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ID   PUT18      preliminary; circular DNA; SYN; 2681 BP.
XX
AC   D13250;
XX
DT   02-OCT-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUT18 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2681
RC   pUT18 from pBR322
RA   Omori K., Akatsuka H., Komatsubara S.;
RT   "Versatile Escherichia coli expression vectors for production of
RT   truncated proteins";
RL   Plasmid 31:297-299(1994).
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RN   [2]
RC   pUT18
RA   Omori K.;
RT   ;
RL   Submitted (17-SEP-1992) to GenBank by:
RL   Omori K., Research Laboratory of Applied Biochemistry,
RL   Tanabe Seiyaku Co. Ltd., 16-89 Kashima-3-chome, Yodogawa-ku,
RL   Osaka 532, JAPAN.
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CC   NM (pUT18)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18, ori/amp/lacZ
FT                   2. pBR322
FT                   -> pUT18 2681bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2681 BP; 662 A; 695 C; 648 G; 676 T; 0 other;
     aattctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacatta tacgagccga
     tgattaattg tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca
     aaaaacatta tccagaacgg gagtgcgcct tgagcgacac gaattatgca gtgatttacg
     acctgcacag ccataccaca gcttccgatg gctgcctgac gccagaagca ttggtgcacc
     gtgcagtcga taagcccggg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaaagagt ttgtagaaac
     gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc
     gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc
     ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg
     agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg
     tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc ttctgcgttc
     tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacaga agcttgcatg
     cctgcaggtc gactctagag gatccccggg taccgagctc g
//