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ID   PWE15      preliminary; circular DNA; SYN; 8136 BP.
XX
AC   X65279; M99569; IG1140; ATCC37503;
XX
DT   02-JUL-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli cosmid vector pWE15 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8136
RC   from pWE2
RC   from pWE15
RC   from pWE16
RA   Wahl G.M., Lewis K., Ruiz J., Rothenberg B.E., Zhao J., Evans G.A.;
RT   "Cosmid vectors for rapid genomic walking, restriction mapping and
RT   gene transfer";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:2160-2164(1987).
XX
RN   [2]
RC   pCV103 from pJB8 & pSV2-gpt
RC   pCV107 from pJB8 & pSV2-dhfr
RC   pCV108 from pJB8 & pSV2-neo
RC   pCL9 from pCV108 & alpha-globin gene
RC   pCL1 from pCV103 & alpha-globin gene
RA   Lau Y.F., Kan Y.W.;
RT   "Versatile cosmid vectors for the isolation, expression, and
RT   rescue of gene sequences: studies with the human alpha-globin
RT   gene cluster";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:5225-5229(1983).
XX
RN   [3]
RP   1-8164
RC   pWE15 from lambda & pBR322
RA   Evans G.A.;
RT   ;
RL   Unpublished (1989).
XX
RN   [4]
RP   1-8136
RC   pWE15 from lambda & pBR322
RA   Voss H.;
RT   ;
RL   Unpublished (1992).
XX
RN   [5]
RC   pWE15 from lambda & pBR322
RA   Wahl G.;
RT   ;
RL   Strategies 2:17-17(1989).
XX
RN   [6]
RC   pDVcos134 from lambda, cos
RA   Reese J.;
RT   ;
RL   Unpublished (1989).
XX
CC   Cosmid vector for rapid genomic walking, restriction mapping and gene
CC   transfer, with selectable markers in either Escherichia coli or
CC   mammalian cell lines. Contains promoters T3 and T7 flanking the BamHI
CC   site. [1]
CC   pWE15 (ATCC 37503) and pWE16 (ATCC 37524) differ only in the markers
CC   selectable in vertebrate cells. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pWE15)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (CLONTECH)(Stratagene)(ATCC)
CC   HO (E.coli DH5)(E.coli LE392)(E.coli)(mammal)(vertebrate cells)
CC   CP ()
CC   FN (cloning cDNA)(mapping)
CC   SE ()
CC   PA (lambda)(pBR322)
CC   BR (pWE16)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. pBR322 4361bp, ori
FT                   3. SV40, early region
FT                   4. T7, promoter
FT                   5. T3, promoter
FT                   6. Tn5, neo gene
FT                   -> pWE15 8136bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-NotI-BamHI-NotI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   promoter        2..19
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT SfiI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT BssHII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1626..2486)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        8114..8130
FT                   /note="PRO bacteriophage T3"
XX
SQ   Sequence 8136 BP; 2036 A; 2057 C; 2117 G; 1926 T; 0 other;
     ctatagtgag tcgtattatg cggccgcgaa ttcttgaaga cgaaagggcc tcgtgatacg
     cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gcttcctgtt
     tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg gtttcgccac
     ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     cacgatcatg cgcacccgtc agatccagac atgataagat acattgatga gtttggacaa
     accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa
     cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag
     acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc
     tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt
     cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
     aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
     gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
     agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
     aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
     aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
     caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
     actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
     cggatttgca ctgccggtag aactcgcgag gtcgtccagc ctcaggcagc agctgaacca
     actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat ccccgcgctg
     gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     tcgccatggg tcacgacgag atcctcgccg tcgggatgcg cgccttgagc ctggcgaaca
     gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
     atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     agtgcttgcg gcagcgtgaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     actacttctg gaatagctca gaggccgagg cggcctaaat aaaaaaaatt agtcagccat
     ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agccggatct
     gcaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cgcataaatc gccgtgacga
     tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
     cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcggca tcccgatgcc
     gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     caagacgtag cccagcgcgt cgggccgcca tgccggcgat aatggcctgc ttctcgccga
     aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
     cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     acagtccccc ggccacgggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct tggcagtcac
     agcatgcgca tatccatgct tcgaccatgc gctcacaaag taggtgaatg cgcaatgtag
     tacccacatc gtcatcgctt tccactgctc tcgcgaataa agatggaaaa tcaatctcat
     ggtaatagtc catgaaaatc cttgtattca taaatcctcc aggtagctat atgcaaattg
     aaacaaaaga gatggtgatc tttctaagag atgatggaat ctcccttcag tatcccgatg
     gtcaatgcgc tggatatggg atagatggga atatgctgat ttttatggga cagagttgcg
     aactgttccc aactaaaatc attttgcacg atcagcgcac tacgaacttt acccacaaat
     agtcaggtaa tgaatcctga tataaagaca ggttgataaa tcagtcttct acgcgcatcg
     cacgcgcaca ccgtagaaag tctttcagtt gtgagcctgg gcaaaccgtt aactttcggc
     ggctttgctg tgcgacaggc tcacgtctaa aaggaaataa atcatgggtc ataaaattat
     cacgttgtcc ggcgcggcga cggatgttct gtatgcgctg tttttccgtg gcgcgttgct
     gtctggtgat ctgccttcta aatctggcac agccgaattg cgcgagcttg gttttgctga
     aaccagacac acagcaactg aataccagaa agaaaatcac tttacctttc tgacatcaga
     agggcagaaa tttgccgttg aacacctggt caatacgcgt tttggtgagc agcaatattg
     cgcttcgatg acgcttggcg ttgagattga tacctctgct gcacaaaagg caatcgacga
     gctggaccag cgcattcgtg acaccgtctc cttcgaactt attcgcaatg gagtgtcatt
     catcaaggac gccgctatcg caaatggtgc tatccacgca gcggcaatcg aaacacctca
     gccggtgacc aatatctaca acatcagcct tggtatccag cgtgatgagc cagcgcagaa
     caaggtaacc gtcagtgccg ataagttcaa agttaaacct ggtgttgata ccaacattga
     aacgttgatc gaaaacgcgc tgaaaaacgc tgctgaatgt gcggcgctgg atgtcacaaa
     gcaaatggca gcagacaaga aagcgatgga tgaactggct tcctatgtcc gcacggccat
     catgatggaa tgtttccccg gtggtgttat ctggcagcag tgccgtcgat agtatgcaat
     tgataattat tatcatttgc gggtcctttc cggcgatccg ccttgttacg gggcggcgac
     ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg
     tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg
     aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa
     tggaagtcaa caaaaagcag ctggctgaca ttttcggtgc gagtatccgt accattcaga
     actggcagga acagggaatg cccgttctgc gaggcggtgg caagggtaat gaggtgcttt
     atgactctgc cgccgtcata aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa
     agctgcgccg ggaggttgaa gaactgcggc aggccagcga ggcagatcca caggacgggt
     gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     cggcggcaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagcggt caaacatgag
     aattcgcggc cgcaattaac cctcactaaa ggatcc
//