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ID   PXA3       preliminary; circular DNA; SYN; 4974 BP.
XX
AC   IG2023;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pXa3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   ptac or ptac12deltaEco from ptac12
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pXa3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-3X)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-3X 4952bp, factor Xa protease site
FT                   -> pXa3 4974bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum glutathione
FT                   S-transferase gene (GST)"
FT   misc_binding    0..0
FT                   /note="SIT factor Xa"
XX
SQ   Sequence 4974 BP; 1229 A; 1201 C; 1294 G; 1250 T; 0 other;
     agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg atcgaaggtc gtgggatccc caggaattcc cgggtcgact
     cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
     aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
     acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
     ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
     ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
     ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt
//