Return to this vector's summary.
ID PYAC3 preliminary; circular DNA; SYN; 11446 BP.
XX
AC U01086; ATCC37520;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli YAC vector pYAC3 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from YIp5
RC pPM662 from plasmid & YCp19
RC pPM664 from pPM662 & linker & pSU4-A, sup4-o gene
RC pPM668 from pPM664
RC pYAC2 from pPM668 & linker & A240p1
RC pYAC3 from pYAC2
RC pYAC4 from pYAC3 & linker
RC pYAC5, pYAC55 from pYAC3 & linker
RA Burke D.T., Carle G.F., Olson M.V.;
RT "Cloning of large segments of exogenous DNA into yeast by means of
RT artificial chromosome vectors";
RL Science 236:806-812(1987).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC pYAC3 was constructed from pYAC2 by replacing the SmaI site in the
CC SUP4 intron with the SnaBI site, and eliminating the SfiI and NotI
CC sites flanking the SUP4 gene.
CC The HIS3 sequence is removed when producing the telomers by BamHI
CC digestion.
CC A yeast artificial chromosome vector.
CC The SnaBI site, in the intron of SUP4ochre, inactivates SUP4ochre.
CC Medium is 1227 LB plus ampicillin.
CC NM (pYAC3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (yeast artificial chromosome)
CC SP (ATCC)
CC HO (E.coli)(Saccharomyces cerevisiae)
CC HO (E.coli RR1)(Saccharomyces cerevisiae AB1380)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (YIp5)(YCp19)(yeast sup4-o gene)(lambda)(yeast telomere)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pYAC2 11420bp, SUP4-o gene intron [11100bp]
FT \ pYAC4 503..597 SUP4-o
FT mutagenesis, to make SnaBI at 554..554
FT SfiI 11420bp <503, 5' to SUP4-o
FT fill in
FT NotI 11426bp <503, 5' to SUP4-o
FT fill in
FT SfiI 11434bp >597, 3' to SUP4-o
FT fill in
FT NotI 11440bp >597, 3' to SUP4-o
FT fill in
FT -> pYAC3 11448bp [11700bp; SnaBI at 554]"
FT - 1..552
FT /note="pYAC4 1..552 552bp
FT SnaBI = TAC^GTA
FT \ ggaattcc"
FT - 553..11446
FT /note="pYAC4 561..11454 10894bp"
FT misc_binding 0..0
FT /note="SIT unique SalI-ClaI-SmaI-SnaBI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI yeast ARS1"
FT misc_feature 0..0
FT /note="yeast centromere CEN4"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
FT CDS 0..0
FT /note="ANT yeast SUP4 gene (ochre suppressor)"
FT CDS 0..0
FT /note="ANT yeast TRP1 gene"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
XX
SQ Sequence 11446 BP; 3148 A; 2548 C; 2564 G; 3186 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
atttatcact acgtaatctt gagatcgggc gttcgatcgc cccgggagat ttttttgttt
tttatgtctt ccattcactt cccagacttg caagttgaaa tatttctttc aagggaattg
atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc
aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg
atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
ccatctccag cagccgcacg cggcgcatcc ccccccccct ttcaattcaa ttcatcattt
tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc ggtaatctcc
gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt
agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc
tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat
cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt
gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc
aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca
gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa
tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca
gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg
aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg
tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag
agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa
ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg agacgcattg
ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt
ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa
gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa
gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta
ctcgggcgta atgattttta taatgacgaa aaaaaaaaaa ttggaaagaa aagggggggg
gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
catcctctct cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg
aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gccctcgagg gataagcttc
atttttagat aaaatttatt aatcatcatt aatttcttga aaaacatttt atttattgat
cttttataac aaaaaaccct tctaaaagtt tatttttgaa tgaaaaactt ataaaaattt
atgaaaacta caaaaaataa aatttttaat taaaataatt ttgataagaa cttcaatctt
tgactagcta gcttagtcat ttttgagatt taattaatat tttatgttta ttcatatata
aactattcaa aatattatag aatttaaaca ttttaacatc ttaatcattc ataaataact
aaaaatcaaa gtattacatc aataaataac ttttactcaa tgtcaaagaa ttattggggt
tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
tggggttggg gttggggttg gggtgggaaa acagcattca ggtattagaa gaatatcctg
attcaggtga aaatattgtt gatgcgcggg atcctcgggg acaccaaata tggcgatctc
ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac caccagaacg
gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc
acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc cgccgttgtt
gttattgtag ttgctcatgt tatttctggc acttcttggt tttcctctta agtgaggagg
aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc
agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc cacctatcac
cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg atcgagttca
agagaaaaaa aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca
cgtatagaat gatgcattac cttgtcatct tcagtatcat actgttcgta tacatactta
ctgacattca taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa
taatcggtgt cactacataa gaacaccttt ggtggaggga acatcgttgg taccattggg
cgaggtggct tctcttatgg caaccgcaag agccttgaac gcactctcac tacggtgatg
atcattcttg cctcgcagac aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct
ttcaagaaaa tgcgggatca tctcgcaaga gagatctcct actttctccc tttgcaaacc
aagttcgaca actgcgtacg gcctgttcga aagatctacc accgctctgg aaagtgcctc
atccaaaggc gcaaatcctg atccaaacct ttttactcca cgcgccagta gggcctcttt
aaaagcttga ccgagagcaa tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa
gtcaccaatg cactcaacga ttagcgacca gccggaatgc ttggccagag catgtatcat
atggtccaga aaccctatac ctgtgtggac gttaatcact tgcgattgtg tggcctgttc
tgctactgct tctgcctctt tttctgggaa gatcgagtgc tctatcgcta ggggaccacc
ctttaaagag atcgcaatct gaatcttggt ttcatttgta atacgcttta ctagggcttt
ctgctctgtc atctttgcct tcgtttatct tgcctgctca ttttttagta tattcttcga
agaaatcaca ttactttata taatgtataa ttcattatgt gataatgcca atcgctaaga
aaaaaaaaga gtcatccgct aggtggaaaa aaaaaaatga aaatcattac cgaggcataa
aaaaatatag agtgtactag aggaggccaa gagtaataga aaaagaaaat tgcgggaaag
gactgtgtta tgacttccct gactaatgcc gtgttcaaac gatacctggc agtgactcct
agcgctcacc aagctcttaa aacgagaatt aagaaaaagt cgtcatcttt cgataagttt
ttcccacagc aaagcaatag tagaaaaaaa caatgggaaa cgttgaatga agacaaagcg
tcgtggttta aaaggaaata cgctcacgta catgctaggg aacaggaccg tgcagcggat
cccgcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tgaatgctgt
tttcccaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
accccaaccc caaccccaac cccaacccca accccaataa ttctttgaca ttgagtaaaa
gttatttatt gatgtaatac tttgattttt agttatttat gaatgattaa gatgttaaaa
tgtttaaatt ctataatatt ttgaatagtt tatatatgaa taaacataaa atattaatta
aatctcaaaa atgactaagc tagctagtca aagattgaag ttcttatcaa aattatttta
attaaaaatt ttattttttg tagttttcat aaatttttat aagtttttca ttcaaaaata
aacttttaga agggtttttt gttataaaag atcaataaat aaaatgtttt tcaagaaatt
aatgatgatt aataaatttt atctaaaaat gaagcttatc cctcgagggc tgcctcgcgc
gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta
ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt
aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc
gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga
aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa
aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat
gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg
tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa
aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgcct gcaggccttt
tgaaaagcaa gcataaaaga tctaaacata aaatctgtaa aataacaaga tgtaaagata
atgctaaatc atttggcttt ttgattgatt gtacaggaaa atatacatcg cagggggttg
acttttacca tttcaccgca atggaatcaa acttgttgaa gagaatgttc acaggcgcat
acgctacaat gacccgattc ttgctagcct tttctcggtc ttgcaaacaa ccgccggcag
cttagtatat aaatacacat gtacatacct ctctccgtat cctcgtaatc attttcttgt
atttatcgtc ttttcgctgt aaaaacttta tcacacttat ctcaaataca cttattaacc
gcttttacta ttatcttcta cgctgacagt aatatcaaac agtgacacat attaaacaca
gtggtttctt tgcataaaca ccatcagcct caagtcgtca agtaaagatt tcgtgttcat
gcagatagat aacaatctat atgttgataa ttagcgttgc ctcatcaatg cgagatccgt
ttaaccggac cctagtgcac ttaccccacg ttcggtccac tgtgtgccga acatgctcct
tcactatttt aacatgtgga attaattcta aatcctcttt atatgatctg ccgatagata
gttctaagtc attgaggttc atcaacaatt ggattttctg tttactcgac ttcaggtaaa
tgaaatgaga tgatacttgc ttatctcata gttaactcta agaggtgata cttatttact
gtaaaactgt gacgataaaa ccggaaggaa gaataagaaa actcgaactg atctataatg
cctattttct gtaaagagtt taagctatga aagcctcggc attttggccg ctcctaggta
gtgctttttt tccaaggaca aaacagtttc tttttcttga gcaggtttta tgtttcggta
atcataaaca ataaataaat tatttcattt atgtttaaaa ataaaaaata aaaaagtatt
ttaaattttt aaaaaagttg attataagca tgtgaccttt tgcaagcaat taaattttgc
aatttgtgat tttaggcaaa agttacaatt tctggctcgt gtaatatatg tatgctaaag
tgaactttta caaagtcgat atggacttag tcaaaagaaa ttttcttaaa aatatatagc
actagccaat ttagcacttc tttatgagat atattataga ctttattaag ccagatttgt
gtattatatg tatttacccg gcgaatcatg gacatacatt ctgaaatagg taatattctc
tatggtgaga cagcatagat aacctaggat acaagttaaa agctagtact gttttgcagt
aatttttttc ttttttataa gaatgttacc acctaaataa gttataaagt caatagttaa
gtttgatatt tgattgtaaa ataccgtaat atatttgcat gatcaaaagg ctcaatgttg
actagccagc atgtcaacca ctatattgat caccgatata tggacttcca caccaactag
taatatgaca ataaattcaa gatattcttc atgagaatgg cccaga
//