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ID   PXT5       preliminary; circular DNA; SYN; 5121 BP.
XX
AC   IG9846;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pXT5 - complete.
XX
KW   cloning vector; insertion sequence.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pXT5 from ColE1 & pBR322
RC   pSZ7 from pXT5
RA   Zhiang S., Yan L., Zubay G.;
RT   "Regulation of gene expression in plasmid ColE1: delayed expression
RT   of the kil gene";
RL   J. Bacteriol. 170:5460-5467(1988).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pXT5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (ColE1)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 remove XmnI-XmnI 2948bp 1684..4632,
FT                   \ 3698bp
FT                   2. pBR322 EcoRI-AvaI 1427bp 4360..4361..1426, tet gene
FT                   blunt end:blunt end
FT                   -> pXT5 5125bp"
FT   -               1..1683
FT                   /note="ColE1 1..1683 1683bp
FT                   XmnI =   GAANN^NNTTC
FT                   EcoRI = G^AATT C"
FT   -               1684..3106
FT                   /note="pBR322 3..1425 1423bp
FT                   AvaI =     C^YCGRG
FT                   XmnI = GAANN^NNTTC"
FT   -               3107..5121
FT                   /note="ColE1 4632..6646 2015bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5121 BP; 1288 A; 1207 C; 1316 G; 1310 T; 0 other;
     ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
     aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
     aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
     ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
     ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
     ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
     ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
     ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
     ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
     gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
     acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
     ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
     attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
     tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
     cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
     cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     aagctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa
     attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg
     gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
     tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat
     atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc
     gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga
     ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga
     tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatcgcgt tctgtctgct
     gatcatgtgg attttctgca tgtccctcac tgtgcctttc tggtgtctca ttctgcttta
     attcgccgta tgcccggatc agttcactgg tttcaaactc ccgtcgtccg tccctgccgg
     tacggtaact gacaaggcct ccgcgcatat cacggtaaaa ctgtgaacgc gatctgcctg
     tcatttttag tgcgtcccgg agtgtgtgcc atgccataaa gtgacagtgt cccatagatg
     tctcatctca tagtttcagt aaaacataat gaggtctgag aacggtaatg tttgtgctgg
     tttttgtggc atcgggcgag aatagcgcgt ggtgtgaaag actgtttttt tgatcgtttt
     cacaaaaatg gaagtccaca gtcttgacag ggaaaatgca gcggcgtagc ttttatgctg
     tatataaaac cagtggttat atgtacagta tttattttta acttattgtt ttaaaagtca
     aagaggattt tataatggaa accgcggtag cgtactataa agatggtgtt ccttatgatg
     ataagggaca ggtaattatt actcttttga atggtactcc tgacgggagt ggctctggcg
     gcggaggtgg aaaaggaggc agtaaaagtg aaagttctgc agctattcat gcaactgcta
     aatggtctac tgctcaatta aagaaaacac aggcagagca ggctgcccgg gcaaaagctg
     cagcggaagc acaggcgaaa gcaaaggcaa acagggatgc gctgactcag cgcctgaagg
     atatcgtgaa tgaggctctt cgtcacaatg cctcacgtac gccttcagca acagagcttg
     ctcatgctaa taatgcagct atgcaggcgg aagcagagcg tttgcgcctt gcgaaagcag
     aagaaaaagc ccgtaaagaa gcggaagcag cagaaaaggc ttttcaggaa gcagaacaac
     gacgtaaaga gattgaacgg gagaaggctg aaacagaacg ccagttgaaa ctggctgaag
     ctgaagagaa acgactggct gcattgagtg aagaagctaa agctgttgag atcgcccaaa
     aaaaactttc tgctgcacaa tctgaagtgg tgaaaatgga tggagagatt aagactctca
     attctcgttt aagctccagt atccatgccc gtgatgcaga aatgaaaacg ctcgctggaa
     aacgaaatga actggctcag gcatccgcta aatataaaga actggatgag ctggtcaaaa
     aactatcacc aagagccaat gatccgcttc agaaccgtcc tttttttgaa gcaaccagac
     gacgggttgg ggccggtaag attagagaag aaaaacaaaa acaggtaaca gcatcagaaa
     cacgtattaa ccggataaat gctgatataa ctcagatcca gaaggctatt tctcaggtca
     gtaataatcg taatgccggt atcgctcgtg ttcatgaagc tgaagaaaat ttgaaaaaag
     cacagaataa tctccttaat tcacagatta aggatgctgt tgatgcaaca gttagctttt
     atcaaacgct gactgaaaaa tatggtgaaa aatattcgaa aatggcacag gaacttgctg
     ataagtctaa aggtaagaaa atcggcaatg tgaatgaagc tctcgctgct tttgaaaaat
     acaaggatgt tttaaataag aaattcagca aagccgatcg tgatgctatt tttaatgcgt
     tggcatcggt gaagtatgat gactgggcta aacatttaga tcagtttgcc aagtacttga
     agattacggg gcatgtttct tttggatatg atgtggtatc tgatatccta aaaattaagg
     atacaggtga ctggaagcca ctatttctta cattagagaa gaaagctgca gatgcagggg
     tgagttatgt tgttgcttta ctttttagct tgcttgctgg aactacatta ggtatttggg
     gtattgctat tgttacagga a
//