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ID PWE15 preliminary; circular DNA; SYN; 8136 BP.
XX
AC X65279; M99569; IG1140; ATCC37503;
XX
DT 02-JUL-1986 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Vertebrate/E.coli cosmid vector pWE15 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-8136
RC from pWE2
RC from pWE15
RC from pWE16
RA Wahl G.M., Lewis K., Ruiz J., Rothenberg B.E., Zhao J., Evans G.A.;
RT "Cosmid vectors for rapid genomic walking, restriction mapping and
RT gene transfer";
RL Proc. Natl. Acad. Sci. U.S.A. 84:2160-2164(1987).
XX
RN [2]
RC pCV103 from pJB8 & pSV2-gpt
RC pCV107 from pJB8 & pSV2-dhfr
RC pCV108 from pJB8 & pSV2-neo
RC pCL9 from pCV108 & alpha-globin gene
RC pCL1 from pCV103 & alpha-globin gene
RA Lau Y.F., Kan Y.W.;
RT "Versatile cosmid vectors for the isolation, expression, and
RT rescue of gene sequences: studies with the human alpha-globin
RT gene cluster";
RL Proc. Natl. Acad. Sci. U.S.A. 80:5225-5229(1983).
XX
RN [3]
RP 1-8164
RC pWE15 from lambda & pBR322
RA Evans G.A.;
RT ;
RL Unpublished (1989).
XX
RN [4]
RP 1-8136
RC pWE15 from lambda & pBR322
RA Voss H.;
RT ;
RL Unpublished (1992).
XX
RN [5]
RC pWE15 from lambda & pBR322
RA Wahl G.;
RT ;
RL Strategies 2:17-17(1989).
XX
RN [6]
RC pDVcos134 from lambda, cos
RA Reese J.;
RT ;
RL Unpublished (1989).
XX
CC Cosmid vector for rapid genomic walking, restriction mapping and gene
CC transfer, with selectable markers in either Escherichia coli or
CC mammalian cell lines. Contains promoters T3 and T7 flanking the BamHI
CC site. [1]
CC pWE15 (ATCC 37503) and pWE16 (ATCC 37524) differ only in the markers
CC selectable in vertebrate cells. [1]
CC Medium is 1227 LB plus ampicillin.
CC NM (pWE15)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (CLONTECH)(Stratagene)(ATCC)
CC HO (E.coli DH5)(E.coli LE392)(E.coli)(mammal)(vertebrate cells)
CC CP ()
CC FN (cloning cDNA)(mapping)
CC SE ()
CC PA (lambda)(pBR322)
CC BR (pWE16)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda, cos
FT 2. pBR322 4361bp, ori
FT 3. SV40, early region
FT 4. T7, promoter
FT 5. T3, promoter
FT 6. Tn5, neo gene
FT -> pWE15 8136bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-NotI-BamHI-NotI-EcoRI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT NotI"
FT misc_binding 0..0
FT /note="SIT unique BamHI"
FT promoter 2..19
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="SIT NotI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT ClaI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
FT misc_binding 0..0
FT /note="SIT SalI"
FT rep_origin 0..0
FT /note="ORI SV40"
FT promoter complement(0..0)
FT /note="PRO SV40 early genes"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase II gene
FT (NPTII), neomycin resistance gene (neo)
FT kanamycin resistance gene (kan)"
FT misc_binding 0..0
FT /note="SIT SfiI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BssHII"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1626..2486)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT promoter 8114..8130
FT /note="PRO bacteriophage T3"
XX
SQ Sequence 8136 BP; 2036 A; 2057 C; 2117 G; 1926 T; 0 other;
ctatagtgag tcgtattatg cggccgcgaa ttcttgaaga cgaaagggcc tcgtgatacg
cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gcttcctgtt
tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg gtttcgccac
ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
cacgatcatg cgcacccgtc agatccagac atgataagat acattgatga gtttggacaa
accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa
cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag
acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc
tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt
cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
cggatttgca ctgccggtag aactcgcgag gtcgtccagc ctcaggcagc agctgaacca
actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat ccccgcgctg
gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
tcgccatggg tcacgacgag atcctcgccg tcgggatgcg cgccttgagc ctggcgaaca
gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
agtgcttgcg gcagcgtgaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
actacttctg gaatagctca gaggccgagg cggcctaaat aaaaaaaatt agtcagccat
ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agccggatct
gcaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cgcataaatc gccgtgacga
tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcggca tcccgatgcc
gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
caagacgtag cccagcgcgt cgggccgcca tgccggcgat aatggcctgc ttctcgccga
aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
acagtccccc ggccacgggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct tggcagtcac
agcatgcgca tatccatgct tcgaccatgc gctcacaaag taggtgaatg cgcaatgtag
tacccacatc gtcatcgctt tccactgctc tcgcgaataa agatggaaaa tcaatctcat
ggtaatagtc catgaaaatc cttgtattca taaatcctcc aggtagctat atgcaaattg
aaacaaaaga gatggtgatc tttctaagag atgatggaat ctcccttcag tatcccgatg
gtcaatgcgc tggatatggg atagatggga atatgctgat ttttatggga cagagttgcg
aactgttccc aactaaaatc attttgcacg atcagcgcac tacgaacttt acccacaaat
agtcaggtaa tgaatcctga tataaagaca ggttgataaa tcagtcttct acgcgcatcg
cacgcgcaca ccgtagaaag tctttcagtt gtgagcctgg gcaaaccgtt aactttcggc
ggctttgctg tgcgacaggc tcacgtctaa aaggaaataa atcatgggtc ataaaattat
cacgttgtcc ggcgcggcga cggatgttct gtatgcgctg tttttccgtg gcgcgttgct
gtctggtgat ctgccttcta aatctggcac agccgaattg cgcgagcttg gttttgctga
aaccagacac acagcaactg aataccagaa agaaaatcac tttacctttc tgacatcaga
agggcagaaa tttgccgttg aacacctggt caatacgcgt tttggtgagc agcaatattg
cgcttcgatg acgcttggcg ttgagattga tacctctgct gcacaaaagg caatcgacga
gctggaccag cgcattcgtg acaccgtctc cttcgaactt attcgcaatg gagtgtcatt
catcaaggac gccgctatcg caaatggtgc tatccacgca gcggcaatcg aaacacctca
gccggtgacc aatatctaca acatcagcct tggtatccag cgtgatgagc cagcgcagaa
caaggtaacc gtcagtgccg ataagttcaa agttaaacct ggtgttgata ccaacattga
aacgttgatc gaaaacgcgc tgaaaaacgc tgctgaatgt gcggcgctgg atgtcacaaa
gcaaatggca gcagacaaga aagcgatgga tgaactggct tcctatgtcc gcacggccat
catgatggaa tgtttccccg gtggtgttat ctggcagcag tgccgtcgat agtatgcaat
tgataattat tatcatttgc gggtcctttc cggcgatccg ccttgttacg gggcggcgac
ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg
tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg
aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa
tggaagtcaa caaaaagcag ctggctgaca ttttcggtgc gagtatccgt accattcaga
actggcagga acagggaatg cccgttctgc gaggcggtgg caagggtaat gaggtgcttt
atgactctgc cgccgtcata aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa
agctgcgccg ggaggttgaa gaactgcggc aggccagcga ggcagatcca caggacgggt
gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
cggcggcaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagcggt caaacatgag
aattcgcggc cgcaattaac cctcactaaa ggatcc
//