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ID   PUH24      preliminary; circular DNA; PRO; 7835 BP.
XX
AC   S89470; X63064;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Synechococcus plasmid vector pUH24 - complete.
XX
KW   cloning vector.
XX
OS   Synechococcus
OC   Cyanobacteria.
XX
RN   [1]
RC   pUC12 from pUC1 & RSF1010
RC   pUC13, pUC14 from pUC12
RA   Van Der Plas J., Oosterhoff-Teertstra R., Borrias M., Weisbeek P.;
RT   "Identification of replication and stability functions in the
RT   complete nucleotide sequence of plasmid pUH24 from the
RT   cyanobacterium Synechococcus sp. PCC 7942";
RL   Mol. Microbiol. 6:653-664(1992).
XX
RN   [2]
RC   pANS or pUH24 from A.nidulans R-2 or Synechococcus sp. PCC 7942
RC   pANL or pUH25 from A.nidulans R-2 or Synechococcus sp. PCC 7942
RC   pCH1, pCH2, pCH3, pCH4, pCH5 from pUH24 & pRI46, Tn901 amp gene
RC   pUC1 from pCH1
RA   Van Den Hondel C.A., Verbeek S., Van Den Ende A., Weisbeek P.J.,
RA   Borrias W.E., Van Arkel G.A.;
RT   "Introduction of transposon Tn901 into a plasmid of Anacystis
RT   nidulans: preparation for cloning in cyanobacteria";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:1570-1574(1980).
XX
RN   [3]
RC   pUC1 from pCH1
RC   pUC104, pUC105 from pACYC184 & pUC1
RA   Kuhlemeier C.J., Borrias W.E., Van Den Hondel C.A., Van Arkel G.A.;
RT   "Vectors for cloning in cyanobacteria: construction and
RT   characterization of two recombinant plasmids capable of
RT   transformation to Escherichia coli K-12 and Anacystis nidulans R-2";
RL   Mol. Gen. Genet. 184:249-254(1981).
XX
RN   [4]
RC   pRI477S from Tn901, strep gene
RC   pRI46 from pRI477S & Tn901, strep gene
RA   Van Embden J.D.;
RT   "Translocation of an ampicillin resistance determinant within an
RT   R-factor aggregate in Salmonella panama";
RL   Antonie Van Leeuwenhoek 44:203-218(1978).
XX
RN   [5]
RC   pJN60 from ColDF13 & Tn901
RA   Van Embden J.D., Veltkamp E., Stuitje T., Andreoli P.M., Nijkamp H.J.;
RT   "Integration of a transposable DNA sequence which mediates
RT   ampicillin resistance into Clo DF13 plasmid DNA: determination of
RT   the site and orientation of TnA insertions";
RL   Plasmid 1:204-217(1978).
XX
CC   NM (pUH24)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Synechococcus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Synechococcus)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. A.nidulans R-2 or Synechococcus sp. PCC 7942
FT                   -> pUH24 7835bp"
FT   rep_origin      0..0
FT                   /note="ORI Synechococcus"
FT   CDS             complement(join(7507..7835,1..163))
FT                   /note="GEN Synechococcus ORF H; #1 amino acid V to M;
FT                   NCBI gibbsq 89472 protein;
FT                   this sequence comes from Fig. 1.
FT                   [1] protein sequence is in conflict with the
FT                   conceptual translation."
FT   CDS             complement(574..1137)
FT                   /note="GEN Synechococcus ORF A; NCBI gibbsq 89486
FT                   protein; this sequence comes from Fig. 1."
FT   CDS             1485..1928
FT                   /note="GEN Synechococcus pmaA gene; for the pmaA
FT                   protein sequence (NCBI gibbsq 89489);
FT                   this sequence comes from Fig. 1."
FT   CDS             1938..2483
FT                   /note="GEN Synechococcus pmaB gene; ORF C;
FT                   #1 amino acid V to M.
FT                   for the protein sequence (NCBI gibbsq 89492);
FT                   this sequence comes from Fig. 1.
