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ID   PUR278     preliminary; circular DNA; SYN; 5351 BP.
XX
AC   L09146; A10302; VB0133;
XX
DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUR278 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5351
RC   pUR278
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pUR259 from pUR250
RC   pUR266/1, pUR266/2, pUR266/3 from pUR259
RC   pUR270 from pUK217
RC   pUR274 from pUR270 & pUR266/1 & pBR322
RC   pUR276 from pUR270 & pUR266/2 & pBR322
RC   pUR286 from pUR270 & pUR266/3 & pBR322
RC   pUR278 from pUR274 & pUR250
RC   pUR288 from pUR276 & pUR250
RC   pUR289 from pUR286 & pUR250
RC   pUR280 from pUR278
RC   pUR290 from pUR274 & pUR280
RC   pUR291 from pUR276 & pUR280
RC   pUR292 from pUR286 & pUR280
RA   Ruther U., Muller-Hill B.;
RT   "Easy identification of cDNA clones";
RL   EMBO J. 2:1791-1794(1983).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The ClaI and EcoRI sites in the polylinker region are not unique
CC   NM (pUR278)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUR274, from pUR270 and pUR266/1 and pBR322, and pUR250)
CC   BR (pUR288)(pUR289)
CC   OF (pUR290)(pUR291)(pUR292)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR250 remove EcoRI-HincII 17bp 327..344,
FT                   \ 2571bp
FT                   \ pUC4 EcoRI = 327 369
FT                   \ pUC4 HincII = 344 356 2159
FT                   2. E. coli EcoRI-HaeIII, lacZ gene 3'/IVS
FT                   -> pUR259
FT                   1. pUR259 HindIII
FT                   BAL31 nuclease
FT                   EcoRI-EcoRI
FT                   2. pUC9 remove SmaI-EcoRI 3bp 261..264, MCS/2662bp
FT                   -> pUR266/1
FT                   1. pUR1 2223bp
FT                   2. M13mp2, MCS
FT                   -> pUR2 2300bp
FT                   1. pUR2 2300bp
FT                   2. E. coli, lac operon but no lacI
FT                   -> pUK201 [EcoRI-BamHI-EcoRI in 5' lacZ]
FT                   1. phage P1
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> phage P1Cm
FT                   1. pBR322 PstI 4361bp 3612..3612, amp gene
FT                   2. phage P1Cm PstI-PstI 800bp, cat gene
FT                   -> pUR168 5200bp
FT                   1. M13mp2 7196bp
FT                   fill in
FT                   -> M13mp2-PstI 7196bp
FT                   1. M13mp2-PstI HaeII-HaeII 391bp 6006..6397, lac
FT                   2. pUR1 HaeII 2223bp 216..216, may be 586..586
FT                   -> pUR2-Pst 2614bp
FT                   1. pUR168 PstI-PstI 800bp, cat gene
FT                   2. M13mp7 PstI 7238bp 6257..6257
FT                   -> CmrM13mp7 8000bp
FT                   1. CmrM13mp7 BamHI-BamHI 800bp, cat gene
FT                   2. pUK201 BamHI, lac operon
FT                   -> pUK213
FT                   1. pUK213 PstI, amp gene
FT                   2. pUR2-Pst PstI-PstI, lacPO/amp
FT                   -> pUK217
FT                   1. pUK217 remove EcoRI-EcoRI, lacZ gene 3' end
FT                   \ M13mp7 EcoRI = 6232 6274
FT                   -> pUR270
FT                   1. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT                   2. pUR266/1 HindIII-EcoRI, PSB
FT                   3. pUR270 EcoRI
FT                   -> pUR274
FT                   1. pUR274 remove BamHI-HindIII, MCS
FT                   2. pUR250 BamHI-HindIII, MCS
FT                   \ pUC4 BamHI = 336 360
FT                   \ pUC4 HindIII = none
FT                   -> pUR278 5351bp"
FT   misc_feature    1..3287
FT                   /note="from EcoLac"
FT   misc_binding    3288..3307
FT                   /note="MCS BamHI-SalI-XbaI-HindIII-(ClaI)-(EcoRI)"
FT   misc_feature    complement(3308..3341)
FT                   /note="from pBR322"
FT   misc_feature    complement(3342..5351)
FT                   /note="from pBR322"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3619..4407
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5351 BP; 1259 A; 1384 C; 1463 G; 1245 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
     agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaaggg gatccgtcga
     ctctagaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
     ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     gaagcggaag a
//