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ID   PVH51      preliminary; circular DNA; SYN; 3847 BP.
XX
AC   K03114;
XX
DT   02-MAY-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pVH51 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3847
RC   pVH51 from pVH15
RA   Chan P.T., Ohmori H., Tomizawa J.I., Lebowitz J.;
RT   "Nucleotide sequence and gene organization of ColE1 DNA";
RL   J. Biol. Chem. 260:8925-8935(1985).
XX
RN   [2]
RC   pVH51 from ColE1
RA   Lebowitz J.;
RT   ;
RL   Submitted (04-APR-1985) to GenBank on computer-readable media by:
RL   Lebowitz J., .
XX
RN   [3]
RP   1-6646
RC   from pVH51
RC   from pBR322
RA   Panayotatos N., Wells R.D.;
RT   "Cruciform structures in supercoiled DNA";
RL   Nature 289:466-470(1981).
XX
RN   [4]
RC   pML2 from pSC105 & ColE1
RC   pVH5, pVH15 from phi80 pt190 & ColE1
RA   Hershfield V., Boyer H.W., Yanofsky C., Lovett M.A., Helinski D.R.;
RT   "Plasmid ColE1 as a molecular vehicle for cloning and amplification
RT   of DNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:3455-3459(1974).
XX
RN   [5]
RC   pVH51 from pVH15
RC   pML21 from pVH51 & pSC105, ori/kan gene
RC   [pSF21 from pVH51]
RA   Hershfield V., Boyer H.W., Chow L., Helinski D.R.;
RT   "Characterization of a mini-ColE1 plasmid";
RL   J. Bacteriol. 126:447-453(1976).
XX
CC   This is the L (light) strand.
CC   NM (pVH51)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (L)(light)
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (ColE1)(phi-80)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> phi80
FT                   1. phi80
FT                   2. E. coli trp
FT                   -> phi80 trp
FT                   1. phi80
FT                   2. E. coli trp
FT                   -> phi80 pt190
FT                   1. phi80 pt190 EcoRI-EcoRI 500bp, trp
FT                   2. ColE1 EcoRI 6646bp 6645..6645
FT                   -> pVH15 7100bp
FT                   1. pVH15 remove almost all phi80 pt190, 3847bp
FT                   recombination
FT                   -> pVH51 3847bp [unique EcoRI]
FT                   1. ColE1 EcoRI-3371 3372bp 6645..6646..3371
FT                   2. phi80 trp EcoRI-EcoRI 476bp, trp
FT                   -> pVH51 3847bp [unique EcoRI]"
FT   misc_recomb     3371..3372
FT                   /note="ColE1 DNA end/phi-80 DNA start"
FT   misc_recomb     3845..3846
FT                   /note="phi-80 DNA end/ColE1 DNA start"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli kil gene"
FT   CDS             0..0
FT                   /note="GEN E. coli immunity gene"
FT   CDS             0..0
FT                   /note="GEN E. coli colicin E1 gene"
XX
SQ   Sequence 3847 BP; 983 A; 853 C; 1095 G; 916 T; 0 other;
     ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
     aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
     aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
     ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
     ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
     ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
     ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
     ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
     ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
     gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
     acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
     ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
     attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
     tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
     cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
     cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
     cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
     gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
     gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
     tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
     aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
     caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
     agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
     tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
     gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
     gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
     ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
     ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
     acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
     ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
     caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
     cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
     tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
     tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
     tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
     tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
     agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
     aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
     cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
     ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
     aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
     acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
     aagagtgggt aatggaactt ggatgaagaa agagtgaaca tgatggttcc gccatggggc
     gggcggtcat ggagttgtca ctggcagatt tacctatgac ccagcaaaac atcatcgaca
     agctggaacg gtaccggaag gaaacgggaa acgtgatagg taagggtgtg aacgggatgc
     agctgagata gtcaggaagg gtagtaaagc tgtgaagtag gcacaaaaaa cccggcgcgg
     tggccggttg ttaaagctca atatcaacta tgaaaagttt actaatagcc ccttttctaa
     ctgctgcttt ggcagttact tttaccgctg actcagtact tagacccgtt gacagaaaac
     gactaagtga aatgagatac gggttgtctg cctttgaagc agatggatcg cttatttgtg
     ctgagatgcg cttatctgaa tcatcacctt ctataatgac tttagcggtc attcggtgag
     cgtcgaa
//