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ID   PWP14      preliminary; circular DNA; SYN; 3068 BP.
XX
AC   IG9859;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pWP14 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pWP14 from pUC9 & lambda, cos
RC   pWP32 from pUC9 & lambda, cos 154
RA   Shinder G., Gold M.;
RT   "The Nul subunit of bacteriophage lambda terminase binds to
RT   specific sites in cos DNA";
RL   J. Virol. 62:387-392(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pWP14)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC9)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC9 HincII 2665bp 250..250
FT                   2. lambda EcoRI-EcoRI 393bp, cos
FT                   \ 21227 26105 31748 39169 44973
FT                   HincII-HincII 403bp 48299..48502..200, lambda cos
FT                   -> pWP14 3068bp"
FT   -               1..249
FT                   /note="pUC9 1..249 249bp
FT                   HindII = HincII = GTY^RAC"
FT   -               250..652
FT                   /note="lambda 48299..48502..199 403bp
FT                   HindII = HincII = GTY^RAC"
FT   -               653..3068
FT                   /note="pUC9 250..2665 2416bp"
FT   misc_binding    0..0
FT                   /note="MCS PstI-SalI-BamHI-SmaI-EcoRI; EcoRI-BamHI-
FT                   SalI"
FT   misc_binding    0..0
FT                   /note="SIT double BamHI-EcoRI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3068 BP; 744 A; 755 C; 765 G; 804 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acatgaggtt gccccgtatt cagtgtcgct gatttgtatt gtctgaagtt
     gtttttacgt taagttgatg cagatcaatt aatacgatac ctgcgtcata attgattatt
     tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta gatgataatc attatcactt
     tacgggtcct ttccggtgat ccgacaggtt acggggcggc gacctcgcgg gttttcgcta
     tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac ttaatgtttt
     tatttaaaat accctctgaa aagaaaggaa acgacaggtg ctgaaagcga ggctttttgg
     cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tcgacggatc
     cccgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     gcggaaga
//