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ID   PWT571     preliminary; circular DNA; SYN; 3779 BP.
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AC   L09155; VB0089;
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DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pWT571 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3779
RC   pWT571
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pWT221 from pAT153 & pWT121
RC   pWT501 from pAT153 & pWT221
RC   pWT511 from pWT501
RC   pWT550 from pAT153 & pWT501
RC   pWT551 from pWT550
RC   pWT551-2P2, pWT551-3P3 from pWT551
RC   pWT570 from pAT153 & pWT501
RC   pWT571 from pWT570
RC   pMN39-1 from pWT501 & human beta-interferon
RA   Tacon W., Bonass W.A., Jenkins B., Emtage J.S.;
RT   "Expression plasmid vectors containing Escherichia coli
RT   tryptophan promoter transcriptional units lacking the attenuator";
RL   Gene 23:255-265(1983).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pAT153 and GenBank:EcoTrpPO according to the
CC   publication cited by F. Pfeiffer.
CC   There is confliciting information on the structure of pAT153.
CC   some 200 bases may be erraneuosly missing from this sequence.
CC
CC   The construction of three different types of trp promoter
CC   expression plasmids (pWT511, pWT551 and pWT571) is described.
CC   The Tc-resistance gene is under the control of the Trp-operator.
CC   pWT571 contains the cloning site directly after the ATG start
CC   codon.
CC   NM (pUR278)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAT153)(EcoTrpPO)(EcoTrp)
CC   BR (pWT511)(pWT551)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. ptrp ED5-1, E. coli trp operon
FT                   -> pWT101
FT                   1. pWT101
FT                   -> pWT111
FT                   1. pWT111
FT                   -> pWT121
FT                   1. pAT153 3658bp
FT                   2. pWT121, E. coli trp operon
FT                   -> pWT221
FT                   1. pAT153 HindIII 3658bp 30..30
FT                   2. pWT221 HhaI-HhaI 139bp, E. coli trp operon
FT                   DNA polymerase I:DNA polymerase I
FT                   HindIII linker 10bp ccaagcttgg:HindIII linker 10bp
FT                   \ ccaagcttgg
FT                   -> pWT501
FT                   1. pAT153 EcoRI 3658bp 3657..3657
FT                   2. pWT501 HaeIII-HaeIII 137bp, trp promoter
FT                   HaeIII-TaqI 99bp, trp promoter
FT                   :DNA polymerase I
FT                   :EcoRI linker 6bp gaattc
FT                   -> pWT570 [unique HindIII]"
FT                   1. pWT570 EcoRI, 5' to trp promoter
FT                   DNA polymerase I
FT                   -> pWT571 3779bp [unique EcoRI]"
FT   misc_feature    1..10
FT                   /note="destroyed EcoRI linker"
FT   misc_feature    16..124
FT                   /note="from E. coli trp operon"
FT   misc_feature    125..3779
FT                   /note="from pAT153"
FT   CDS             210..1400
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(2717..3505)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3779 BP; 855 A; 1033 C; 974 G; 917 T; 0 other;
     gaattaattc attgtccgac atcacaacgg ttctggcaaa tattctgaaa tgagctgttg
     acaattaatc atcgaactag ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa
     tgaattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt
     taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc
     tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
     cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
     gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
     tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     ttcgtaaagt ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
//