctaa gcttaggtga gaacatccct
     gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg ctgcatacta
     accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc ttcaacgcta
     actttgagaa tttttgtaag caatgcggcg ttataagcat ttaatgcatt gatgccatta
     aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga ttcctgggat
     aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc gtcctcaagc
     tgctcttgtg ttaatggttt cttttttgtg ctcatacgtt aaatctatca ccgcaaggga
     taaatatcta acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgcatg
     tactaaggag gttgtatgga acaacgcata accctgaaag atggatcatc cggagcttgg
     ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgacttaat
     cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
     cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
     acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
     cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
     gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg
     agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
     catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
     atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
     caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
     ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
     gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
     cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
     tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc
     ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
     tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
     ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg
     gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac
     tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac
     tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag
     caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     gaattcccgg ggatccgtcg acctgcagcc aagcttgctg attgattgac cggatcgatc
     cggctctaga attaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc
     ttttggagcc tttttttttg gagattttca acgtgaaaaa attattattc gcaattcaag
     ctaattcacc tagaaagcaa gctgataaac cgatacaatt aaaggctcct tttggagcct
     ttttttttgg agattttcaa cgtgaaaaaa ttattattcg caattcaagc tctgcctcgc
     gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     caagaatt
//