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ID   PUR222     preliminary; circular DNA; SYN; 2700 BP.
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AC   L09145; VB0139; IG0122; VB0139; ATCC37116;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUR222 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC4 from pUR2 & M13mp7
RC   pUK201 from pUR2 & lac operon
RC   [p1Cm from cat gene]
RC   pUR168 from pBR322 & p1Cm, cat gene
RC   pUR2-Pst from M13mp2-PstI & pUR1
RC   CmrM13mp7 from pUR168 & M13mp7
RC   pUK201 from CmrM13mp7
RC   pUK213 from pUK201
RC   pUR213 from pUR168 & M13mp7
RC   pUK217 from pUK213 & pUR2-Pst
RC   pUR219 from pUK217 & pUC4
RC   pUR222 from pUK217 & pUR219
RA   Ruther U., Koenen M., Otto K., Muller-Hill B.;
RT   "pUR222, a vector for cloning and rapid chemical sequencing of DNA";
RL   Nucleic Acids Res. 9:4087-4098(1981).
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RN   [2]
RP   1-2700
RC   pUR222
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Vector for cloning and rapid chemical sequencing of DNA. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   old Boehringer vector.
CC   NM (pUR222)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM83)(E.coli JM103)(E.coli JM105)(E.coli FM15)(E.coli K802)
CC   HO (E.coli lacZdelta M15 deletion)(E.coli F-Z-deltaM15recA)(E.coli)
CC   CP ()
CC   FN (sequencing Maxam-Gilbert)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR ()
CC   OF (pUK217)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR1 2223bp
FT                   2. M13mp2, MCS
FT                   -> pUR2 2300bp
FT                   1. pUR2 2300bp
FT                   2. E. coli, lac operon but no lacI
FT                   -> pUK201 [EcoRI-BamHI-EcoRI in 5' lacZ]
FT                   1. phage P1
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> phage P1Cm
FT                   1. pBR322 PstI 4361bp 3612..3612, amp gene
FT                   2. phage P1Cm PstI-PstI 800bp, cat gene
FT                   -> pUR168 5200bp
FT                   1. M13mp2 7196bp
FT                   fill in
FT                   -> M13mp2-PstI 7196bp
FT                   1. M13mp2-PstI HaeII-HaeII 391bp 6006..6397, lac
FT                   2. pUR1 HaeII 2223bp 216..216, may be 586..586
FT                   -> pUR2-Pst 2614bp
FT                   1. pUR168 PstI-PstI 800bp, cat gene
FT                   2. M13mp7 PstI 7238bp 6257..6257
FT                   -> CmrM13mp7 8000bp
FT                   1. CmrM13mp7 BamHI-BamHI 800bp, cat gene
FT                   2. pUK201 BamHI, lac operon
FT                   -> pUK213
FT                   1. pUK213 PstI, amp gene
FT                   2. pUR2-Pst PstI-PstI, lacPO/amp
FT                   -> pUK217
FT                   1. pUC4 HindII 2605bp 2159..2159
FT                   mutagenesis
FT                   remove AccI-AccI 139bp 343..482, 2466bp
FT                   Klenow
FT                   remove HaeII-HaeII 370bp 603..973, 2096bp
FT                   \ pUC4 HaeII = 170 603 973
FT                   -> pUR219 2096bp [no HindII, no AccI, no PstI]
FT                   1. pUK217 HaeII-HaeII 600bp, lacPO/lacZ
FT                   \ M13mp7 HaeII = 2715 3044 5564 5572 6006 6439
FT                   2. pUR219 HaeII 2096bp
FT                   -> pUR222 2700bp"
FT   CDS             join(2637..2700,1..614)
FT                   /note="ANT E. coli beta-lactamase gene (bla E-286)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    55..55
FT                   /note="SIT unique Asp700I"
FT   misc_binding    174..174
FT                   /note="SIT unique ScaI"
FT   misc_binding    432..432
FT                   /note="SIT unique AvlII"
FT   misc_binding    570..570
FT                   /note="SIT unique Cfr10I"
FT   misc_feature    1073..1464
FT                   /note="pBR322 HaeII fragment"
FT   misc_binding    1440..1440
FT                   /note="SIT unique DrdI"
FT   rep_origin      complement(>1440..<1542)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1542..1542
FT                   /note="SIT unique AflIII"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacZ gene"
FT   CDS             complement(>1542..<2087)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS PstI-SalI-AccI-HindIII-EcoRI-BamHI"
FT   misc_binding    1826..1859
FT                   /note="MCS unique EcoRI-BamHI-SalI-PstI"
FT   promoter        complement(>1542..<2087)
FT                   /note="PRO E. coli lac gene"
FT   misc_feature    join(2087..2700,1..1227)
FT                   /note="pBR322 EcoRI to PvuII fragment"
FT   misc_binding    2087..2087
FT                   /note="SIT unique NarI"
FT   misc_binding    2090..2090
FT                   /note="SIT unique BbeI"
FT   misc_binding    2139..2139
FT                   /note="SIT unique NdeI"
FT   misc_binding    2211..2211
FT                   /note="SIT unique BsaAI"
FT   misc_binding    2217..2217
FT                   /note="SIT unique AspI"
FT   misc_binding    2375..2375
FT                   /note="SIT unique DraII"
FT   misc_binding    2378..2378
FT                   /note="SIT unique PssI"
FT   misc_binding    2436..2436
FT                   /note="SIT unique AatII"
FT   misc_binding    2550..2550
FT                   /note="SIT unique SspI"
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SQ   Sequence 2700 BP; 656 A; 667 C; 699 G; 678 T; 0 other;
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgatgccg
     ggcaagagca actcggtcgc gcgatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatcccta
     acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     gaactctgta gcaccgccta catacctcgt cctgctaatc ctgttaccag tggctgctgc
     cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gccattcgcc
     attcaggcta cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     gctggcgaag gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     gtcacgacgt tgtaaaacga cggccagtga attccccgga tccgtcgacc tgcagcaatt
     cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaagtca
     cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgg
     attaatgaat cggccaacgc gccgggagag gcggtttgcg tattgggcgc ctgatgcggt
     attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     tctgctctga tgccgcatag ttaagccagt aatacactcc gctatcgcta cgtgactggg
     tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgatgg gcttgtctgc
     tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
//