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ID   PUT332     preliminary; circular DNA; SYN; 7484 BP.
XX
AC   IG9867;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pUT332 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUT332 from yeast 2 micron & URA3 gene
RA   Sabatier P.;
RT   ;
RL   Unpublished (1990).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pUT332)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (yeast 2 micron)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron HindIII 6318bp 2332..2332
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pUT332 7484bp"
FT   -               1..2331
FT                   /note="yeast 2 micron 1..2331 2331bp
FT                   HindIII = A^AGCTT"
FT   -               2332..3497
FT                   /note="YEp24 2273..3438 1166bp
FT                   HindIII = A^AGCTT"
FT   -               3498..7484
FT                   /note="yeast 2 micron 2332..6318 3987bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 7484 BP; 2258 A; 1470 C; 1449 G; 2307 T; 0 other;
     gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
     ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
     tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
     gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
     gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
     acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
     aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
     aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
     ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
     aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     ttcgctcctt gaacttgcat ctaaactcga cctctacatc aacaggcttc caatgctctt
     caaattttac tgtcaagtag acccatacgg ctgtaatatg ctgctcttca taatgtaagc
     ttatctttat cgaatcgtgt gaaaaactac taccgcgata aacctttacg gttccctgag
     attgaattag ttcctttagt atatgataca agacactttt gaactttgta cgacgaattt
     tgaggttcgc catcctctgg ctatttccaa ttatcctgtc ggctattatc tccgcctcag
     tttgatcttc cgcttcagac tgccattttt cacataatga atctatttca ccccacaatc
     cttcatccgc ctccgcatct tgttccgtta aactattgac ttcatgttgt acattgttta
     gttcacgaga agggtcctct tcaggcggta gctcctgatc tcctatatga cctttatcct
     gttctctttc cacaaactta gaaatgtatt catgaattat ggagcaccta ataacattct
     tcaaggcgga gaagtttggg ccagatgccc aatatgcttg acatgaaaac gtgagaatga
     atttagtatt attgtgatat tctgaggcaa ttttattata atctcgaaga taagagaaga
     atgcagtgac ctttgtattg acaaatggag attccatgta tctaaaaaat acgcctttag
     gccttctgat accctttccc ctgcggttta gcgtgccttt tacattaata tctaaaccct
     ctccgatggt ggcctttaac tgactaataa atgcaaccga tataaactgt gataattctg
     ggtgatttat gattcgatcg acaattgtat tgtacactag tgcaggatca ggccaatcca
     gttctttttc aattaccggt gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg
     ctaacgtttt gtatttctta taattgtcag gaactggaaa agtccccctt gtcgtctcga
     ttacacacct actttcatcg tacaccatag gttggaagtg ctgcataata cattgcttaa
     tacaagcaag cagtctctcg ccattcatat ttcagttatt ttccattaca gctgatgtca
     ttgtatatca gcgctgtaaa aatctatctg ttacagaagg ttttcgcggt ttttataaac
     aaaactttcg ttacgaaatc gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa
     agtagagcaa cgcgagttgc attttttaca ccataatgca tgattaactt cgagaaggga
     ttaaggctaa tttcactagt atgtttcaaa aacctcaatc tgtccattga atgccttata
     aaacagctat agattgcata gaagagttag ctactcaatg ctttttgtca aagcttttca
     attcaattca tcattttttt tttattcttt tttttgattt cggtttcttt gaaatttttt
     tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt
     atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact
     gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga
     acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa
     gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt
     tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt
     ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact
     cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg
     tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg
     tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt
     gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac
     tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat
     gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga
     caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc
     tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg
     tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta
     aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
     attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa
     aaaaattgga aagaaaaagc ttactgatga tgatgtgtct actttcaggc gggtctgtag
     taaggagaat gacattataa agctggcact tagaattcca cggactatag actatactag
     tatactccgt ctactgtacg atacacttcc gctcaggtcc ttgtccttta acgaggcctt
     accactcttt tgttactcta ttgatccagc tcagcaaagg cagtgtgatc taagattcta
     tcttcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact
     tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg
     aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg
     tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
     acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
     ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
     tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
     gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
     tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
     tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
     ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
     ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
     ctttgatatt ggatcatacc ctagaagtat tacgtgattt tctgcccctt accctcgttg
     ctactctcct ttttttcgtg ggaaccgctt tagggccctc agtgatggtg ttttgtaatt
     tatatgctcc tcttgcattt gtgtctctac ttcttgttcg cctggaggga acttcttcat
     ttgtattagc atggttcact tcagtccttc cttccaactc actctttttt tgctgtaaac
     gattctctgc cgccagttca ttgaaactat tgaatatatc ctttagagat tccgggatga
     ataaatcacc tattaaagca gcttgacgat ctggtggaac taaagtaagc aattgggtaa
     cgacgcttac gagcttcata acatcttctt ccgttggagc tggtgggact aataactgtg
     tacaatccat ttttctcatg agcatttcgg tagctctctt cttgtctttc tcgggcaatc
     ttcctattat tatagcaata gatttgtata gttgctttct attgtctaac agcttgttat
     tctgtagcat caaatctatg gcagcctgac ttgcttcttg tgaagagagc ataccatttc
     caatcgaatc aaacctttcc ttaaccatct tcgcagcagg caaaattacc tcagcactgg
     agtcagaaga tacgctggaa tcttctgcgc tagaatcaag accatacggc ctaccggttg
     tgagagattc catgggcctt atgacatatc ctggaaagag tagctcatca gacttacgtt
     tactctctat atcaatatct acatcaggag caatcatttc aataaacagc cgacatacat
     cccagacgct ataagctgta cgtgctttta ccgtcagatt cttggctgtt tcaatgtcgt
     ccattttggt tttcttttac cagtattgtt cgtttgataa tgtattcttg cttattacat
     tataaaatct gtgcagatca catgtcaaaa caacttttta tcacaagata gtaccgcaaa
     acgaacctgc gggccgtcta aaaattaagg aaaagcagca aaggtgcatt tttaaaatat
     gaaatgaaga taccgcagta ccaattattt tcgcagtaca aataatgcgc ggccggtgca
     tttttcgaaa gaacgcgaga caaacaggac aattaaagtt agtttttcga gttagcgtgt
     ttgaatactg caagatacaa gataaataga gtagttgaaa ctagatatca attgcacaca
     agatcggcgc taagcatgcc acaatttggt atattatgta aaacaccacc taaggtgctt
     gttcgtcagt ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc attatgtgct
     gctgaactaa cctatttatg ttggatgatt acacataacg gaacagcaat caagagagcc
     acattcatga gctataatac tatcataagc aattcgctga gtttcgatat tgtcaataaa
     tcactccagt ttaaatacaa gacgcaaaaa gcaacaattc tggaagcctc attaaagaaa
     ttgattcctg cttgggaatt tacaattatt ccttactatg gacaaaaaca tcaatctgat
     atcactgata ttgtaagtag tttgcaatta cagttcgaat catcggaaga agcagataag
     ggaaatagcc acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga aagcatctgg
     gagatcactg agaaaatact aaattcgttt gagtatactt cgagatttac aaaaacaaaa
     actttatacc aattcctctt cctagctact ttcatcaatt gtggaagatt cagcgatatt
     aagaacgttg atccgaaatc atttaaatta gtccaaaata agtatctggg agtaataatc
     cagtgtttag tgacagagac aaagacaagc gttagtaggc acatatactt ctttagcgca
     aggggtagga tcgatccact tgtatatttg gatgaatttt tgag
//