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ID   PYAC3      preliminary; circular DNA; SYN; 11446 BP.
XX
AC   U01086; ATCC37520;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli YAC vector pYAC3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from YIp5
RC   pPM662 from plasmid & YCp19
RC   pPM664 from pPM662 & linker & pSU4-A, sup4-o gene
RC   pPM668 from pPM664
RC   pYAC2 from pPM668 & linker & A240p1
RC   pYAC3 from pYAC2
RC   pYAC4 from pYAC3 & linker
RC   pYAC5, pYAC55 from pYAC3 & linker
RA   Burke D.T., Carle G.F., Olson M.V.;
RT   "Cloning of large segments of exogenous DNA into yeast by means of
RT   artificial chromosome vectors";
RL   Science 236:806-812(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   pYAC3 was constructed from pYAC2 by replacing the SmaI site in the
CC   SUP4 intron with the SnaBI site, and eliminating the SfiI and NotI
CC   sites flanking the SUP4 gene.
CC   The HIS3 sequence is removed when producing the telomers by BamHI
CC   digestion.
CC   A yeast artificial chromosome vector.
CC   The SnaBI site, in the intron of SUP4ochre, inactivates SUP4ochre.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pYAC3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (yeast artificial chromosome)
CC   SP (ATCC)
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae AB1380)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YIp5)(YCp19)(yeast sup4-o gene)(lambda)(yeast telomere)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pYAC2 11420bp, SUP4-o gene intron [11100bp]
FT                   \ pYAC4 503..597 SUP4-o
FT                   mutagenesis, to make SnaBI at 554..554
FT                   SfiI 11420bp <503, 5' to SUP4-o
FT                   fill in
FT                   NotI 11426bp <503, 5' to SUP4-o
FT                   fill in
FT                   SfiI 11434bp >597, 3' to SUP4-o
FT                   fill in
FT                   NotI 11440bp >597, 3' to SUP4-o
FT                   fill in
FT                   -> pYAC3 11448bp [11700bp; SnaBI at 554]"
FT   -               1..552
FT                   /note="pYAC4 1..552 552bp
FT                   SnaBI = TAC^GTA
FT                   \           ggaattcc"
FT   -               553..11446
FT                   /note="pYAC4 561..11454 10894bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-ClaI-SmaI-SnaBI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast SUP4 gene (ochre suppressor)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 11446 BP; 3148 A; 2548 C; 2564 G; 3186 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
     ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
     ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
     atttatcact acgtaatctt gagatcgggc gttcgatcgc cccgggagat ttttttgttt
     tttatgtctt ccattcactt cccagacttg caagttgaaa tatttctttc aagggaattg
     atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc
     aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg
     atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
     taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
     atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
     tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
     ccatctccag cagccgcacg cggcgcatcc ccccccccct ttcaattcaa ttcatcattt
     tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc ggtaatctcc
     gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt
     agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc
     tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat
     cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt
     gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc
     aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca
     gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa
     tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca
     gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg
     aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg
     tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag
     agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa
     ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg agacgcattg
     ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt
     ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa
     gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa
     gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta
     ctcgggcgta atgattttta taatgacgaa aaaaaaaaaa ttggaaagaa aagggggggg
     gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     catcctctct cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg
     aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gccctcgagg gataagcttc
     atttttagat aaaatttatt aatcatcatt aatttcttga aaaacatttt atttattgat
     cttttataac aaaaaaccct tctaaaagtt tatttttgaa tgaaaaactt ataaaaattt
     atgaaaacta caaaaaataa aatttttaat taaaataatt ttgataagaa cttcaatctt
     tgactagcta gcttagtcat ttttgagatt taattaatat tttatgttta ttcatatata
     aactattcaa aatattatag aatttaaaca ttttaacatc ttaatcattc ataaataact
     aaaaatcaaa gtattacatc aataaataac ttttactcaa tgtcaaagaa ttattggggt
     tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     tggggttggg gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt
     tggggttggg gttggggttg gggtgggaaa acagcattca ggtattagaa gaatatcctg
     attcaggtga aaatattgtt gatgcgcggg atcctcgggg acaccaaata tggcgatctc
     ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac caccagaacg
     gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc
     acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc cgccgttgtt
     gttattgtag ttgctcatgt tatttctggc acttcttggt tttcctctta agtgaggagg
     aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc
     agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc cacctatcac
     cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg atcgagttca
     agagaaaaaa aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca
     cgtatagaat gatgcattac cttgtcatct tcagtatcat actgttcgta tacatactta
     ctgacattca taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa
     taatcggtgt cactacataa gaacaccttt ggtggaggga acatcgttgg taccattggg
     cgaggtggct tctcttatgg caaccgcaag agccttgaac gcactctcac tacggtgatg
     atcattcttg cctcgcagac aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct
     ttcaagaaaa tgcgggatca tctcgcaaga gagatctcct actttctccc tttgcaaacc
     aagttcgaca actgcgtacg gcctgttcga aagatctacc accgctctgg aaagtgcctc
     atccaaaggc gcaaatcctg atccaaacct ttttactcca cgcgccagta gggcctcttt
     aaaagcttga ccgagagcaa tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa
     gtcaccaatg cactcaacga ttagcgacca gccggaatgc ttggccagag catgtatcat
     atggtccaga aaccctatac ctgtgtggac gttaatcact tgcgattgtg tggcctgttc
     tgctactgct tctgcctctt tttctgggaa gatcgagtgc tctatcgcta ggggaccacc
     ctttaaagag atcgcaatct gaatcttggt ttcatttgta atacgcttta ctagggcttt
     ctgctctgtc atctttgcct tcgtttatct tgcctgctca ttttttagta tattcttcga
     agaaatcaca ttactttata taatgtataa ttcattatgt gataatgcca atcgctaaga
     aaaaaaaaga gtcatccgct aggtggaaaa aaaaaaatga aaatcattac cgaggcataa
     aaaaatatag agtgtactag aggaggccaa gagtaataga aaaagaaaat tgcgggaaag
     gactgtgtta tgacttccct gactaatgcc gtgttcaaac gatacctggc agtgactcct
     agcgctcacc aagctcttaa aacgagaatt aagaaaaagt cgtcatcttt cgataagttt
     ttcccacagc aaagcaatag tagaaaaaaa caatgggaaa cgttgaatga agacaaagcg
     tcgtggttta aaaggaaata cgctcacgta catgctaggg aacaggaccg tgcagcggat
     cccgcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tgaatgctgt
     tttcccaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
     accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
     accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
     accccaaccc caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca
     accccaaccc caaccccaac cccaacccca accccaataa ttctttgaca ttgagtaaaa
     gttatttatt gatgtaatac tttgattttt agttatttat gaatgattaa gatgttaaaa
     tgtttaaatt ctataatatt ttgaatagtt tatatatgaa taaacataaa atattaatta
     aatctcaaaa atgactaagc tagctagtca aagattgaag ttcttatcaa aattatttta
     attaaaaatt ttattttttg tagttttcat aaatttttat aagtttttca ttcaaaaata
     aacttttaga agggtttttt gttataaaag atcaataaat aaaatgtttt tcaagaaatt
     aatgatgatt aataaatttt atctaaaaat gaagcttatc cctcgagggc tgcctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta
     ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt
     aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc
     gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga
     aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa
     aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat
     gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg
     tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
     gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
     gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
     ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa
     aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
     gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
     taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgcct gcaggccttt
     tgaaaagcaa gcataaaaga tctaaacata aaatctgtaa aataacaaga tgtaaagata
     atgctaaatc atttggcttt ttgattgatt gtacaggaaa atatacatcg cagggggttg
     acttttacca tttcaccgca atggaatcaa acttgttgaa gagaatgttc acaggcgcat
     acgctacaat gacccgattc ttgctagcct tttctcggtc ttgcaaacaa ccgccggcag
     cttagtatat aaatacacat gtacatacct ctctccgtat cctcgtaatc attttcttgt
     atttatcgtc ttttcgctgt aaaaacttta tcacacttat ctcaaataca cttattaacc
     gcttttacta ttatcttcta cgctgacagt aatatcaaac agtgacacat attaaacaca
     gtggtttctt tgcataaaca ccatcagcct caagtcgtca agtaaagatt tcgtgttcat
     gcagatagat aacaatctat atgttgataa ttagcgttgc ctcatcaatg cgagatccgt
     ttaaccggac cctagtgcac ttaccccacg ttcggtccac tgtgtgccga acatgctcct
     tcactatttt aacatgtgga attaattcta aatcctcttt atatgatctg ccgatagata
     gttctaagtc attgaggttc atcaacaatt ggattttctg tttactcgac ttcaggtaaa
     tgaaatgaga tgatacttgc ttatctcata gttaactcta agaggtgata cttatttact
     gtaaaactgt gacgataaaa ccggaaggaa gaataagaaa actcgaactg atctataatg
     cctattttct gtaaagagtt taagctatga aagcctcggc attttggccg ctcctaggta
     gtgctttttt tccaaggaca aaacagtttc tttttcttga gcaggtttta tgtttcggta
     atcataaaca ataaataaat tatttcattt atgtttaaaa ataaaaaata aaaaagtatt
     ttaaattttt aaaaaagttg attataagca tgtgaccttt tgcaagcaat taaattttgc
     aatttgtgat tttaggcaaa agttacaatt tctggctcgt gtaatatatg tatgctaaag
     tgaactttta caaagtcgat atggacttag tcaaaagaaa ttttcttaaa aatatatagc
     actagccaat ttagcacttc tttatgagat atattataga ctttattaag ccagatttgt
     gtattatatg tatttacccg gcgaatcatg gacatacatt ctgaaatagg taatattctc
     tatggtgaga cagcatagat aacctaggat acaagttaaa agctagtact gttttgcagt
     aatttttttc ttttttataa gaatgttacc acctaaataa gttataaagt caatagttaa
     gtttgatatt tgattgtaaa ataccgtaat atatttgcat gatcaaaagg ctcaatgttg
     actagccagc atgtcaacca ctatattgat caccgatata tggacttcca caccaactag
     taatatgaca ataaattcaa gatattcttc atgagaatgg cccaga
//