FT                   [1] protein sequence is in conflict with the
FT                   conceptual translation."
FT   CDS             complement(2644..3159)
FT                   /note="GEN Synechococcus ORF D; NCBI gibbsq 89494
FT                   protein; this sequence comes from Fig. 1."
FT   CDS             complement(3567..4040)
FT                   /note="GEN Synechococcus repB gene;
FT                   #1 amino acid V to M.
FT                   for the protein sequence (NCBI gibbsq 89495);
FT                   this sequence comes from Fig. 1.
FT                   [1] protein sequence is in conflict with the
FT                   conceptual translation."
FT   CDS             complement(3995..6625)
FT                   /note="GEN Synechococcus repA gene;
FT                   for the protein sequence (NCBI gibbsq 89496);
FT                   This sequence comes from Fig. 1."
FT   CDS             complement(6800..7402)
FT                   /note="GEN Synechococcus ORF G; #1 amino acid V to M;
FT                   NCBI gibbsq 89498 protein;
FT                   this sequence comes from Fig. 1.
FT                   [1] protein sequence is in conflict with the
FT                   conceptual translation"
XX
SQ   Sequence 7835 BP; 1565 A; 2321 C; 2336 G; 1613 T; 0 other;
     ggatccgcga gcgtgcttgc tgacgagttg caccgctgcg gatcagctca tccgcgaggg
     actgatcgag gcgcgcccgc gagacggcct tcacaatgtc agccgcccag cgctgctcgg
     ctgcagagcg ggtctgcgcg ttagggtctg caggaatcgc cacggcgctc acctccaaaa
     gccgccagcg gttcacaaca atcgagtcgc ggagatcgtc gtagtcagtc gagacaaagc
     gaacgctcca agagatcgca ccggaactga tcagctccca caggcgatcg cgatcgggat
     gttcgagcaa ggtgatcgtc gctagcagtc cagtttcccc gttctccagt cgctcagctc
     tgagcgccgt ggcagtacca acaacggctg aggttgaggc gtcgtgatcg gtcaaaacgg
     gcagcggatc aggtcgctcg atattggaga ggtcatcggc gatgactagc tgatgaccgt
     caatcagtgc cgcgctactg cgatcaccgc tgtcaggctg taagtcgcag ggtttaagga
     tgtgggcaat gctcgggtga gagccatttg gtcttacctg tagtgagggg cggaagtaga
     aggcttaggc ggtacgatcg ccttttcgag ttcgtttcgg gtttcaagac tgcggtgggt
     ttcccgcacg atcagcccgt gcagctcagt gaggagctgc agcatcagca gcccccctag
     gcggatctct aggccgtcca agcggagcca tgcgccacct tgtccactgg tctgaacctc
     aagaccaatc gagccgatcg aacttcgcac cggctcatcg ctgcggtcgg tgcctgccag
     tcgtcgggac gctgcaaccg atgcgccgat cacttccgcg atcgctgcca atctggggag
     gtctgcaggg acagcacgct cactagcaac cgtcaccacg ccaaactcac cgcgctcgca
     gtcgattaca aggaacgccg tttggccttg agagtcgcga ccggcgatcg ctagtgaacg
     tgtctggacg atcgcgccgg tgtcgatgct ggccattgcc cagcccatca tttctgggtc
     atcgctggtc agtcgcagcc ccccggcact acttggaaaa ccttcgatat ttggcatttt
