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ID   PXA1       preliminary; circular DNA; SYN; 4972 BP.
XX
AC   IG2021;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pXa1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   ptac or ptac12deltaEco from ptac12
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pXa1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Boehringer)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-3X)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-3X 4952bp, factor Xa protease site
FT                   -> pXa1 4972bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum glutathione
FT                   S-transferase gene (GST)"
FT   misc_binding    0..0
FT                   /note="SIT factor Xa"
XX
SQ   Sequence 4972 BP; 1228 A; 1201 C; 1293 G; 1250 T; 0 other;
     agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgaattcccg ggtcgactcg
     agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac
     gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt
     ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt
     acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
     aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
     gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc
     gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
     aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt
//