back Return to this vector's summary.
ID   PUI108     preliminary; circular DNA; SYN; 14800 BP.
XX
AC   IG0119; ATCC37366;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pUI108 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pUI106 from pUI series]
RC   pUI108, pUI109 from pMC1403 & RSF1010 or pUI106
RA   Nano F.E., Shepherd W.D., Watkins M.M., Kuhl S.A., Kaplan S.;
RT   "Broad-host-range plasmid vector for the in vitro construction of
RT   transcriptional/translational lac fusions";
RL   Gene 34:219-226(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A broad host range plasmid vector for in vitro construction of
CC   transcriptional/translational lac fusions.
CC   Medium is 1065 LB medium.
CC   NM (pUI108)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (R.sphaeroides)(E.coli LG90)(E.coli)(broad host range)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (RSF1010)(pMC1403)
CC   BR (pUI109)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMC1403 EcoRI-SalI 6239bp 3640..9820..59,
FT                   \ lacZ gene
FT                   2. RSF1010 PvuII-PvuII 8557bp 1951..8684..1824
FT                   -> pUI108 14796bp [EcoRI, SmaI, SalI]"
FT   -               1..8557
FT                   /note="RSF1010 1951..8684..1823 8557bp
FT                   PvuII = CAG^CTG
FT                   EcoRI =   G^AATTC"
FT   -               8558..14800
FT                   /note="pMC1403 3640..9820..62 6243bp
FT                   SalI = G^TCGA C
FT                   PvuII =   CAG^CTG"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas R1162"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   CDS             6855..9904
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')
FT                   alpha peptide"
FT   CDS             9956..11209
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
XX
SQ   Sequence 14800 BP; 3179 A; 4243 C; 4288 G; 3090 T; 0 other;
     ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc
     tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc
     tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag
     agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc
     gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc
     tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg
     ccagccagtg cccgtcactc ccgtactaac tgtcacgaac ccctgcaata actgtcacgc
     ccccctgcaa taactgtcac gaacccctgc aataactgtc acgcccccaa acctgcaaac
     ccagcagggg cgggggctgg cggggtgttg gaaaaatcca tccatgatta tctaagaata
     atccactagg cgcggttatc agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc
     cggccagagg ccggatagct ggtctattcg ctgcgctagg ctacacaccg ccccaccgct
     gcgcggcagg gggaaaggcg ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat
     acgctggagc gcttttagcc gctttagcgg cctttccccc tacccgaagg gtgggggcgc
     gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag cccggctcat ccttctggcg
     tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc
     catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac
     cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg
     ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc
     cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat
     catggaggca cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg
     tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca
     ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg
     gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag
     cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg
     ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc
     aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag
     cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat
     acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag
     cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag
     accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag
     cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac
     gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac
     ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg
     gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca
     atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga
     gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat
     gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca
     gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag
     cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac
     cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct
     cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg
     aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
     ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
     gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
     tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
     tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
     ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
     aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
     ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
     gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
     caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
     gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
     ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
     cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc
     aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg
     gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc
     ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc
     accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg
     cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg
     atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc
     aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc
     gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc
     gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg
     ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc
     tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac
     gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc
     gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg
     cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca
     cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg
     cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga
     gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc
     catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca
     gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgaatgcc atgtggtcaa
     aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct
     caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg
     ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg
     cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt
     gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca
     gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg
     cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt
     gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg
     caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca
     agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct tctgctcctg
     cagctaatgg atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat
     ccgtatgata ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca
     tcattttcgg catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg
     cgccagacgc agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg
     acctggtccg gcatccagca atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc
     gcgtatgcgc cggtgctgga cgcgctcagg cagatggcat tcccgtctcg ctcgacagtt
     atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc
     gcggttttcc agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg
     ttatgcattc ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca
     tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca
     aacgcaaccg ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa
     cctcgctctc ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc
     ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggtgt
     cggggccgcg acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg
     cacacacgag ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac
     cgcaagaatt cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa
     actgaaggaa cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg
     gttgcctgtc agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc
     cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct
     catttggctc aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca
     ggagggtgca tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg
     agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct
     atcggttgat caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga
     tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc
     gctgcacgat cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg
     caccgatatg gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa
     aactcttcaa tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc
     cgatttggca ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga
     gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct
     tgccttctat ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt
     cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt
     tggaaagttt cattgccaga cgggactcct gcaatcgtca agggattgaa acctatagaa
     gacattgctg atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca
     gtccggttgc tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg
     ctctctcaca tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta
     atggcgaagc tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg
     gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact
     gactacgtcc acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt
     gggctacatg gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg
     atagatcccg tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat
     ccggctgaca gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca
     ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg
     tgcctttccg cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg
     gccgcgatca agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc
     ctgggaacac atcaatctca ccggagaata tcgctggcca aagccttagc gtaggattcc
     gccccttccc gcaaacgacc ccaaacagga aacgcagaat tcccggggat cccgtcgttt
     tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc
     cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat
     ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt
     ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg
     acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct
     ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc
     gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca
     ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac
     tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg
     cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg
     gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
     ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
     cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc
     tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
     ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
     ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc
     gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta
     aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg
     tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg
     attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc
     gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
     tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
     tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg
     ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
     gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt
     ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
     tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
     ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
     tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg tttgcgccgt
     tctttatttt tatcttcggg ccactgttac aatacaacat tttagtagga tcgattgttg
     gtggtattta tctaggcttt tgttttaacg ccggtgcgcc agcagtagag gcatttattg
     agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg
     gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat cagtttgttt
     tctggctggg ctctggctgt gcactcatcc tcgccgtttt actctttttc gccaaaacgg
     atgcgccctc ttctgccacg gttgccaatg cggtaggtgc caaccattcg gcatttagcc
     ttaagctggc actggaactg ttcagacagc caaaactgtg gtttttgtca ctgtatgtta
     ttggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc tttacttcgt
     tctttgctac cggtgaacag ggtacgcggg tatttggcta cgtaacgaca atgggcgaat
     tacttaacgc ctcgattatg ttctttgcgc cactgatcat taatcgcatc ggtgggaaaa
     acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca tcgttcgcca
     cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta ccgttcctgc
     tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca gcgacgattt
     atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct gtactggcgg
     gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt ctggtggcgc
     tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggcccgctt tccctgctgc
     gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg atgcggcgcg acgcttatcc
     gaccaacata tcataacgga gtgatcgcat tgaacatgcc aatgaccgaa agaataagag
     caggcaagct atttaccgat atgtgcgaag gcttaccgga aaaaagactt cgtgggaaaa
     cgttaatgta tgagtttaat cactcgcatc catcagaagt tgaaaaaaga gaaagcctga
     ttaaagaaat gtttgccacg gtaggggaaa acgcctgggt agaaccgcct gtctatttct
     cttacggttc caacatccat ataggccgca atttttatgc aaatttcaat ttaaccattg
     tcgatgacta cacggtaaca atcggtgata acgtactgat tgcacccaac gttactcttt
     ccgttacggg acaccctgta caccatgaat tgagaaaaaa cggcgagatg tactcttttc
     cgataacgat tggcaataac gtctggatcg gaagtcatgt ggttattaat ccaggcgtca
     ccatcgggga taattctgtt attggcgcgg gtagtatcgt cacaaaagac attccaccaa
     acgtcgtggc ggctggcgtt ccttgtcggg ttattcgcga aataaacgac cgggataagc
     actattattt caaagattat aaagttgaat cgtcagttta aattataaaa attgcctgat
     acgctgcgct tatcaggcct acaagttcag cgatctacat tagccgcatc cggcatgaac
     aaagcgcagg aacaagcgtc gcatcatgcc tctttgaccc acagctgcgg aaaacgtact
     ggtgcaaaac gcagggttat gatcatcagc ccaacgacgc acagcgcatg aaatgcccag
     tccatcaggt aattgccgct gatactacgc agcacgccag aaaaccacgg ggcaagcccg
     gcgatgataa aaccgattcc ctgcataaac gccaccagct tgccagcaat agccggttgc
     acagagtgat cgagcgccag cagcaaacag agcggaaacg cgccgcccag acctaaccca
     cacaccatcg cccacaatac cggcaattgc atcggcagcc agataaagcc gcagaacccc
     accagttgta acaccagcgc cagcattaac agtttgcgcc gatcctgatg gcgagccata
     gcaggcatca gcaaagctcc tgcggcttgc ccaagcgtca tcaatgccag taaggaaccg
     ctgtactgcg cgctggcacc aatctcaata tagaaagcgg gtaaccaggc aatcaggctg
     gcgtaaccgc cgttaatcag accgaagtaa acacccagcg tccacgcgcg gggagtgaat
     accacgcgaa ccggagtggt tgttgtcttg tgggaagagg cgacctcgcg ggcgctttgc
     caccaccagg caaagagcgc aacaacggca ggcagcgcca ccaggcgagt gtttgatacc
     aggtttcgct atgttgaact aaccagggcg ttatggcggc accaagccca ccgccgccca
     tcagagccgc ggaccacagc cccatcacca gtggcgtgcg ctgctgaaac cgccgtttaa
     tcaccgaagc atcaccgcct gaatgatgcc gatccccacc ccaccaagca gtgcgctgct
     aagcagcagc gcactttgcg ggtaaagctc acgcatcaat gcaccgacgg caatcagcaa
     cagactgatg gcgacactgc gacgttcgct gacatgctga tgaagccagc ttccggccag
     cgccagcccg cccatggtaa ccaccggcag agcggtaacc gcacttatga ctgtcttctt
     tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
//