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ID   PUR292     preliminary; circular DNA; SYN; 5349 BP.
XX
AC   L09151; A10307; VB0138;
XX
DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUR292 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5349
RC   pUR292
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RC   pUR259 from pUR250
RC   pUR266/1, pUR266/2, pUR266/3 from pUR259
RC   pUR270 from pUK217
RC   pUR274 from pUR270 & pUR266/1 & pBR322
RC   pUR276 from pUR270 & pUR266/2 & pBR322
RC   pUR286 from pUR270 & pUR266/3 & pBR322
RC   pUR278 from pUR274 & pUR250
RC   pUR288 from pUR276 & pUR250
RC   pUR289 from pUR286 & pUR250
RC   pUR280 from pUR278
RC   pUR290 from pUR274 & pUR280
RC   pUR291 from pUR276 & pUR280
RC   pUR292 from pUR286 & pUR280
RA   Ruther U., Muller-Hill B.;
RT   "Easy identification of cDNA clones";
RL   EMBO J. 2:1791-1794(1983).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The ClaI and EcoRI sites in the polylinker region are not unique
CC   NM (pUR292)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUR286, from pUR270 & pUR266/3 & pBR322, and pUR280 from pUR278)
CC   BR (pUR290)(pUR291)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR278 PstI 5351bp 4096..4096, amp gene
FT                   NG-mutagenesis
FT                   remove EcoRI-EcoRI 105bp 3235..3340, lacZ gene 3' end
FT                   -> pUR280 5246bp [unique PstI]
FT                   1. pUR278
FT                   -> pUR280
FT                   1. pUR278 small EcoRI-HindIII 74bp 3235..3309, MCS
FT                   \ may be 3309..3340
FT                   2. pUR280 remove small EcoRI-HindIII 74bp 3235..3309,
FT                   \ MCS/5172bp
FT                   -> pUR292 5349bp"
FT   misc_feature    1..3283
FT                   /note="from EcoLac"
FT   misc_binding    3284..3305
FT                   /note="MCS BamHI-SalI-XbaI-HindIII-(ClaI)-(EcoRI)"
FT   misc_feature    complement(3306..3339)
FT                   /note="from pBR322"
FT   misc_feature    complement(3340..5349)
FT                   /note="from pBR322"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3617..4405
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5349 BP; 1254 A; 1386 C; 1464 G; 1245 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
     agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaggggatc cgtcgacctg
     cagccaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
     cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     agcggaaga
//