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ID   PXA2       preliminary; circular DNA; SYN; 4973 BP.
XX
AC   IG2022;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pXa2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   ptac or ptac12deltaEco from ptac12
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pXa2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-3X)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-3X 4952bp, factor Xa protease site
FT                   -> pXa2 4973bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum glutathione
FT                   S-transferase gene (GST)"
FT   misc_binding    0..0
FT                   /note="SIT factor Xa"
XX
SQ   Sequence 4973 BP; 1228 A; 1201 C; 1294 G; 1250 T; 0 other;
     agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg atcgaaggtc gtgggatccc cggaattccc gggtcgactc
     gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa
     aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata
     cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat
     tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag
     agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
     ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
     acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
     caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
     cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
     gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
     cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
     attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
     acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
     ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
     gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
     gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
     aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
     atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
     gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt
     tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc
     cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga att
//