     tagcctcgca atttgctgca agactagctg aaacagacaa gcgaaaactt aggcccagac
     tggcctagag ctgcttcaga attaccgctt gttcccgcta attcacgctt gttcctaaca
     gttcactgaa ctataggtaa aactcaatga acaaacactg aatgataaga aattcaaata
     gatcgaggtg gggataattg cctctctttg caggtgattg acgctgattg cagacggctg
     ggaggtggtt tttagcgagt aaatcgacgc tgatcagact tggggtaaga gtccacagcc
     gacgcagcaa aactaggccg gcccttaact aaaccccaag aaaaatgatt gaacctcacc
     acttctacga agcagcacta gccgccttct cccaaatcga ttggcgggct gcagtgaaca
     tctactctca gttgagccgc cttagcaggc aatcgtcagc gagtacgatg agggcacccg
     ctctgccctc gaccgtgacc gacaacacct cagatctcat ccttcagcga ctagacacga
     tcgatgcgcg gctaggccga atcgaggatc gtcttgatcg cgtcgaatcg gatctgtctg
     agctgaagac cgacgtcaaa gaggtgcgat cgtcactagc tgaattgact ggggacgttc
     gcaagattga aggcgtcctg caaggtgcag ggctgccaaa catcaacacg aggatcggca
     ttgccacggt gagcagcgcc gcaatggtgg cattctttct gatgctggcc gatcgctttc
     tggggtagct gttaacagtg ccaagccctt tagtccgagt gcctgacgca ctgcgcgatc
     aggtgttaac actgtgccgc cttcaccgtg aagggctgcc ggtaaaagcc gctctggaac
     aggcgatcgc tggcctgcaa gctgtagaca gccctgttaa cagcagccca catcagacag
     ccttggatga cctcgccaga cgggtcgaag ccttggagca gttaacagcc ctgttaacac
     cttcgggaga gcctgttaac acttctgcta acaggtctgt taacagcaag gcactctctg
     taaacagccc tgttaaccga aagtccgatt ctgttaacag ctctggcgac tggctaacgg
     ttgaggaggc tcatcaactt gcggctgagc ggggctgccc tgcgagtctg gccaccttca
     gacgttggag cagaggaaat gcgaagtatc cccagggcga tgtggcctcg ctgaagcaat
     gggggttcga tcgggatatg acccgattgg ccagcggcag ctcaaagaat ccggcgcggt
     tcctacgggc gatcgcagac tgagacaggg ctgtcttagt cgtgctgtct cacgcggcca
     agtttgatga atgccggatc taagccctga gcgctccgct cgttgtctca ggcagggtac
     ttagtccaat ggctgagggg gagcatctaa aggctaggta ggacagggct gatgacttgc
     tccctaggca gacacgccag acagcggctc caaagcctgc gatacgtctc gctgcagcaa
     cgcggcgatt gcctcccgct tctccggtgg cagttcccca gtgacggcct caatcgaccg
     cacacaagca gccggaaccg tcgcaaagcg atcgatcacc cgcttgctct cggctgccca
     agtgctcgcg atatcctcag catcaactag cgatcgctcg cgttcttcaa cgtcgagccg
     gagcagctcg gcgcggtagt gcgctttcag acgccgcgcc tctagttgct cggctgatgg
     aggctcacga cgcttgccac gcccgccacc atcgttcctg atccggctcg gatcggtgtt
     tgctgcccac tcgcgagccg cagcaaccgg atcgacgtgc caccgactgc cgaccttcca
     gactgaagtg atccgaccgg ctgcgatcgc ctgatgtatc gcctgaggcg caacgccatt
     agcgcgagca aactcgggca gggactggct ttcagccatg gctagagact ccttagatca
     agaacgacaa gcataaaaat ttcctatcag tggacaggtt tcgcgacgtt cggacgccgt
     acctggtgcc tatagggaag aacctaagca tttctactta gccatccgtc gcgacgggta
     cgaggtgcac ggccaagctg gagcggcctg tgggtgctgg ctggcagcca gagcagccgg
