back Return to this vector's summary.
ID   PWB146     preliminary; circular DNA; SYN; 6082 BP.
XX
AC   M61152; M38354;
XX
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Agribacterium plasmid vector pWB146 - complete.
XX
KW   cloning vector;
KW   replication; origin; antibiotic resistance; antibiotic resistance R1.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   646-1632; 6492-7478
RC   pCaMV10 from pBR322 & cauliflower mosaic virus CM1841
RA   Gardner R.C., Howarth A.J., Hahn P., Brown-Luedi M., Shepherd R.J.,
RA   Messing J.;
RT   "The complete nucleotide sequence of an infectious clone of
RT   cauliflower mosaic virus by M13mp7 shotgun sequencing";
RL   Nucleic Acids Res. 9:2871-2888(1981).
XX
RN   [2]
RP   3346-4125
RC   pKm1 from pBR322 & Tn5, neo gene
RC   pEX205 from pBR322 & lacUV5 promoter
RC   pKm2 from pKm1 & pEX205
RC   pKm21, pKm22 from pKm2 & linker
RC   pKm24 from pKm2
RC   pKm241, pKm242, pKm243, pKm244, pKm245 from pKm24
RC   [pRZ104 from Tn5]
RA   Beck E., Ludwig G., Auerswald E.A., Reiss B., Schaller H.;
RT   "Nucleotide sequence and exact localization of the neomycin
RT   phosphotransferase gene from transposon Tn5";
RL   Gene 19:327-336(1982).
XX
RN   [3]
RP   5-505; 4855-5538
RC   from pTiT37, nopaline synthase gene
RA   Bevan M.W., Barnes W.M., Chilton M.D.;
RT   "Structure and transcription of the nopaline synthase gene region of
RT   T-DNA";
RL   Nucleic Acids Res. 11:369-385(1983).
XX
RN   [4]
RP   1743-1743
RC   lambda PI-1 from lambda & Lycopersicon proteinase inhibitor I gene
RC   lambda PI-II from lambda & Lycopersicon proteinase inhibitor I gene
RC   pTI-24 from Lycopersicon proteinase inhibitor I gene
RC   pTPI-1 from pUC9 & lambda PI-1
RA   Lee J.S., Brown W.E., Graham J.S., Pearce G., Fox E.A.,
RA   Dreher T.W., Ahern K.G., Pearson G.D.N., Ryan C.A.;
RT   "Molecular characterization and phylogenetic studies of a
RT   wound-inducible proteinase inhibitor I gene in Lycopersicon species";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:7277-7281(1986).
XX
RN   [5]
RP   1-6082
RC   pWV2 from kan gene
RC   pWB146 from pDO432 & pWV2
RC   pWB139 from pWB146 & pDO432
RC   pWB180, pWB196, pWB142 from pWB146
RC   from pRK252 & pWB146
RA   Barnes W.M.;
RT   "Variable patterns of expression of Luciferase in transgenic tobacco
RT   leaves";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:9183-9187(1990).
XX
RN   [6]
RC   pDO432 from pUC19 & pJD201 & pCaMV10 & A.tumefaciens nos gene term
RC   pDO435 from pDO432
RC   pDO445 from pDO432
RC   pDO446 from pDO432
RA   Ow D.W., Wood K.V., DeLuca M., De Wet J., Helinski D.R., Howell S.H.;
RT   "Transient and stable expression of the firefly luciferase gene in
RT   plant cells and transgenic plants";
RL   Science 234:856-859(1986).
