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ID   PVL1393    preliminary; circular DNA; SYN; 9632 BP.
XX
AC   IG1137;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pVL1393 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc360
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pVL1393)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (British Biotechnology)(Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE ()
CC   PA (pAC360)
CC   BR (pVL1392)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAc360, ori/amp/AcMNPV polyhedrin gene
FT                   -> pVL1393 9632bp"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    868..868
FT                   /note="SIT SacII"
FT   misc_binding    1395..1395
FT                   /note="SIT ApaI"
FT   misc_binding    1901..1901
FT                   /note="SIT XhoI"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-SmaI-XbaI-EcoRI-NotI-XmaIII-PstI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 9632 BP; 2602 A; 2122 C; 2176 G; 2732 T; 0 other;
     aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
     gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
     ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
     gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
     ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
     gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
     gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
     ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
     cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
     aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
     tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
     tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
     gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
     ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
     cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
     ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
     tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccgggta
     ccttctagaa ttccggagcg gccgctgcag atctgatcct ttcctgggac ccggcaagaa
     ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt
     cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag
     cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc
     cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga
     ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga
     gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga
     gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc
     catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct
     ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
     aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt
     aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
     tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
     ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
     tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
     cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
     ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
     aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
     cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
     aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
     agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
     tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
     atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
     caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg ccgcacgccg
     ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg
     acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga
     acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc
     gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct attattgaat
     aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa tgctacgctg
     caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta taaaataatc
     aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa attgtatttg
     cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca ccagttaatg
     agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa caaccaagtg
     atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat taaaggcgag
     ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct caacgatttg
     cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata tttcgaagca
     cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc acttagcgtg
     cacgacggca cgttggagta ttttagtccg gaaaaaattc gacacacaac tatgcacgtt
     tcgtttgact ggtacgcggc gtgttaacat acaagttgct aacgtaatca tggtcatagc
     tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     agtcacgacg ttgtaaaacg acggccagtg cc
//