     caaggtggcc gagctgggca ctgacagcct cagtgagcgg ctctcggaag cgctcgagca
     ttggccacat gtgcttgggt gctgcgatgg ccagcgttcc actatcgaag ctgtaggcgc
     ggcacggtta gccgcaaacc atcgcaagcc ttcgttctcg atcgcggcga tcgcagttgt
     ccaaagctga tcgagttcgg cttgtgtcat gctgcggtct ccgtagatgt agtgctgacc
     tgacctgacg cggtgtcacg aaaatgcgca gtgattggtt ttgatcttct attagggatt
     tcgtgacagc tccccagtcc ctgactagat cgggcgtttt caagccaatg agcttcgatc
     gcctcaagcg gcagttcagc aatcgcctca ttcggcacga cttggtagat ccaagtggac
     ttgcccgcga tgcgcttctg ccgtgccttg agcttgaagc cgatcatccg cagcagcgcc
     cgcacgatgg tgctggctct ggtcttgtcg gtgatctgaa tcttgaggat ctgtgcggca
     ctagcccggt ggcttgtggc ggtcgtgacg atctcttgaa tcagcggatc atcaacgctg
     aaccagtcgc ggcgttgcag caactcttcc accttcagcc catgcaggta ccgcacttgg
     ggcgcgatac agctgcccac aagatcgggt ccgaacatcg ggcgatcgcg ccgtttgtcg
     agcttggcgg catcgcgagc ggcacggcat ccttgccaag gctgagccag aacagcagtc
     ttagcggggc gtatagaccg tcgtcatcag cggctagtag ttcacgggtg cagtctcccc
     agcgttgctg gcagcggtgc cgagcaaggg cagccaactg ttccgcgtta gcgaagtccg
     cagacgcgat cgcctcagct tccttcgggg tcagatcagg tgaactcagg atcgcctcgg
     ctgaggcatc agccagcgct tcagcagtct cccgttgcag ttcgtcaacg acctcttgaa
     gaaagggatc gatcgcgccg tcccagtctt gccaagtgaa cccgttgagc cgtagaagct
     gctccaccgt ggcggcatag gtcaacccgt cacggttgcg acgggcggcc aattctgccc
     atgcagtcaa gtaggcttgc gatgcgaccg gctcgccgat cttgatcgcc gcctgtgaca
     gctgagccaa gatcacttgt tggtgccgct tcaggccgtc ggcaacctcg gttgggtcga
     tgctaccgtt gccagctcgc atcaaattgg acgtgtcggg gcaggtgatg aatcggggga
     cgttgctccg cacccgtcct gcaatctgcg cgatcgcacc tggggcgatg tgcccagctc
     cggcgaagat cagcacggca tcaaatgggc gatcgcctac cccttgggtg atcgacaagc
     cagcatcgat cacgctcgtg aagacaaggt gggggcaaca gttgatgaac atctgcgggt
     tgagcttcag cgtttccaaa gccccccgct tattggcttc gctgtcgatg accggaaccg
     gctgaccgca ggcttcggag agctgttggg cgatcgtcgc tgcggaatac agggacggcc
     gcgactggct cagcttctgg cggtcgcaga agactgctaa gcgcttgccc gcctgaacca
     tttcgaccgc catcgccatg agttcgcgat cgctgccaac ctgatagacc ggagcaccgc
     tgaagggttg gacggtactg ctgaccagat atgcggttga tcctgtcagt gcttccaaca
     gggcgatcga cacatccgac agcatcgcgt cagcggcgat aacctgccgc ccccctgcga
     gtagtccagc aatttcagcc atgacttcgg ggcgatgttt agcgatctcg gtcttgccgc
     tcagcaggtg ctcagcccac agttccacct catcgatgac gacgatcgcc cgttgccatc
     cgtgggcgct gaagcgcagc ttgctggcgg ggtgcatgga gtctaggcaa ccgccaaggc
     cgatcatccg aaggtcggaa ccgggaacgc attcttcagc ccacggcacc cctaagcgct
     tgcccaaggc cgcgcccaag gtacggcgat gcgtgatcaa cacgacagga atgccggtac
     caatgagcgg ctggagatgc cccgcgatcg cctctgtctt gcccgtcccc atcgcagcat