XX
CC   NCBI gi: 209228
CC   NM (pWB146)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Agribacterium)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pRK252)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, kan gene
FT                   -> pWV2
FT                   1. firefly, luciferase gene
FT                   -> Luc23
FT                   1. firefly -XbaI, 5' luciferase gene
FT                   2. Luc23 XbaI- , 3' firefly luciferase gene
FT                   -> pJD201
FT                   1. pUC19 HindIII 2686bp 448..448, MCS
FT                   2. pCaMV10 HindIII-BamHI 1586bp 5849..7431,3' 35S prom
FT                   mutagenesis
FT                   3. pJD201 HindIII-BamHI 1886bp, luciferase gene
FT                   fill in:
FT                   BamHI linker 6bp ggatcc
FT                   4. A. tumefaciens BamHI-HindIII 1028bp, nos gene polyA
FT                   -> pDO432 7186bp
FT                   1. pDO432 HindIII-HindIII, luciferase gene
FT                   2. pWV2 HindIII-HindIII, kan gene
FT                   -> pWB146 6082bp"
FT   CDS             1660..4125
FT                   /note="luciferase gene/kanamycin resistance gene"
FT   rep_origin      0..0
FT                   /note="ORI"
XX
SQ   Sequence 6082 BP; 1626 A; 1301 C; 1321 G; 1496 T; 338 other;
     gaattcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac
     agcagcccac tcgaccttct nnccgaccca gacgagccaa gggatctttt tggaatgctg
     ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat
     gccaagcact cccgagggga accctgtggt tggcntgcac atacaaatgg acgaacggat
     aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
     caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
     ggtttctgga gtttaatgag ctaagggtac cctaagcaca tacgtcagaa accattattg
     cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
     ccactgacgt tccataaatt cccctcggta tccaattaga gtctcagatc tcctttgccc
     cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag
     aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa
     agaatgctga cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct
     acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
     aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
     gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
     ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga
     tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac
     gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact
     ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
     tatctctctc tagatctctc tcaatccaaa taatctgcaa tggcaattac cggatcttgg
     aggttactta tggatcccgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
     gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
     gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
     aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
     taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
     tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
     agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
     caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
     agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
     cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
     caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
     atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
     ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
     ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
     ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     atcctcataa aggccaagaa gggcggaaag tccaaattct cgaggggatt gcacgcaggt
     tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaagctt gctaaggaaa
     taattgagaa ggaaaatcca tccataacta atattccaat attattgagt ggttctccaa
     ttacattaga ttatctatgt gatcgagttc gtctttttga taacatcttg ggctttgttg
     tacaaatgcc tgtggtgact taattaatgg accttattat tgaagtaatt aagcagccac
     aatatgtcaa aaaataataa ttagggttca tgtatgttca ttataatgca tctcttcatg
     tactcttact atatataatg gaaaaaataa gtgtggctta attacatgta ctcctctcct
     taattaattc ctttttacat gtggtactac gttattttga ttattttaat ttatttgtct
     gattttgact ataacatctt tggacatata tttaattaat gtgcgagagc taagatttga
     attgtaattg acaaattagt cccccaaccc cactacaccc caaaaataaa aataaaacgt
     gacaatgtgt tataattttt tggaataaat taagaaggtt aagggatcaa gtaaacaaaa
     aatatgtgtg gaaagataaa tatgtattta tctttaatta gaagttttga atttgaacat
     aaatatatat aaaaaaaggt tatctattaa ttgatctact ctaaagtgga tattgaatat
     caaataaaaa actaaaaaaa agtaaagcat aacaagatct ctagaagctt ccgtcgtcga
     agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     atctatgtta ctagatcgat caaacttcgg tactgtgtaa tgacgatgag caatcgagag
     gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga attggaagtt
     tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact aattcgtcct
     gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt cttgtgaaat
     gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc ggctgataca
     ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg cctacgtcca
     agagcaagtc ctttctgtgc tcgtcggaaa ttcctctcct gtcagacggt cgtgcgcatg
     tcttgcgttg atgaagctag cttgctggcg taatagcgaa gaggcccgca ccggaattcn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaan nnnnnnnnnt gatcannnnn
     nnnnntgatc annnnnnnnn ncgatcgnnn nnnnnnncag ctgnnnnnnn nnncctnnnn
     naggnnnnnn nnnngtcgac nnnnnnnnnn cccgggnnnn nnncccgggn nnnnnnnnna
     ggcctnnnnn nnnnngaann nnttcnnnnn nnnnngcggc cgcnnnnnnn nnngcggccg
     cnnnnnnnnn ngcggccgcn nnnnnnnnng ccnnnnnggc nnnnnnnnnn gccnnnnngg
     cnnnnnnnnn ngccnnnnng gcnnnnnnnn nngccnnnnn ggcnnnnnnn nnngccnnnn
     nggcnnnnnn nnnnccgcgg nnnnnnnnnn ccgcggnnnn nnnnnnccgc ggnnnnnnnn
     nnaatattnn nnnnnnnnaa tattnnnnnn nnnnaatatt nnnnnnnnnn aatattnnnn
     nnnnnnaata ttnnnnnnnn nn
//