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ID   PWE15A     preliminary; circular DNA; SYN; 8213 BP.
XX
AC   Z12112;
XX
DT   18-MAY-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pWE15A - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8213
RC   pWE15A from pWE15 & oligo, Sp6 promoter & T7 promoter
RA   Seto D., Koop B.F., Seto J., Hood L.E.;
RT   "Complete nucleotide sequence of the cosmid vector pWE15A";
RL   Nucleic Acids Res. 20:3786-3786(1992).
XX
RN   [2]
RC   pWE15A from pWE15 & oligo, Sp6 promoter & T7 promoter
RA   Lai E., Wang K., Avdalovic N., Hood L.E.;
RT   "Rapid restriction map constructions using a modified pWE15 cosmid
RT   vector and a robotic workstation";
RL   Biotechniques 11:212-217(1991).
XX
CC   NM (pWE15A)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning up to 40000 bp)
CC   SE ()
CC   PA (pWE15)
CC   BR ()
CC   OF ()
CC   OR ()
XX
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pWE15 EcoRI 8136bp 29..29
FT                   2. oligo EcoRI-Sp6 prom-BamHI-T7 prom-EcoRI 70bp
FT                   -> pWE15A 8213bp"
FT   promoter        complement(1..16)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    19..67
FT                   /note="MCS SmaI-ApaI-NseI-NarI-MluI-SalI-SfiI-EaqI-
FT                   NotI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
FT   misc_feature    0..0
FT                   /note="from SV2neo"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    8146..8190
FT                   /note="MCS NotI-SfiI-XhoI-PvuI-NruI-SacI-ScaI-SacII"
FT   promoter        8193..8205
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    8208..8213
FT                   /note="SIT BamHI"
XX
SQ   Sequence 8213 BP; 2041 A; 2088 C; 2151 G; 1933 T; 0 other;
     ctatagtgag tcgtattacc cgggcccgcc ggcgccacgc gtcgacggcc ggccgggccg
     cggccgcgaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     tcgcccttat tccctttttt gcggcatttt gcttcctgtt tttgctcacc cagaaacgct
     ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     gagataccta cagcgtgagc tatgagaaag cgccacgctt ccgaagggag aaaggcggac
     aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     aacgcctggt atctttatag tcctgtcggg gtttcgccac ctctgacttg agcgtcgatt
     tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgctgacttc cgcgtttcca
     gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
     tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtc
     agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     tctctagtca aggcactata catcaaatat tccttattaa cccctttaca aattaaaaag
     ctaaaggtac acaatttttg agcatagtta ttaatagcag acactctatg cctgtgtgga
     gtaagaaaaa acagtatgtt atgattataa ctgttatgcc tacttataaa ggttacagaa
     tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt gtaaatagca
     aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag caattctgaa
     ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga atgttgagag
     tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag gttttcctca
     ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat ctaaaataca
     caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat atttacctta
     gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa gtaaggttcc
     ttcacaaaga tccggaccaa agcggccatc gtgcctcccc actcctgcag ttcgggggca
     tggatgcgcg gatagccgct gctggtttcc tggatgccga cggatttgca ctgccggtag
     aactcgcgag gtcgtccagc ctcaggcagc agctgaacca actcgcgagg ggatcgagcc
     cggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
     gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     atcctcgccg tcgggatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
     cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
     agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     gaggccgagg cggcctaaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     tttccacacc ctaactgaca cacattccac agccggatct gcaggaccca acgctgcccg
     agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
     gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
     gttccatgtg ctcgccgagg cgcataaatc gccgtgacga tcagcggtcc aatgatcgaa
     gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     tgcctggaca gcatggcctg caacgcggca tcccgatgcc gccggaagcg agaagaatca
     taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     cgggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct
     cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggc
     ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     cggccacgat gcgtccggcg tagaggatct tggcagtcac agcatgcgca tatccatgct
     tcgaccatgc gctcacaaag taggtgaatg cgcaatgtag tacccacatc gtcatcgctt
     tccactgctc tcgcgaataa agatggaaaa tcaatctcat ggtaatagtc catgaaaatc
     cttgtattca taaatcctcc aggtagctat atgcaaattg aaacaaaaga gatggtgatc
     tttctaagag atgatggaat ctcccttcag tatcccgatg gtcaatgcgc tggatatggg
     atagatggga atatgctgat ttttatggga cagagttgcg aactgttccc aactaaaatc
     attttgcacg atcagcgcac tacgaacttt acccacaaat agtcaggtaa tgaatcctga
     tataaagaca ggttgataaa tcagtcttct acgcgcatcg cacgcgcaca ccgtagaaag
     tctttcagtt gtgagcctgg gcaaaccgtt aactttcggc ggctttgctg tgcgacaggc
     tcacgtctaa aaggaaataa atcatgggtc ataaaattat cacgttgtcc ggcgcggcga
     cggatgttct gtatgcgctg tttttccgtg gcgcgttgct gtctggtgat ctgccttcta
     aatctggcac agccgaattg cgcgagcttg gttttgctga aaccagacac acagcaactg
     aataccagaa agaaaatcac tttacctttc tgacatcaga agggcagaaa tttgccgttg
     aacacctggt caatacgcgt tttggtgagc agcaatattg cgcttcgatg acgcttggcg
     ttgagattga tacctctgct gcacaaaagg caatcgacga gctggaccag cgcattcgtg
     acaccgtctc cttcgaactt attcgcaatg gagtgtcatt catcaaggac gccgctatcg
     caaatggtgc tatccacgca gcggcaatcg aaacacctca gccggtgacc aatatctaca
     acatcagcct tggtatccag cgtgatgagc cagcgcagaa caaggtaacc gtcagtgccg
     ataagttcaa agttaaacct ggtgttgata ccaacattga aacgttgatc gaaaacgcgc
     tgaaaaacgc tgctgaatgt gcggcgctgg atgtcacaaa gcaaatggca gcagacaaga
     aagcgatgga tgaactggct tcctatgtcc gcacggccat catgatggaa tgtttccccg
     gtggtgttat ctggcagcag tgccgtcgat agtatgcaat tgataattat tatcatttgc
     gggtcctttc cggcgatccg ccttgttacg gggcggcgac ctcgcgggtt ttcgctattt
     atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg tcataactta atgtttttat
     ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg aaagcgagct ttttggcctc
     tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag
     ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga acagggaatg
     cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc cgccgtcata
     aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
     gaactgcggc aggccagcga ggcagatcca caggacgggt gtggtcgcca tgatcgcgta
     gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggcaaa gcggtcggac
     agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
     ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
     ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
     ccgcattaaa gcttatcgat gataagcggt caaacatgag aattcgcggc cgcggcctcg
     agggccgatc gcgagctcag tactccgcgg atttaggtga cactatagga tcc
//