back Return to this vector's summary.
ID   PWL812     preliminary; circular DNA; SYN; 5214 BP.
XX
AC   IG9835;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pWL812 - complete, replication origin at R1.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pWL7 from ColE1 & kan gene
RC   pRK101, pRK102, pRK103, pRK107 from pWL812 & pBR322
RC   [pRK103b from pRK103]
RC   pRK104 from pWL812 & pWL7
RA   Kollek R., Oertel W., Goebel W.;
RT   "Isolation and characterization of the minimal fragment required
RT   for autonomous replication (basic replicon) of a copy mutant
RT   (pKN102) of the antibiotic resistance factor R1";
RL   Mol. Gen. Genet. 162:51-57(1978).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pWL812)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Rsc13)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Rsc13 PstI-PstI 5214bp 2784..7894..104,
FT                   \ ori/amp gene/PstI E/F frags
FT                   \ PstI = 104 646 1189 2784 3404 6253 6911
FT                   -> pWL812 5214bp"
FT   -               1..5214
FT                   /note="Rsc13 2784..7894..103 5214bp
FT                   PstI = CTGCA^G"
FT   rep_origin      0..0
FT                   /note="ORI"
XX
SQ   Sequence 5214 BP; 1290 A; 1284 C; 1311 G; 1329 T; 0 other;
     gtttatgctg gttatgctgg ctgcatgggg cattagttcg ggagccagat ttgtcatgga
     gcaggccgtt ctgctttatg gatcaggaaa aaactatttg ttcttcagtc atggtactgt
     tctgatgtac ctgctgtgtg ttttcctggt atacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttttataaac
     cgtggagcgg gcaatactga gctgatgagc aatttccgtt gcaccagtgc ccttctgatg
     aagcgtcagc acgacgttcc tgtccacggt acgcctgcgg ccaaatttga ttcctttcag
     ctttgcttcc tgtcggccct cattcgtgcg ctctaggatc ctccggcgtt cagcctgtgc
     cacagccgac aggatggtga ccaccatttg ccccatatca ccgtcggtac tgatcccgtc
     gtcaataaac cgaaccgcta caccctgagc atcaaactct tttatcagtt ggatcatgtc
     ggcggtgtcg cggccaagac ggtcgagctt cttcaccaga atgacatcac cttcctccac
     cttcatcctc agcaaatcca gcccttcccg atctgttgaa ctgccggatg ccttgtcggt
     aaagatgcgg ttagctttta cccctgcatc tttgagcgct ctgatctgaa tatcgaggga
     ctgctggctg gttgagaccc gcgcataacc aaaaattcgc ataaaaatgt accttaaatc
     gaatatcaga cacgatgtgt ctattatgcc aaaatgacga tttaatggac actcaaacga
     agccgtttta ctatgtctga taatttataa tatttcgaac ggttgcagtt gtgttaaaaa
     agccgtcagg cagggaggcc gatatgcccg ttgatttttt gaccactgag caggttgaga
     gttatggcag gttcactggc gaacccgatg aacttcagct ggcgcgttat tttcatcttg
     atgaagcgga taaagaattt atcgggaaaa gccggggtga tcacaatcga cttggtattg
     ccctgcaaat cgggtgtgtg cgttttctgg gcacttttct tactgacatg aatcatattc
     cttccggcgt ccggcatttt accgccagac agctcgggat tcgtgatatc accgttcttg
     cagaatacgg tcagagggaa aatacccgcc gtgagcatgc agcgctgata cgtcagcact
     atcagtatcg tgaatttgcc tggccctgga catttcgcct tacccgtctt ttatataccc
     ggagctggat aagcaacgaa cgtcctggcc tgcttttcga cctggcgaca gggtggctta
     tgcaacatcg tattattctc cccggagcca ccacgctgac ccggttgatt tcagaggtaa
     gggaaaaggc gacgttgcgc ctgtggaaca aactggcact gataccgtca gccgaacagc
