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ID   PUR289     preliminary; circular DNA; SYN; 5347 BP.
XX
AC   L09148; A10304; VB0135;
XX
DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUR289 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5347
RC   pUR289
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pUR259 from pUR250
RC   pUR266/1, pUR266/2, pUR266/3 from pUR259
RC   pUR270 from pUK217
RC   pUR274 from pUR270 & pUR266/1 & pBR322
RC   pUR276 from pUR270 & pUR266/2 & pBR322
RC   pUR286 from pUR270 & pUR266/3 & pBR322
RC   pUR278 from pUR274 & pUR250
RC   pUR288 from pUR276 & pUR250
RC   pUR289 from pUR286 & pUR250
RC   pUR280 from pUR278
RC   pUR290 from pUR274 & pUR280
RC   pUR291 from pUR276 & pUR280
RC   pUR292 from pUR286 & pUR280
RA   Ruther U., Muller-Hill B.;
RT   "Easy identification of cDNA clones";
RL   EMBO J. 2:1791-1794(1983).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The ClaI and EcoRI sites in the polylinker region are not unique
CC   NM (pUR289)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUR286, from pUR270 and pUR266/3 and pBR322, and pUR250)
CC   BR (pUR278)(pUR288)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR250 remove EcoRI-HincII 17bp 327..344,
FT                   \ 2571bp
FT                   \ pUC4 EcoRI = 327 369
FT                   \ pUC4 HincII = 344 356 2159
FT                   2. E. coli EcoRI-HaeIII, lacZ gene 3'/IVS
FT                   -> pUR259
FT                   1. pUR259 HindIII
FT                   BAL31 nuclease
FT                   EcoRI-EcoRI
FT                   2. pUC9 remove SmaI-EcoRI 3bp 261..264, MCS/2662bp
FT                   -> pUR266/3
FT                   1. pUR1 2223bp
FT                   2. M13mp2, MCS
FT                   -> pUR2 2300bp
FT                   1. pUR2 2300bp
FT                   2. E. coli, lac operon but no lacI
FT                   -> pUK201 [EcoRI-BamHI-EcoRI in 5' lacZ]
FT                   1. phage P1
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> phage P1Cm
FT                   1. pBR322 PstI 4361bp 3612..3612, amp gene
FT                   2. phage P1Cm PstI-PstI 800bp, cat gene
FT                   -> pUR168 5200bp
FT                   1. M13mp2 7196bp
FT                   fill in
FT                   -> M13mp2-PstI 7196bp
FT                   1. M13mp2-PstI HaeII-HaeII 391bp 6006..6397, lac
FT                   2. pUR1 HaeII 2223bp 216..216, may be 586..586
FT                   -> pUR2-Pst 2614bp
FT                   1. pUR168 PstI-PstI 800bp, cat gene
FT                   2. M13mp7 PstI 7238bp 6257..6257
FT                   -> CmrM13mp7 8000bp
FT                   1. CmrM13mp7 BamHI-BamHI 800bp, cat gene
FT                   2. pUK201 BamHI, lac operon
FT                   -> pUK213
FT                   1. pUK213 PstI, amp gene
FT                   2. pUR2-Pst PstI-PstI, lacPO/amp
FT                   -> pUK217
FT                   1. pUK217 remove EcoRI-EcoRI, lacZ gene 3' end
FT                   \ M13mp7 EcoRI = 6232 6274
FT                   -> pUR270
FT                   1. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT                   2. pUR266/3 HindIII-EcoRI, PSB
FT                   3. pUR270 EcoRI
FT                   -> pUR286
FT                   1. pUR286 remove BamHI-HindIII, MCS
FT                   2. pUR250 BamHI-HindIII, MCS
FT                   \ pUC4 BamHI = 336 360
FT                   \ pUC4 HindIII = none
FT                   -> pUR289 5347bp"
FT   misc_feature    1..3283
FT                   /note="from EcoLac"
FT   misc_binding    3284..3303
FT                   /note="MCS BamHI-SalI-XbaI-HindIII-(ClaI)-(EcoRI)"
FT   misc_feature    complement(3304..3337)
FT                   /note="from pBR322"
FT   misc_feature    complement(3338..5347)
FT                   /note="from pBR322"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3615..4403
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5347 BP; 1255 A; 1384 C; 1463 G; 1245 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
     agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaggggatc cgtcgactct
     agaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
     tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     cggaaga
//