     tcaggcagac aaggcgatgc tgttccggct gcgggatgta gtcgccgttg atgtacccgc
     cggtgtgtaa gcggttaggg ctacgggtca gccgctgcca ctcgttcgcg acctcgcgct
     gatattcccg ctgctcttgt cgagtcagct ttggcgggcg gtagccgcct tctttggcca
     gcttggccag tgtggcgatc gtgatgccgc tgcccttgaa gctggcccac ttccgctcta
     gcttgcggtt gccgtcgaac ttggacgatt gcgctgacca tgcaatccag tcatctaaca
     gggcatcgct gactgactgc agggccatcc cgacagccac ccagctctcg tagtcgtctg
     cgtagctggc aggtacatgg gccagcatgg tgcgggcgat cgcgacctca tcatcagaag
     ctggagcact gacgatcggc ttataggatt tcggcgcggt ctgccggtct tgccgctcca
     ttgcatccag caattcgggc ggggcctctg caacctcgat ctcccaaggt gcgcagcctt
     ccacccagcg atatgcgccg gtctcagggt gttgaccaac gatgactgat tgcagcttgt
     tccaacgcag ctctaacaga tcaccgtctg ggctgtgaag cttctgcgtg gcgatcgcag
     cccatcgctc ttggggtacc tgatagagcc gttggtggcg tcctggcttg ccagatgtat
     atgcaactgt gggcggtaat tctgtcagcg tcagcccttc tggcagtgcc tcatgagccg
     ttgggccatc gaagtcaact gcgattaacc caccgcttgc ggggccagta agcagaccga
     ttccggtaac gcgccgatcg ctgcggatac gttcagcaac agcagcatgg tcaagcgggt
     tattttgcca gccagggtca aatggtgcct tgcgactgtt aacgacgcag aagcgccagt
     ttgggggcag ccggtcaagt tgatcaatta attcgcagcg ggtcgggatc tgcgaccttt
     gcagatgatt ggcgataaat gccatacttc tgttgagtta tttttcctga cctcccctgg
     gcaaagtttg ccgacctggc ctgggggggg ttttgctttg attgccgata actatagctg
     gaatgaatac ggcaggtggc aaggctgcct cgaattgatc ggcaagccag agcgcgatcg
     cccctggctg tgatgagact tagacgccgg acgctgtcga gatccgaacg atcggacggt
     gatcgactac gcagcagccg aagctgtgct cagccttggc gcggtaaaag cccgtctcga
     actgcacctc gctagttagc tgaggcgcgg gcgtcaggcg tcccagctcc agaggtaggt
     tgccgcgtgc aactaggtag tagtagctgc cgctcagccg tgcgtcgatc gcaacctcca
     gcgaccaagg gttgaactca tcgcggctgt tgacttgcag atctgccacc aactgacgga
     taggcagctc actctcaggc gacgcgagga tcagtccagg ctcttgatcg agcaggcggc
     cgccgatcgt cgcctgactt ctcaacaacg cgatcgccgc acccaagctc ggaagcgtga
     tgccagtctc atccgtcgtc tcgttcaggt gctccggtgc gaacaaggcc aagccatcgg
     gtgcgggtgc tccgttggct gaaccttcga ttagggcaac cagtgctgcc acttcagccg
     ataggcaggc atcgcccagc tcagcgaagc cttgctgcag gccagcgaga tcatcgttgc
     ttaaggactc cggcgttgcc accagcccta ggccgaagcg ctgcggcttg tacttcgcga
     gcgagtcttc gacataggag aaggtgatct cgccaccttg ccggatcggg cgagcctcac
     cgactaagcc gtggcgacgg tcgaagcctc gcgatagtcg ttcagcggtc ggatgttcgc
     aatccgcagg acgccacgag ccggagcact aaagcgttgc tgcaggatgc gatcgccggt
     agattccagt gctgccggaa gatccgaggt gctcatcgca cgggcgatca cttgtgccgg
     agtgcggcct tggataccag ccgctcgcag tgcccaagcc aagctttctt ggccatcaaa
     agctctggcg acggcgttag cgatgggcga ctgccgctca cgggtcacat ggatctgggt
     cacgcttgga gcctcccggc tggctagctc ctcaa
//