     gttcacagct ggagatgctg ctggggccaa ctgattgcag ccgcctgtct ttactggaat
     cactgaaaaa aggccctgtg accatcagtg gtccggcgtt taatgaagca attgaacgct
     ggaaaactct gaacgatttt ggcctgcatg ctgaaaacct gagtacactc ccggctgtgc
     gcctgaaaaa tctcgcacgt tatgctggta tgacttcggt gttcaatatt gccaggatgt
     caccgcagaa aaggatggcg gttctggttg cctttgtcct tgcatgggaa acgctggcgc
     tggatgatgc actggacgtt ctggacgcca tgctggccgt tatcatccgt gacgccagaa
     agattgggca gaaaaaacgg ctccgctcgc tgaaggatct ggataaatct gcattggcgc
     tcgccagcgc atgttcgtac ttgctgaaag aagaaacacc ggacgaatcg attcgtgctg
     aggtgttcag ctacatccct aggcaaaagc tggctgaaat catcacgctt gtccgtgaaa
     ttgcccggcc ctcagacgat aattttcatg acgaaatggt ggagcagtac gggcgcgttc
     gtcgtttcct gccccatctg ctgaataccg ttaaattttc atccgcacct gccggggtta
     ccactctgaa tgcctgtgac tacctcagcc gggagttcag ctcacggcgg cagttttttg
     acgacgcacc aacggaaatc atcagtcagt catggaaacg gctggtgatt aacaaggaaa
     aacatatcac ccgaagggga tacacgctct gctttctcag taaactgcag gatagtctga
     gacggaggga tgtctacgtt accggcagta accggtgggg agatcctcgt gcaagattac
     tacagggtgc tgactggcag gcaaatcgga ttaaggttta tcgttctttg gggcacccga
     cagacccgca ggaagcaata aaatctctgg gccatcagct tgatagtcgt tacagacagg
     ttgctgcacg tcttggcgaa aatgaggctg tcgaactcga tgtttctggc ccgaagcccc
     ggttgacaat ttctcccctc gccagtcttg atgagccgga cagtctgaaa cgactgagca
     aaatgatcag tgatctgctc cctccggtgg atttaacgga gttgctgctc gaaattaacg
     cccataccgg atttgctgat gagtttttcc atgccagtga agccagtgcc agagttgatg
     atctgcccgt cagcatcagc gccgtgctga tggctgaagc ctgcaatatc ggtctggaac
     cactgatcag atcaaatgtt cctgcactga cccgacaccg gctgaactgg acaaaagcga
     actatctgcg ggctgaaact atcaccagcg ctaatgccag actggttgat tttcaggcaa
     cgctgccact ggcacagata tggggtggag gagaagtggc atctgcagat ggaatgcgct
     ttgttacgcc agtcagaaca atcaatgccg gaccgaaccg caaatacttt ggtaataaca
     gagggatcac ctggtacaac tttgtgtccg atcagtattc cggctttcat ggctttcatg
     gcatcgttat accggggacg ctgagggact ctatctttgt gctggaaggc cttctggaac
     aggagaccgg gctgaatcca accgaaatta tgaccgatac ggcaggtgcc agcgatcttg
     tctttggcct tttctggctg ctgggatacc agttttctcc acgcctggct gatgccggtg
     cttcggtttt ctggcgaatg gaccatgatg ccgactatgg cgtgctgaat gatattgcca
     gagggcaatc agatccccga aaaatagtcc ttcagtggga cgaaatgatc cggaccgcag
     gctccctgaa gctgggcaaa gtacaggcct cagtgctggt ccgttcattg ctgaaaagtg
     aacgtccctc cggactgact caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt
     atctgcttaa ttatattgat gatgaagatt accgccggcg cattctgacc cagcttaatc
     ggggagaaag ccgtcatgca gttgccagag ccatctgtca cggtcaaaaa ggtgagataa
     gaaaacgata taccgacggt caggaagatc agttgggagc tctggggctg gtcactaacg
     ccgtcgtgtt atggaacact atttatatgc aggcagctct ggatcatctc cgggcgcagg
     gtgaaacact gaatgatgaa gatatcgcac ggctctcccc gctttgccac ggacatatca
     atatgctcgg ccattattcc ttcacgctgg cagaactggt gaccaaaggt catctgagac
     cattaaaaga ggcgtcagag gcagaaaacg ttgcttaacg tgagttttcg ttccactgag
     cgtcagaccc catttacgac ctgaacgcag acgccgggat attcttctgg aagaggttct
     gaggcaggct ggtattccgt tgctcagaag tcacgatgcc agaaaactgc tgca
//