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ID PWE15A preliminary; circular DNA; SYN; 8213 BP.
XX
AC Z12112;
XX
DT 18-MAY-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector pWE15A - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-8213
RC pWE15A from pWE15 & oligo, Sp6 promoter & T7 promoter
RA Seto D., Koop B.F., Seto J., Hood L.E.;
RT "Complete nucleotide sequence of the cosmid vector pWE15A";
RL Nucleic Acids Res. 20:3786-3786(1992).
XX
RN [2]
RC pWE15A from pWE15 & oligo, Sp6 promoter & T7 promoter
RA Lai E., Wang K., Avdalovic N., Hood L.E.;
RT "Rapid restriction map constructions using a modified pWE15 cosmid
RT vector and a robotic workstation";
RL Biotechniques 11:212-217(1991).
XX
CC NM (pWE15A)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning up to 40000 bp)
CC SE ()
CC PA (pWE15)
CC BR ()
CC OF ()
CC OR ()
XX
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pWE15 EcoRI 8136bp 29..29
FT 2. oligo EcoRI-Sp6 prom-BamHI-T7 prom-EcoRI 70bp
FT -> pWE15A 8213bp"
FT promoter complement(1..16)
FT /note="PRO bacteriophage T7"
FT misc_binding 19..67
FT /note="MCS SmaI-ApaI-NseI-NarI-MluI-SalI-SfiI-EaqI-
FT NotI"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
FT misc_feature 0..0
FT /note="from SV2neo"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 8146..8190
FT /note="MCS NotI-SfiI-XhoI-PvuI-NruI-SacI-ScaI-SacII"
FT promoter 8193..8205
FT /note="PRO bacteriophage Sp6"
FT misc_binding 8208..8213
FT /note="SIT BamHI"
XX
SQ Sequence 8213 BP; 2041 A; 2088 C; 2151 G; 1933 T; 0 other;
ctatagtgag tcgtattacc cgggcccgcc ggcgccacgc gtcgacggcc ggccgggccg
cggccgcgaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
tcgcccttat tccctttttt gcggcatttt gcttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
gagataccta cagcgtgagc tatgagaaag cgccacgctt ccgaagggag aaaggcggac
aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
aacgcctggt atctttatag tcctgtcggg gtttcgccac ctctgacttg agcgtcgatt
tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgctgacttc cgcgtttcca
gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtc
agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
tctctagtca aggcactata catcaaatat tccttattaa cccctttaca aattaaaaag
ctaaaggtac acaatttttg agcatagtta ttaatagcag acactctatg cctgtgtgga
gtaagaaaaa acagtatgtt atgattataa ctgttatgcc tacttataaa ggttacagaa
tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt gtaaatagca
aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag caattctgaa
ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga atgttgagag
tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag gttttcctca
ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat ctaaaataca
caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat atttacctta
gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa gtaaggttcc
ttcacaaaga tccggaccaa agcggccatc gtgcctcccc actcctgcag ttcgggggca
tggatgcgcg gatagccgct gctggtttcc tggatgccga cggatttgca ctgccggtag
aactcgcgag gtcgtccagc ctcaggcagc agctgaacca actcgcgagg ggatcgagcc
cggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
atcctcgccg tcgggatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
gaggccgagg cggcctaaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
tttccacacc ctaactgaca cacattccac agccggatct gcaggaccca acgctgcccg
agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
gttccatgtg ctcgccgagg cgcataaatc gccgtgacga tcagcggtcc aatgatcgaa
gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
tgcctggaca gcatggcctg caacgcggca tcccgatgcc gccggaagcg agaagaatca
taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
cgggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct
cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggc
ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
cggccacgat gcgtccggcg tagaggatct tggcagtcac agcatgcgca tatccatgct
tcgaccatgc gctcacaaag taggtgaatg cgcaatgtag tacccacatc gtcatcgctt
tccactgctc tcgcgaataa agatggaaaa tcaatctcat ggtaatagtc catgaaaatc
cttgtattca taaatcctcc aggtagctat atgcaaattg aaacaaaaga gatggtgatc
tttctaagag atgatggaat ctcccttcag tatcccgatg gtcaatgcgc tggatatggg
atagatggga atatgctgat ttttatggga cagagttgcg aactgttccc aactaaaatc
attttgcacg atcagcgcac tacgaacttt acccacaaat agtcaggtaa tgaatcctga
tataaagaca ggttgataaa tcagtcttct acgcgcatcg cacgcgcaca ccgtagaaag
tctttcagtt gtgagcctgg gcaaaccgtt aactttcggc ggctttgctg tgcgacaggc
tcacgtctaa aaggaaataa atcatgggtc ataaaattat cacgttgtcc ggcgcggcga
cggatgttct gtatgcgctg tttttccgtg gcgcgttgct gtctggtgat ctgccttcta
aatctggcac agccgaattg cgcgagcttg gttttgctga aaccagacac acagcaactg
aataccagaa agaaaatcac tttacctttc tgacatcaga agggcagaaa tttgccgttg
aacacctggt caatacgcgt tttggtgagc agcaatattg cgcttcgatg acgcttggcg
ttgagattga tacctctgct gcacaaaagg caatcgacga gctggaccag cgcattcgtg
acaccgtctc cttcgaactt attcgcaatg gagtgtcatt catcaaggac gccgctatcg
caaatggtgc tatccacgca gcggcaatcg aaacacctca gccggtgacc aatatctaca
acatcagcct tggtatccag cgtgatgagc cagcgcagaa caaggtaacc gtcagtgccg
ataagttcaa agttaaacct ggtgttgata ccaacattga aacgttgatc gaaaacgcgc
tgaaaaacgc tgctgaatgt gcggcgctgg atgtcacaaa gcaaatggca gcagacaaga
aagcgatgga tgaactggct tcctatgtcc gcacggccat catgatggaa tgtttccccg
gtggtgttat ctggcagcag tgccgtcgat agtatgcaat tgataattat tatcatttgc
gggtcctttc cggcgatccg ccttgttacg gggcggcgac ctcgcgggtt ttcgctattt
atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg tcataactta atgtttttat
ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg aaagcgagct ttttggcctc
tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag
ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga acagggaatg
cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc cgccgtcata
aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
gaactgcggc aggccagcga ggcagatcca caggacgggt gtggtcgcca tgatcgcgta
gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggcaaa gcggtcggac
agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
ccgcattaaa gcttatcgat gataagcggt caaacatgag aattcgcggc cgcggcctcg
agggccgatc gcgagctcag tactccgcgg atttaggtga cactatagga tcc
//